Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 and is capable of human-to-human transmission and rapid global spread. The rapid emergence and global spread of SARS-CoV-2 has encouraged the establishment of a rapid, sensitive, and reliable viral detection and quantification methodology. Here, we present an alternative assay, termed immuno-plaque assay (iPA), which utilizes a combination of plaque assay and immunofluorescence techniques. We have extensively optimized the conditions for SARS-CoV-2 infection and demonstrated the great flexibility of iPA detection using several antibodies and dual-probing with two distinct epitope-specific antibodies. In addition, we showed that iPA could be utilized for ultra-high-throughput viral titration and neutralization assay within 24 h and is amenable to a 384-well format. These advantages will significantly accelerate SARS-CoV-2 research outcomes during this pandemic period.
【저자키워드】 SARS-CoV-2, Coronaviruses, immuno-plaque assay (iPA), viral quantification, 【초록키워드】 coronavirus disease, antibodies, pandemic, antibody, SARS-COV-2 infection, outcome, Spread, Neutralization assay, Viral detection, methodology, immunofluorescence, quantification, Coronavirus-2, Combination, Plaque assay, acute respiratory syndrome, SARS-CoV-2 research, human-to-human transmission, 384-well format, significantly, addition, condition, demonstrated, accelerate,