ABSTRACT The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed naïve sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.
【저자키워드】 COVID-19, SARS-CoV-2, coronavirus, swine, PIGS, zoonotic disease, infection models, 【초록키워드】 Efficacy, Vaccine, Mortality, antibody, SARS-COV-2 infection, susceptibility, Infection, Transmission, animal model, global pandemic, Consequences, viral replication, morbidity, Clinical signs, SARS-CoV-2 pathogenesis, marker, Evidence, cell lines, pig, naïve, cytopathic effects, cell line, carrier, responses, susceptible, effective, develop, unlikely, replicate, 【제목키워드】 Cell,