The COVID-19 pandemic caused by SARS-CoV-2 is a continuous challenge worldwide, and there is an urgent need to map the landscape of immunogenic and immunodominant epitopes recognized by CD8 + T cells. Here, we analyze samples from 31 patients with COVID-19 for CD8 + T cell recognition of 500 peptide-HLA class I complexes, restricted by 10 common HLA alleles. We identify 18 CD8 + T cell recognized SARS-CoV-2 epitopes, including an epitope with immunodominant features derived from ORF1ab and restricted by HLA-A*01:01. In-depth characterization of SARS-CoV-2-specific CD8 + T cell responses of patients with acute critical and severe disease reveals high expression of NKG2A, lack of cytokine production and a gene expression profile inhibiting T cell re-activation and migration while sustaining survival. SARS-CoV-2-specific CD8 + T cell responses are detectable up to 5 months after recovery from critical and severe disease, and these responses convert from dysfunctional effector to functional memory CD8 + T cells during convalescence. Many viral antigens have been identified in patients with COVID-19 patients, but which of these result in meaningful immune responses is unclear. Here the authors identify a range of SARS-CoV-2 CD8 + T cell responses across patients including a response targeting an epitope of ORF1ab with immunodominant properties.
【저자키워드】 Adaptive immunity, Infectious diseases, CD8-positive T cells, 【초록키워드】 SARS-CoV-2, immune response, T cells, COVID-19 pandemic, CD8, memory, Epitopes, T cell, Migration, survival, immune responses, response, Patient, convalescence, epitope, expression, Critical, patients, T cell response, SARS-CoV-2 epitopes, cytokine production, immunodominant epitopes, COVID-19 patients, NKG2A, severe disease, ORF1ab, HLA-A, Viral antigen, viral antigens, HLA alleles, landscape, immunogenic, gene expression profile, ORF1, immunodominant, immunodominant epitope, feature, identify, lack, caused, detectable, functional, complexes, reveal, inhibiting, patients with COVID-19, 【제목키워드】 SARS-CoV-2, CD8, identification, immunodominant, feature,