CRISPR-Cas proteins appear not only as a genome editing tool but also as a promising diagnostic tool to detect nucleic acid targets, however, most of the CRISPR-based biosensors rely on labelled reporters for signal acquisition. Here, the authors develop a label-free CRISPR-Cas12a-based sensing platform by using supercoiled plasmid DNA as ratiometric reporters, detecting ssDNA targets with a limit of detection of around 100 fM, and applying it to detect various virus samples.
All Keywords
【저자키워드】 DNA, sensors, Bioanalytical chemistry,
【저자키워드】 DNA, sensors, Bioanalytical chemistry,