Efficacious interventions are urgently needed for the treatment of COVID-19. Here, we report a monoclonal antibody (mAb), MW05, with SARS-CoV-2 neutralizing activity by disrupting the interaction of receptor binding domain (RBD) with angiotensin-converting enzyme 2 (ACE2) receptor. Crosslinking of Fc with FcγRIIB mediates antibody-dependent enhancement (ADE) activity by MW05. This activity is eliminated by introducing the LALA mutation to the Fc region (MW05/LALA). Potent prophylactic and therapeutic effects against SARS-CoV-2 are observed in rhesus monkeys. A single dose of MW05/LALA blocks infection of SARS-CoV-2 in prophylactic treatment and clears SARS-CoV-2 in three days in a therapeutic treatment setting. These results pave the way for the development of MW05/LALA as an antiviral strategy for COVID-19. Here the authors characterize a monoclonal antibody from a COVID-19 convalescent patient that interferes with SARS-CoV-2 spike binding to ACE2 and has prophylactic and therapeutic activity in non-human primates. Antibody-dependent enhancement of infection is prevented by mutating the Fc region of the antibody.
【저자키워드】 antibodies, SARS-CoV-2, Translational immunology, 【초록키워드】 COVID-19, Treatment, ACE2, Mutation, Antiviral, antibody, Antibody-dependent enhancement, monoclonal antibody, Infection, Intervention, Prophylactic, angiotensin-converting enzyme 2, Receptor binding domain, Neutralizing activity, RBD, non-human primates, receptor, ADE, potent, convalescent patient, single dose, binding, SARS-CoV-2 spike, mAb, Interaction, Rhesus monkeys, therapeutic effect, Fc region, therapeutic activity, prophylactic treatment, Therapeutic treatment, FcγRIIb, block, LALA, interfere, prevented, disrupting, eliminated, 【제목키워드】 neutralizing antibody, Prophylactic, characterization, therapeutic efficacy, Rhesus monkey,