Summary We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the vsv-g gene. To prepare rcVSVs, we replace the vsv-g with a specific env -encoding gene, transfect cells with multiple plasmids for production of the genomic RNA and viral proteins, and rescue replication-competent viruses. Graphical abstract Highlights • Heterologous, viral envelope glycoproteins can be displayed on recombinant VSV • Recombinant VSVs allow study of the biology of viral entry of different viruses • Recombinant VSVs can be used to measure virus neutralization and as vaccines We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the vsv-g gene. To prepare rcVSVs, we replace the vsv-g with a specific env -encoding gene, transfect cells with multiple plasmids for production of the genomic RNA and viral proteins, and rescue replication-competent viruses.
【저자키워드】 immunology, Molecular biology, Cell-based Assays, Cell culture, 【초록키워드】 Vaccine, Infection, Viral proteins, virus, viral entry, VSV, HIV-1, Virus neutralization, glycoprotein, Heterologous, SARS-CoV-2 spike, recombinant, genomic RNA, Plasmid, Particles, Abstract, stomatitis, viral envelope, replication-competent viruses, Cell, can be used, expressing, displaying, not carry, 【제목키워드】 protocol, virus, glycoprotein, stomatitis, viral envelope, displaying,