Abstract
Low concentrations of type-I interferon (IFN) in blood seem to be associated with more severe forms of Coronavirus disease 2019 (COVID-19). However, following the type-I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature on a FilmArray® system, which includes PCR assays for four interferon stimulated genes. We analyzed three types of patient populations: (i) children admitted to a pediatric emergency unit for fever and suspected infection, (ii) ICU-admitted patients with severe COVID-19, and (iii) healthcare workers with mild COVID-19. The results were compared to the reference tools, that is, molecular signature assessed with Nanostring® and IFN-α2 quantification by SIMOA® (Single MOlecule Array). A strong correlation was observed between the IR measured by the FilmArray®, Nanostring®, and SIMOA® platforms (r-Spearman 0.996 and 0.838, respectively). The FilmArray® panel could be used in the COVID-19 pandemic to evaluate the IR in 45-min with 2 min hand-on-time at hospitalization and to monitor the IR in future clinical trials.
Keywords: COVID-19; Clinical application; FilmArray®; In vitro diagnostic; Interferon.
【저자키워드】 COVID-19, interferon, Clinical application, FilmArray®, In vitro diagnostic, 【초록키워드】 Coronavirus disease 2019, severe COVID-19, Hospitalization, pediatric, children, COVID-19 pandemic, Infection, interferon, clinical trials, healthcare worker, clinical, Fever, Patient, Mild, IFN, molecular, correlation, quantification, disease, interferon response, early stage, platform, Blood, Concentration, IFN-α2, PCR assay, MONITOR, Genes, stimulated, analyzed, evaluate, include, evaluated, form, 【제목키워드】 SARS-CoV-2, viral infection, PCR, platform,