Abstract
Globally, there is an urgency to develop effective, low-cost therapeutic interventions for coronavirus disease 2019 (COVID-19). We previously generated the stable and ultrapotent homotrimeric Pittsburgh inhalable Nanobody 21 (PiN-21). Using Syrian hamsters that model moderate to severe COVID-19 disease, we demonstrate the high efficacy of PiN-21 to prevent and treat SARS-CoV-2 infection. Intranasal delivery of PiN-21 at 0.6 mg/kg protects infected animals from weight loss and substantially reduces viral burdens in both lower and upper airways compared to control. Aerosol delivery of PiN-21 facilitates deposition throughout the respiratory tract and dose minimization to 0.2 mg/kg. Inhalation treatment quickly reverses animals’ weight loss after infection, decreases lung viral titers by 6 logs leading to drastically mitigated lung pathology, and prevents viral pneumonia. Combined with the marked stability and low production cost, this innovative therapy may provide a convenient and cost-effective option to mitigate the ongoing pandemic.
【초록키워드】 COVID-19, Treatment, coronavirus disease, Efficacy, pandemic, therapy, SARS-COV-2 infection, Infection, aerosol, Viral pneumonia, stability, inhalation, respiratory tract, hamster, upper airway, moderate, severe COVID-19 disease, Lung pathology, dose, weight loss, therapeutic intervention, treat, urgency, viral burden, Pittsburgh, mitigate, Prevent, effective, decrease, Combined, PROTECT, log, develop, facilitate, reduce, lung viral titer, 【제목키워드】 SARS-COV-2 infection, hamster, dose, treat, Prevent,