A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2’) known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.
All Keywords
【초록키워드】 COVID-19, public health, SARS-CoV-2, coronavirus, pandemic, furin, Antibody Response, peptide, Spike protein, Epitopes, stability, Patient, plasma, cellular entry, respiratory, epitope, Critical, S1/S2 cleavage site, acute respiratory syndrome, acute respiratory syndrome coronavirus, S1/S2, biochemical evidence, positive, immunodominant, linear epitopes, consequence, inhibit, the spike protein, the antibody response, proteolytic, 【제목키워드】 SARS-CoV-2, Epitopes, Patient, identification, immunodominant, linear epitope,
【초록키워드】 COVID-19, public health, SARS-CoV-2, coronavirus, pandemic, furin, Antibody Response, peptide, Spike protein, Epitopes, stability, Patient, plasma, cellular entry, respiratory, epitope, Critical, S1/S2 cleavage site, acute respiratory syndrome, acute respiratory syndrome coronavirus, S1/S2, biochemical evidence, positive, immunodominant, linear epitopes, consequence, inhibit, the spike protein, the antibody response, proteolytic, 【제목키워드】 SARS-CoV-2, Epitopes, Patient, identification, immunodominant, linear epitope,
신종 중증급성호흡기증후군 코로나바이러스(SARS-CoV-2)는 공중 보건과 경제적 안정에 치명적인 결과를 초래하는 현재 대유행(COVID-19)의 원인입니다. 펩타이드 어레이를 사용하여 치유 환자(12)와 건강한 환자(6)로부터 혈장의 항체 반응을 매핑하여 3개의 면역우세 선형 에피토프를 확인했으며, 그 중 2개는 스파이크 단백질(S1/S2 및 S2')의 주요 단백질 분해 부위에 해당합니다. ) 세포 진입에 중요한 것으로 알려져 있습니다. 우리는 S1/S2 절단 부위에 인접한 에피토프에 대해 양성인 혈장이 스파이크의 푸린 매개 단백질 분해를 억제한다는 생화학적 증거를 보여줍니다.