The hypothesis that hepatitis B infection is etiologically related to hepatoma has been investigated by studying the interrelationships between hepatitis B surface antigen (HBsAg, Australia antigen) and the fast-moving 5′-nucleotide phosphodiesterase Band V isoenzyme (5′-NPDase-V). Sera from 58 patients with viral hepatitis were tested for 5′-NPDase-V and HBsAg. The isoenzyme was found in 34 of 37 patients who were also positive for HBsAg but in only 4 of 21 hepatitis patients who were HBsAg negative. Five patients convalescing from hepatitis were negative for both HBsAg and the isoenzyme. Preparative gel electrophoresis showed that these 2 markers were different proteins. Of 34 hepatoma patients, 29 were positive for 5′-NPDase-V. Only 1 isoenzyme-positive patient was positive for HBsAg by counterimmunoelectrophoresis. However, of 16 isoenzyme-positive hepatoma patients available for radioimmunoassay, 8 were NBsAg positive (50%). None of 21 hepatoma samples tested for antibody to NBsAg was positive. Of 21 “normal” carriers of HBsAg and 10 carriers with Down’s syndrome, 4 persons were detected with the isoenzyme. The results suggest that HBsAg and 5′-NPDase-V in the presence of liver damage are associated and thus provide a new marker enzyme between hepatitis B infection and hepatoma.
5′-nucleotide phosphodiesterase isoenzyme in patients with hepatitis B infection
[Category] B형 간염,
[Source] pubmed
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