Surface disinfection is part of a larger mitigation strategy to prevent the spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus causing coronavirus disease-2019 (COVID-19). Research evaluating the time, nature, and extent of surface disinfection of replication-competent viruses is needed. We evaluated the efficacy of two disinfectants against a replication-competent SARS-CoV-2 surrogate on three common public surfaces. Vesicular stomatitis virus expressing green fluorescent protein (VSV-GFP) was our replication-competent SARS-CoV-2 surrogate. Disinfection occurred using Super Sani-Cloth Germicidal Disposable Wipes and Oxivir Tb spray per manufacturer instructions to test the efficacy at reducing the presence, viability, and later replication of VSV-GFP on stainless steel, laminate wood, and porcelain surfaces using standardized methods after recovery and toxicity testing. During the main trials, we placed 100 µL spots of VSV-GFP at viral titers of 10 8 , 10 7 , and 10 6 PFU/mL on each surface prior to disinfection. Trials were completed in triplicate and post-disinfection measurements on each surface were compared to the measurements of non-disinfected surfaces. Disinfectants were considered efficacious when ≥3-log 10 reduction in the number of infectious VSV-GFP virus units was observed on a given surface during all trials. Both disinfectants produced a ≥3.23-log 10 reduction in infectious VSV-GFP virus unit numbers, with all trials showing no viable, replication-competent VSV-GFP present on any tested surface. The two disinfectants eliminated the presence, viability, and later replication of VSV-GFP, our SARS-CoV-2 surrogate, on all surfaces. This information suggests that, if following manufacturer instructions, overcleaning surfaces with multiple disinfectant solutions may be unnecessary.
【저자키워드】 COVID-19, infection control, Infectious diseases, Virology, Disinfection, 【초록키워드】 SARS-CoV-2, Efficacy, coronavirus, Trial, Toxicity, virus, Replication, Spread, Protein, viability, surface, information, germicidal, trials, Coronavirus-2, vesicular stomatitis, acute respiratory syndrome, fluorescent, disinfectant, viral titer, replication-competent SARS-CoV-2, replication-competent virus, wipe, Prevent, VSV-GFP, produced, tested, occurred, evaluated, reducing, expressing, reduction in, eliminated, VSV-GFP virus, 【제목키워드】 SARS-CoV-2, surface, surrogate, disinfectant, common, vesicular,