Comparison Between a Standard and SalivaDirect RNA Extraction Protocol for Molecular Diagnosis of SARS-CoV-2 Using Nasopharyngeal Swab and Saliva Clinical SamplesBioengineering and Biotechnology Published on 2021-03-292022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] 95% CI acquisition amplified binding clinical clinical samples Concordance COVID-19 COVID-19 pandemic diagnosis of COVID-19 extraction False negative genomic RNA individual involved lab Laboratory Local Molecular diagnosis Nasopharyngeal swab nasopharyngeal swab sample nucleic acid extraction oropharyngeal swab PCR processed produced Protein proteinase protocol reduce reduction reported RNA RNA extraction protocol Saliva Sample SARS-CoV-2 sensitivity Standard suspected patients Variation were assessed [DOI] 10.3389/fbioe.2021.638902 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Comparative effects of viral-transport-medium heat inactivation upon downstream SARS-CoV-2 detection in patient samples환자 샘플에서 다운스트림 SARS-CoV-2 검출에 대한 바이러스 수송 매체 열 비활성화의 비교 효과Comparative Study Published on 2021-03-182022-09-10 Journal: Journal of Medical Microbiology [Category] SARS, 진단, 치료기술, [키워드] Accuracy Affect alleviate alternatives approach arising CDC chain Chain Reaction clinically Combination Combined comparable Comparative Control correlated COVID-19 COVID-19 infection COVID-19 pandemic diagnosing diagnostic disease control downstream Effect Environmental impact evaluated extraction facilitate Healthcare provider healthcare providers heat inactivation heat-inactivation method infection control LightCycler 480 II Measures N1 and N2 National NHS outcome Patient performed polymerase chain reaction positive prevention probe protocol protocols provided purified RNA qRT-PCR qScript R 2 Rapid reaction reagent reagents reduce reducing reported required Resilience Result reverse transcription RNA extraction Roche RT-qPCR testing SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 genome SARS-CoV-2 virus sensitivity service supply chains Swab test result tested the SARS-CoV-2 virus Treatment Viral viral genomes viral inactivation viral transport media virus inactivation writing [DOI] 10.1099/jmm.0.001301 PMC 바로가기 [Article Type] Comparative Study
A comparison of SARS-CoV-2 wastewater concentration methods for environmental surveillance환경감시를 위한 SARS-CoV-2 폐수 농축법 비교Article Published on 2021-03-152022-09-11 Journal: The Science of the total environment [Category] SARS, 치료기술, [키워드] 95% CI analyzed Area Asymptomatic average Concentration diagnostic diagnostic testing Efficiency Environmental surveillance evaluate evaluated events extraction feasible feces filtration glycol highest human coronavirus human coronavirus OC43 include limitation lowest multiple method multiple methods OC43 Pathogens performed plant Polyethylene glycol Result RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 detection Seattle Sludge standard curve Surveillance the SARS-CoV-2 Total total RNA Treatment Ultrafiltration Variability Volume wastewater water while [DOI] 10.1016/j.scitotenv.2020.144215 PMC 바로가기 [Article Type] Article
Assessing the feasibility and effectiveness of household-pooled universal testing to control COVID-19 epidemicsCOVID-19 전염병을 통제하기 위한 가구 공동 만능 검사의 타당성과 효율성 평가Research Article Published on 2021-03-092022-09-12 Journal: PLoS Computational Biology [Category] SARS, 진단, [키워드] approach Assessing Combination Community Compliance Contact Contact tracing country COVID-19 COVID-19 epidemic determine Effectiveness effort Epidemic Epidemics epidemiological evaluate extraction facilitate feasibility feasible Health care Health care system health care systems individual infected individual Infection initial Isolation Mask mitigate Outbreaks PCR test PCR testing PCR tests positive positive PCR positive PCR test pre-symptomatic reduction required robustness SARS-CoV-2 Simulation social distancing suggested tested the epidemic Transmission transmitted virus [DOI] 10.1371/journal.pcbi.1008688 PMC 바로가기 [Article Type] Research Article
Extraction-free protocol combining proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCRRT-qPCR에 의한 SARS-CoV-2 검출을 위한 proteinase K와 열 비활성화를 결합한 추출 없는 프로토콜Research Article Published on 2021-02-262022-08-13 Journal: PLoS ONE [Category] Biochemistry, MERS, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis clinical sample clinical samples coronavirus country Device Early detection extraction extraction step extraction-free RT-qPCR followed by food heat inactivation heat-inactivation IMPROVE include Medical devices multiplexing detection kit Nasopharyngeal swab nasopharyngeal swabs nasopharyngeal swabs specimens ORF1ab PK+HID PK+HID method positive proteinase Proteinase K protocol purified viral RNA Real-time reverse transcription PCR respiratory reverse transcription PCR RNA extraction robust RT-qPCR saline solution SARS-CoV-2 SARS-CoV-2 detection sensitivity severe acute respiratory syndrome Coronavirus specificity time-consuming Treatment variety Viral viral gene [DOI] 10.1371/journal.pone.0247792 PMC 바로가기 [Article Type] Research Article
A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater SamplesFull Paper Published on 2021-02-222022-10-31 Journal: Global Challenges [Category] COVID-19, [키워드] biohazard clinical Community contagious COVID‐19 Diagnosis equipment evaluated extraction inactivated infectious sample Influenza influenza viruses Isolation Laboratory lysed Magnetic nanoparticles membrane nanoparticle nucleic acid Nucleic acid purification pathogen pathogen detection Pathogens Patient reagent reagents reducing respiratory syncytial virus risk RNA RNA extraction Safe Sample SARS‐CoV‐2 SARS‐CoV‐2 shown Surveillance Trizol Viral RNA wastewater wastewater surveillance widespread [DOI] 10.1002/gch2.202000068 PMC 바로가기 [Article Type] Full Paper
A Deep Learning-Based Camera Approach for Vital Sign Monitoring Using Thermography Images for ICU PatientsICU 환자를 위한 열화상 영상을 사용한 생체 신호 모니터링을 위한 딥 러닝 기반 카메라 접근 방식Article Published on 2021-02-212022-08-13 Journal: Sensors (Basel, Switzerland) [Category] 임상, [키워드] addition Algorithm Algorithms applied bpm camera camera-based vital sign measurement Chest clinical trial Combination conducted COVID-19 pandemic deep deep learning detect determine extraction Fever fevers Frequency GPU head ICU ICU monitoring Image increasingly infections infrared frame infrared frames infrared thermography intensive care intensive care unit intersection over union investigated IRT mean absolute error mean average precision medical examinations module MONITOR monitoring object detection object detector optical flow optical flow algorithm Patient Region respiratory rate SIGN skin temperature temperature test dataset the patient thermography union validation Vital vital sign vital signs was performed was used YOLOv4 YOLOv4-Tiny [DOI] 10.3390/s21041495 PMC 바로가기 [Article Type] Article
Knowledge, attitude, practice towards COVID-19 pandemic and its prevalence among hospital visitors at Ataye district hospital, Northeast Ethiopia에티오피아 북동부 아타에(Atay) 지역 병원의 코로나19 팬데믹에 대한 지식, 태도, 관행 및 병원 방문자의 유병률Clinical Trial Published on 2021-02-192022-08-13 Journal: PLoS ONE [Category] MERS, 임상, 치료기술, [키워드] 2019-nCoV 95% CI age alcohol Analysis analyzed association Attitude candidate Chain Reaction Characteristics Community conducted confidence interval coronavirus coronavirus disease coronavirus disease 19 country COVID-19 COVID-19 pandemic cross demonstrated determine equipment extraction Factors family Family size feasible female gender fluorescent Good Health healthcare hospital Human resources information International Intervention KAP knowledge Laboratory less Limited magnitude majority Misconception Misconceptions moderate molecular multivariable analysis multivariable logistic regression Novel coronavirus nucleic acid P -value P-value pandemic polymerase chain polymerase chain reaction positive attitude Prevalence Prevention and control priority processed professional proportion public health emergency questionnaire random recruited resource Result retained Risk factors RT-PCR socio-demographic specimen statistically significant study participant Study participants Treatment treatment of COVID-19 variable Viral viral nucleic acid viral spread virus was collected was used [DOI] 10.1371/journal.pone.0246154 PMC 바로가기 [Article Type] Clinical Trial
Pooled testing with replication as a mass testing strategy for the COVID-19 pandemicsCOVID-19 전염병에 대한 대규모 테스트 전략으로 복제를 사용한 통합 테스트Article Published on 2021-02-102022-09-01 Journal: Scientific Reports [Category] MERS, 진단, 치료기술, [키워드] Applied mathematics approach average conjunction COVID-19 COVID-19 pandemic detect extraction filament group Infection Mild nasopharyngeal swabs Oropharyngeal outbreak Pandemics Patient present reactants Real-time PCR reduce reduction Replication RNA Sequencing Swab swabs tested viral infection virus [DOI] 10.1038/s41598-021-83104-4 PMC 바로가기 [Article Type] Article
[Strategy for planning and execution of a large-scale SARS-CoV-2 seroprevalence study on teaching hospital workers during a pandemic situation][팬데믹 상황에서 병원 종사자 교육을 위한 대규모 SARS-CoV-2 혈청 유병률 연구 계획 및 실행을 위한 전략]Article Published on 2021-01-012022-09-12 Journal: Journal of healthcare quality research [Category] MERS, SARS, 진단, [키워드] analysed assigned candidate carried Compliance Coverage COVID-19 Descriptive analysis English extraction Health hospital involved Organisation and administration Organización y administración pandemic participated professional reliability SARS-CoV-2 SARS-CoV-2 seroprevalence serología serology Spain Spanish [DOI] 10.1016/j.jhqr.2020.11.003 PMC 바로가기 [Article Type] Article