Summary For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared with the often applied plaque-forming unit assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel. Graphical abstract Highlights • The protocol describes assessment of neutralization of SARS-CoV-2 by antibodies • Neutralization is detected by means of reduced cytopathic effect on adherent cells • Increased safety for researchers through less manipulation of infectious cultures • The described protocol allows for more efficient upscaling of sample testing For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared with the often applied plaque-forming unit assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel.
【저자키워드】 immunology, Microbiology, Cell-based Assays, Cell culture, 【초록키워드】 SARS-CoV-2, coronavirus, protocol, antibody, neutralization, virus, serum, Culture, therapeutic, respiratory, convalescent, Cytopathic effect, neutralization capacity, therapeutic antibodies, tissue culture, acute respiratory syndrome, Abstract, plaque-forming unit, acute respiratory syndrome coronavirus, tissue, cell cultures, adherent cells, researcher, cytopathic, tested, described, required, applied, reduced, less, Increased, adherent, adherent cell, 【제목키워드】 protocol, Culture, serum antibody, SARS-CoV-2 neutralization, tissue, adherent cell,