Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.
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【저자키워드】 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Coronavirus disease 2019 (COVID-19), molecular diagnostics, real-time reverse transcriptase polymerase chain (RT-PCR), 【초록키워드】 COVID-19, public health, SARS-CoV-2, Coronavirus disease 2019, coronavirus, pandemic, protocol, Infectious disease, Rapid diagnostic test, CDC, outbreak, Taiwan, acute respiratory syndrome, gold standard, polymerase chain, real-time reverse transcriptase, caused, 【제목키워드】 COVID-19, CDC, optimization,
【저자키워드】 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Coronavirus disease 2019 (COVID-19), molecular diagnostics, real-time reverse transcriptase polymerase chain (RT-PCR), 【초록키워드】 COVID-19, public health, SARS-CoV-2, Coronavirus disease 2019, coronavirus, pandemic, protocol, Infectious disease, Rapid diagnostic test, CDC, outbreak, Taiwan, acute respiratory syndrome, gold standard, polymerase chain, real-time reverse transcriptase, caused, 【제목키워드】 COVID-19, CDC, optimization,