Background Melanoma differentiation associated gene 5 (MDA5) and retinoic acid-inducible gene-I (RIG-I) selectively sense cytoplasmic viral RNA to induce an antiviral immune response. Infectious bronchitis virus (IBV) is one of the most important infectious agents in chickens, and in chicken cells, it can be recognized by MDA5 to activate interferon production. RIG-I is considered to be absent in chickens. However, the absence of RIG-I in chickens raises the question of whether this protein influences the antiviral immune response against IBV infection. Results Here, we showed that chicken cells transfected with domestic goose RIG-I (dgRIG-I) exhibited increased IFN-β activity after IBV infection. We also found that IBV can cleave MAVS, an adaptor protein downstream of RIG-I and MDA5 that acts as a platform for antiviral innate immunity at an early stage of infection. Conclusions Although chicken MDA5 (chMDA5) is functionally active during IBV infection, the absence of RIG-I may increase the susceptibility of chickens to IBV infection, and IBV may disrupt the activation of the host antiviral response through the cleavage of MAVS. Electronic supplementary material The online version of this article (doi: 10.1186/s12917-017-1253-7) contains supplementary material, which is available to authorized users.
【저자키워드】 MAVS, Infectious Bronchitis Virus, Melanoma differentiation associated gene 5, Retinoic acid-inducible gene-I, 【초록키워드】 Antiviral, Innate immunity, susceptibility, Infection, interferon, virus, Protein, cells, MDA5, cleavage, RIG-I, Viral RNA, early stage, platform, infectious agent, antiviral immune response, Activation, supplementary material, IFN-β, bronchitis, host antiviral response, IBV infection, downstream, IBV, Cell, raise, Result, exhibited, question, absence, cytoplasmic, induce, activate, influence, disrupt, cleave, transfected with, 【제목키워드】 Protein, MDA5, Melanoma, cleavage, signaling pathway, disrupt,