We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
All Keywords
【저자키워드】 COVID-19, coronavirus disease, severe acute respiratory syndrome coronavirus 2, viruses, respiratory infections, zoonoses, whole-genome sequencing, SARS-CoV-2, coronavirus, Genomics, High-throughput nucleotide sequencing, 【초록키워드】 protocol, Sequencing, Genome, virus, Laboratory, Sanger sequencing, multiplex, reverse transcription PCR, MiSeq, acute respiratory syndrome, full-length, 【제목키워드】 coronavirus 2, Rapid, respiratory, sensitive,
【저자키워드】 COVID-19, coronavirus disease, severe acute respiratory syndrome coronavirus 2, viruses, respiratory infections, zoonoses, whole-genome sequencing, SARS-CoV-2, coronavirus, Genomics, High-throughput nucleotide sequencing, 【초록키워드】 protocol, Sequencing, Genome, virus, Laboratory, Sanger sequencing, multiplex, reverse transcription PCR, MiSeq, acute respiratory syndrome, full-length, 【제목키워드】 coronavirus 2, Rapid, respiratory, sensitive,