ABSTRACT Bacterial-viral interactions in saliva have been associated with morbidity and mortality for respiratory viruses such as influenza and SARS-CoV. However, such transkingdom relationships during SARS-CoV-2 infection are currently unknown. Here, we aimed to elucidate the relationship between saliva microbiota and SARS-CoV-2 in a cohort of newly hospitalized COVID-19 patients and controls. We used 16S rRNA sequencing to compare microbiome diversity and taxonomic composition between COVID-19 patients ( n = 53) and controls ( n = 59) and based on saliva SARS-CoV-2 viral load as measured using reverse transcription PCR (RT-PCR). The saliva microbiome did not differ markedly between COVID-19 patients and controls. However, we identified significant differential abundance of numerous taxa based on saliva SARS-CoV-2 viral load, including multiple species within Streptococcus and Prevotella . IMPORTANCE Alterations to the saliva microbiome based on SARS-CoV-2 viral load indicate potential biologically relevant bacterial-viral relationships which may affect clinical outcomes in COVID-19 disease.
【저자키워드】 COVID-19, SARS-CoV-2, Viral load, saliva microbiome, 【초록키워드】 Saliva, SARS-CoV, Influenza, SARS-COV-2 infection, Sequencing, RT-PCR, COVID-19 disease, Clinical outcome, 16S rRNA, Cohort, microbiota, respiratory virus, Microbiome, Control, morbidity and mortality, reverse transcription PCR, hospitalized COVID-19 patient, Interaction, Prevotella, COVID-19 patient, alteration, SARS-CoV-2 viral load, Streptococcus, Affect, controls, not differ, 【제목키워드】 oral, alteration, load, with COVID-19,