The humoral response through neutralizing antibodies (NAbs) is a key component of the immune response to COVID-19. However, the plaque reduction neutralization test (PRNT), the gold standard for determining NAbs, is technically demanding, time-consuming and requires BSL-3 conditions. Correlating the NAbs and total antibodies levels, assessed by generalized and automated serological tests, is crucial. Through a commercial surrogate virus neutralization test (sVNT), we aimed to evaluate the production of SARS-CoV-2 NAbs in a set of vaccinated healthcare workers and to correlate these NAbs with the SARS-CoV-2 IgG anti-S1, anti-RBD and anti-S2 serological titers. We found that 6 months after vaccination, only 74% maintain NAbs for the Wuhan strain/UK variant (V1) and 47% maintain NAbs for the South African and Brazil variants (V2). Through Spearman’s correlation, we found the following correlations between the percentage of inhibition of NAbs and the SARS-CoV-2 IgG II Quant (Abbott Laboratories, Chicago, IL, USA) and BioPlex 2200 SARS-CoV-2 IgG Panel (Bio-Rad, Hercules, CA, USA) immunoassays: rho = 0.87 (V1) and rho = 0.73 (V2) for anti-S1 assessed by Abbott assay; rho = 0.77 (V1) and rho = 0.72 (V2) for anti-S1, rho = 0.88 (V1) and rho = 0.82 (V2) for anti-RBD, and rho = 0.68 (V1) and rho = 0.60 (V2) for anti-S2 assessed by BioPlex assay ( p < 0.001 for all). In conclusion, we found a strong correlation between this fast, user-friendly, mobile and bio-safe sVNT and the serological immunoassays.
【저자키워드】 SARS-CoV-2, BNT162b2 vaccine, Neutralizing antibodies, surrogate virus neutralization test, serological immunoassays, 【초록키워드】 COVID-19, neutralizing antibody, Brazil, IgG, immune response, vaccination, variant, healthcare worker, immunoassays, virus neutralization test, African, Wuhan, automated, total antibody, USA, correlation, Chicago, serological, SARS-CoV-2 IgG, PRNT, NAb, anti-RBD, humoral, Abbott, gold standard, NAbs, sVNT, anti-S2, South, Abbott Laboratories, Bio-rad, Spearman’s correlation, plaque reduction neutralization, BSL-3, Hercules, evaluate, maintain, conditions, time-consuming, Panel, SARS-CoV-2 NAb, the SARS-CoV-2, 【제목키워드】 BNT162b2, titer, SARS-CoV-2 IgG, Anti-S1, Assessing,