This study describes a case of SARS-CoV-2 reinfection confirmed by whole-genome sequencing in a healthy physician who had been working in a COVID-19 hospital in Italy since the beginning of the pandemic. Nasopharyngeal swabs were obtained from the patient at each presentation as part of routine surveillance. Nucleic acid amplification testing was performed on the two samples to confirm SARS-CoV-2 infection, and serological tests were used to detect SARS-CoV-2 IgG antibodies. Comparative genome analysis with whole-genome sequencing was performed on nasopharyngeal swabs collected during the two episodes of COVID-19. The first COVID-19 episode was in March 2020, and the second was in January 2021. Both SARS-CoV-2 infections presented with mild symptoms, and seroconversion for SARS-CoV-2 IgG was documented. Genomic analysis showed that the viral genome from the first infection belonged to the lineage B.1.1.74, while that from the second infection to the lineage B.1.177. Epidemiological, clinical, serological, and genomic analyses confirmed that the second episode of SARS-CoV-2 infection in the healthcare worker met the qualifications for “best evidence” for reinfection. Further studies are urgently needed to assess the frequency of such a worrisome occurrence, particularly in the light of the recent diffusion of SARS-CoV-2 variants of concern.
【저자키워드】 COVID-19, whole-genome sequencing, SARS-COV-2 infection, Reinfection, clades-lineages, anti-SARS-CoV-2 IgG antibodies, 【초록키워드】 whole-genome sequencing, serological test, SARS-CoV-2, pandemic, SARS-COV-2 infection, hospital, Genome, SARS-CoV-2 variant, Infection, Italy, healthcare worker, Nasopharyngeal swab, amplification, Seroconversion, Viral, Serological tests, Surveillance, SARS-CoV-2 variants, IgG antibodies, nasopharyngeal swabs, Lineage, nucleic acid amplification testing, Genomic analysis, Swab, genomic, serological, SARS-CoV-2 infections, SARS-CoV-2 IgG, mild symptoms, Frequency, Analysis, viral genome, episode of, Comparative, SARS-CoV-2 IgG antibodies, diffusion, B.1.177, Occurrence, detect, collected, healthy, the patient, was performed, were used, 【제목키워드】 SARS-CoV-2, worker, Confirmed by,