The infection of coronavirus disease (COVID-19) seriously threatens human life. It is urgent to generate effective and safe specific antibodies (Abs) against the pathogenic elements of COVID-19. Mice were immunized with SARS-CoV-2 spike protein antigens: S ectodomain-1 (CoV, in short) mixed in Alum adjuvant for 2 times and boosted with CoV weekly for 6 times. A portion of mice were treated with Maotai liquor (MTL, in short) or/and heat stress (HS) together with CoV boosting. We observed that the anti-CoV Ab was successfully induced in mice that received the CoV/Alum immunization for 2 times. However, upon boosting with CoV, the CoV Ab production diminished progressively; spleen CoV Ab-producing plasma cell counts reduced, in which substantial CoV-specific Ab-producing plasma cells (sPC) were apoptotic. Apparent oxidative stress signs were observed in sPCs; the results were reproduced by exposing sPCs to CoV in the culture. The presence of MTL or/and HS prevented the CoV-induced oxidative stress in sPCs and promoted and stabilized the CoV Ab production in mice in re-exposure to CoV. In summary, CoV/Alum immunization can successfully induce CoV Ab production in mice that declines upon reexposure to CoV. Concurrent administration of MTL/HS stabilizes and promotes the CoV Ab production in mice.
【초록키워드】 COVID-19, coronavirus disease, Stress, Infection, oxidative stress, immunization, Spike protein, Culture, mice, SARS-CoV-2 spike protein, CoV, plasma, spleen, Plasma cell, specific antibodies, Plasma cells, administration, Decline, alum, Safe, heat stress, pathogenic, portion, cell count, element, alum adjuvant, apoptotic, protein antigens, concurrent, effective, immunized, specific antibody, generate, reduced, treated, promote, induce, prevented, promoted, Maotai, 【제목키워드】 Superoxide, induction, Specific,