Abstract
The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer-spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus.
【초록키워드】 Coronavirus infection, ACE2, Diagnosis, virus, angiotensin-converting enzyme 2, Spike protein, Antigen, aptamer, Protein, DNA, outbreak, SARS-CoV-2 spike protein, limit of detection, gold, membrane, virus infection, baculovirus, Protein binding, binding, SARS-CoV-2 spike, Angiotensin-converting enzyme, angiotensin, Receptor binding, energy transfer, rhodamine, pseudotyped, recombinant antigen, demonstrated, GNS, persist, 【제목키워드】 detection, Corona, nanoparticle, surface, energy, Spectroscopy, Conjugated,