Pharmaceutical interventions are urgently needed to prevent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and transmission. As SARS-CoV-2 infects and spreads via the nasopharyngeal airways, we analyzed the antiviral effect of selected nasal and oral sprays on virus infection in vitro. Two nose sprays showed virucidal activity but were cytotoxic precluding further analysis in cell culture. One nasal and one mouth spray suppressed SARS-CoV-2 infection of TMPRSS2-expressing Vero E6 cells and primary differentiated human airway epithelial cultures. The antiviral activity in both sprays could be attributed to polyanionic ι- and κ-carrageenans. Thus, application of carrageenan-containing nasal and mouth sprays may reduce the risk of acquiring SARS-CoV-2 infection and may limit viral spread, warranting further clinical evaluation.
【저자키워드】 virus transmission, carrageenan, sulfated polysaccharides, virucidal, virus inhibition, TMPRSS2, 【초록키워드】 SARS-CoV-2, coronavirus, SARS-COV-2 infection, Infection, risk, Intervention, Transmission, nasal, in vitro, antiviral activity, severe acute respiratory syndrome Coronavirus, viral spread, Antiviral effect, Spread, Viral, clinical evaluation, nasopharyngeal, Cell culture, carrageenan, virus infection, respiratory, Vero E6 cell, Analysis, airways, Vero E6, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, cultures, pharmaceutical, cytotoxic, Pharmaceutical interventions, infect, Prevent, limit, selected, analyzed, suppressed, reduce, human airway epithelial, 【제목키워드】 Infection, nasal, airway, Culture, epithelial, inhibit SARS-CoV-2,