Abstract
Viral neutralization tests (VNTs) have long been considered old-fashioned tricks in the armamentarium of fundamental virology, with laboratory implementation for a limited array of viruses only. Nevertheless, they represent the most reliable surrogate of potency for passive immunotherapies, such as monoclonal or polyclonal antibody therapy. The recent interest around therapy with convalescent plasma or monoclonal antibodies for the Covid-19 pandemic has paralleled the revival of VNTs. We review here the available methods by dissecting variations for each fundamental component of the VNT (i.e., virus type and dose, replication-competent cell line, serum, and detection system).
Keywords: convalescent plasma; high through put neutralizing antibodies; turnaround time; viral neutralization.
【저자키워드】 convalescent plasma, viral neutralization, high through put neutralizing antibodies, turnaround time, 【초록키워드】 viruses, convalescent plasma, pandemic, therapy, Neutralizing antibodies, antibody, COVID-19 pandemic, monoclonal antibody, Variation, monoclonal antibodies, virus, Laboratory, Replication, serum, Viral, neutralization test, implementation, Neutralizing, viral neutralization, variations, convalescent, monoclonal, dose, neutralization tests, polyclonal antibody, polyclonal antibody therapy, immunotherapies, potency, cell line, VNT, polyclonal, detection system, Viral neutralization tests, 【제목키워드】 neutralization, Infection, Viral, Convalescent plasma therapy, implication,