Understanding the mechanism of protective immunity in the nasal mucosae is central to the design of more effective vaccines that prevent nasal infection and transmission of Bordetella pertussis . We found significant infiltration of IL-17-secreting CD4 + tissue-resident memory T (T RM ) cells and Siglec-F + neutrophils into the nasal tissue during primary infection with B. pertussis . Il17A −/− mice had significantly higher bacterial load in the nasal mucosae, associated with significantly reduced infiltration of Siglec-F + neutrophils. Re-infected convalescent mice rapidly cleared B. pertussis from the nasal cavity and this was associated with local expansion of IL-17-producing CD4 + T RM cells. Depletion of CD4 T cells from the nasal tissue during primary infection or after re-challenge of convalescent mice significantly delayed clearance of bacteria from the nasal mucosae. Protection was lost in Il17A −/− mice and this was associated with significantly less infiltration of Siglec-F + neutrophils and antimicrobial peptide (AMP) production. Finally, depletion of neutrophils reduced the clearance of B. pertussis following re-challenge of convalescent mice. Our findings demonstrate that IL-17 plays a critical role in natural and acquired immunity to B. pertussis in the nasal mucosae and this effect is mediated by mobilizing neutrophils, especially Siglec-F + neutrophils, which have high neutrophil extracellular trap (NET) activity.
IL-17 mediates protective immunity against nasal infection with Bordetella pertussis by mobilizing neutrophils, especially Siglec-F + neutrophils
IL-17는 Siglec-F + 호중구를 특히 동원하여 Bordetella pertussis에 의한 비강 감염에 대한 보호 면역을 매개합니다.
[Category] 백일해,
[Article Type] Article
[Source] PMC
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