Persisting alterations and unique immune signatures have been previously detected in the peripheral blood of convalescent plasma (CP) donors at approximately two months after initial SARS-CoV-2 infection. This article presents the results on the sequential analysis of 47 CP donors at a median time of eight months (range 7.5–8.5 months) post infection, as assessed by flow cytometry. Interestingly, our results show a significant variation of the relevant immune subset composition among CP donors. Regarding innate immunity, both non-classical monocytes, and CD11b- granulocytes had fully recovered at eight months post COVID-19 infection. Intermediate monocytes and natural killer (NK) cells had already been restored at the two-month evaluation and remained stable. Regarding adaptive immunity, the COVID-19-related skewed Th1 and Th2 cell polarization remained at the same levels as in two months. However, low levels of total B cells were detected even after eight months from infection. A persisting reduction of CD8+ Tregs and changes in the NKT cell compartment were also remarkable. CP donors present with a unique immune landscape at eight months post COVID-19 infection, which is characterized by the notable restoration of the components of innate immunity along with a persisting imprint of SARS-CoV-2 in cells of the adaptive immunity.
【저자키워드】 COVID-19, SARS-CoV-2, immune profiling, 8 months, convalescence plasma donors, immune restoration, 【초록키워드】 convalescent plasma, adaptive, Immunity, Innate immunity, SARS-COV-2 infection, Variation, Th1, Th2, Infection, flow cytometry, immune, Peripheral blood, Non-classical monocytes, B cell, COVID-19 infection, donors, Treg, Donor, CD11b, Analysis, granulocyte, reduction, natural killer, median time, alteration, component, CD8+, post infection, Cell, NKT, initial, immune signature, remained, eight, characterized, changes in, unique, restored, notable, subset, Intermediate monocyte, skewed, 【제목키워드】 Innate, alteration, peripheral, Month,