Computational Analysis of Targeting SARS-CoV-2, Viral Entry Proteins ACE2 and TMPRSS2, and Interferon Genes by Host MicroRNAsCommunication Published on 2020-11-162022-10-31 Journal: Genes [Category] COVID-19, SARS, [키워드] 3′-UTR 5′-UTR ACE2 acute respiratory syndrome Algorithm binding site biological plausibility Biomarkers cell lines Combination coronavirus coronavirus disease COVID-19 Critical dataset global pandemic health care systems host miRNA identify IFN IFN-α infected cells interactions Local mechanism microRNA miR-29a miRNA miRNA profiling miRNAs mRNA transcript ORF potential therapeutic target predict predicted proteins ACE2 provide Rapid regulated Regulatory responsible RNA RNA viruses SARS-CoV-2 SARS-CoV-2-infected cell Spread targeting the interferon the SARS-CoV-2 genome TMPRSS2 upstream viral entry viral pathogenesis virus [DOI] 10.3390/genes11111354 PMC 바로가기 [Article Type] Communication
Repurposing Sigma-1 Receptor Ligands for COVID-19 Therapy?Pharmacology Published on 2020-11-092022-10-31 Journal: Frontiers in Pharmacology [Category] COVID-19, [키워드] activate acute respiratory syndrome adaptive Anti-viral Antiviral Antiviral compound antiviral drug approach approaches approved drug benefit cause chaperone clinical development clinical trials coronavirus Course COVID-19 COVID-19 pandemic COVID-19 patients CoVs Drug repurposing drugs Endoplasmic reticulum ER membranes ER stress expected facilitate Health host cell host cell response host protein identify in vitro Infection infections interact Interaction interfere Intervention Ligand mechanism Medicine membrane-bound modulation occur pathway patients potential mechanism Prevent professional protective immune response Proteins provide receptor reduce regulate Repurposed drugs repurposing SARS-CoV-2 SARS-CoV-2 protein sigma-1 receptor Stress targeting the disease therapeutic therapy treat unique upstream viral replication [DOI] 10.3389/fphar.2020.582310 PMC 바로가기 [Article Type] Pharmacology
Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical EvaluationArticle Published on 2020-11-042022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome added Antigen approaches binding bioreactor Cell cells conformational coronavirus COVID-19 COVID-19 therapeutics COVID-19 vaccine Culture cultures demonstrated downstream efficient Epitopes evaluated exhibited expression functional HEK293SF suspension cells Improvements Infection mechanism media monoclonal antibody motif Neutralizing antibodies Plasmid Production Protein Rapid RBD Research resulting SARS-CoV-2 SARS-CoV-2 Spike Receptor Binding Domain sialic acid spike subunit supernatants Support the RBD protein the Spike Transient transient transfection upstream Vaccine vaccine bioprocess virus [DOI] 10.3390/vaccines8040654 PMC 바로가기 [Article Type] Article
Complement C3 vs C5 inhibition in severe COVID-19: Early clinical findings reveal differential biological efficacyResearch article Published on 2020-11-012022-10-05 Journal: Clinical immunology (Orlando, Fla.) [Category] SARS, 바이오마커, [키워드] abrogate Activation acute respiratory distress AMY-101 anti-inflammatory response ARDS Biomarkers blockade C-reactive protein C3 inhibition C3a C5 blockade C5a candidate drug clinical evidence Clinical finding Cohort complement consequence COVID-19 COVID-19 hyper-inflammation COVID-19 patient COVID-19 therapeutics detrimental drug candidate Drug efficacy eculizumab effector Efficacy elicit exploratory study faster hyper-inflammation IL-6 levels immunopathology immunothrombosis implicated independent indicate inhibitor inhibitory Intervention LDH Lung function lymphocyte monoclonal antibody multi-organ failure NET neutrophil counts Neutrophil extracellular trap offer outcome pathogenic pathway reflected robust SARS-COV-2 infection SC5b-9 serum severe COVID-19 patients Support syndrome therapeutic Thromboinflammation thrombotic trials upstream [DOI] 10.1016/j.clim.2020.108598 [Article Type] Research article
Direct activation of the alternative complement pathway by SARS-CoV-2 spike proteins is blocked by factor D inhibitionArticle Published on 2020-10-292022-10-06 Journal: Blood [Category] SARS, 진단, [키워드] activate Activation acute respiratory syndrome Alternative pathway animal antibody APC Atypical blocked C3c catastrophic cells clinical manifestation complement Complement pathway contagious coronavirus COVID-19 Direct disease end-organ end-organ damage endothelial damage Evidence explain Human Hypothesis in vitro inactivation Injury killing mechanism mice mitigate Myocardial infarction Prevent renal respiratory virus SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 spike proteins Spike protein stroke subunit supernatant syndrome target cells the N protein the SARS-CoV-2 thrombosis Trigger upstream uremic syndrome [DOI] 10.1182/blood.2020008248 PMC 바로가기 [Article Type] Article
Single-Cell Transcriptome Analysis Highlights a Role for Neutrophils and Inflammatory Macrophages in the Pathogenesis of Severe COVID-19Article Published on 2020-10-292022-10-31 Journal: Cells [Category] COVID-19, MERS, SARS, [키워드] activated Algorithm Analysis APOC1 ApoE BAL BCL2A1 Bronchoalveolar lavage Bronchoalveolar lavage (BAL) C1QB canonical category CCL2 CCL3 CCL5 CCL8 CCR1 Cell cellular chemotactic chemotaxis circulation Clustering Cohort complex coronavirus disease correlation COVID-19 CXCL8 Cytokine storm dataset diagnostic disease severity downregulated EPSTI1 FABP4 FIVE FPR1 functional gene signature gene signatures hallmark healthy subject healthy subjects ICGS2 identify IFI27 IFI30 Ifi44 IFI44L IFI6 IFIT1 IFIT3 IFITM1 IFITM2 IFITM3 IFNg IL17 IL1A IL1B IL1RN immune Immune cell Inflammatory inflammatory macrophages Inflammatory response interstitial iterative KLF6 Macrophage MALAT1 Mild mononuclear cell MX1 myeloid cells NEAT1 neutrophil Neutrophils NFkB OAS1 OAS2 OAS3 OASL pathway Patient patients PBMCs Peripheral blood phagocytes phagocytosis PLSCR1 PRL reduction reflected role S100A8 SARS-CoV-2 SAT1 several gene severe COVID-19 severity Signaling Single Cell SPP1 STAT3 subset suppressed systemically TGM2 therapeutic interventions TLR7 TLR9 TNF TYMP UMAP upstream with COVID-19 [DOI] 10.3390/cells9112374 PMC 바로가기 [Article Type] Article
Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virusResearch Article Published on 2020-10-282022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, MERS, [키워드] 3′-UTR accessory gene assessment avian coronavirus canonical complementary coronavirus deep demonstrated discontinuous transcription Express full-length full-length protein Genome had no IBV identify in vitro indicate Infectious Bronchitis Virus lack leader sequence mRNA Multiple N gene occur ORF produced Protein RNA RNA synthesis Sequencing analysis sgmRNA sgmRNAs single nucleotide strain sub-genomic mRNA thought transcribed Transcription transcription regulatory sequence TRS-B TRS-L TRSs upstream UTR viral replication [DOI] 10.1099/jgv.0.001474 PMC 바로가기 [Article Type] Research Article
Point mutation bias in SARS-CoV-2 variants results in increased ability to stimulate inflammatory responsesArticle Published on 2020-10-202022-10-04 Journal: Scientific Reports [Category] MERS, SARS, 변종, [키워드] acute respiratory syndrome Affect cell line coronavirus Cytokines cytosine downstream enzyme Enzymes Factor gene mutation genomes guanine Host IL-6 immunology Impact induce Infection Inflammatory cytokine Inflammatory response mutated Mutation mutations Pathogenesis Point Point mutation prevalent Proofreading resulting RNA RNA viruses SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sequence severe pneumonia specificity ssRNA stimulate TNF-α upstream uracil variants of SARS-CoV-2 virus worldwide pandemic Wuhan [DOI] 10.1038/s41598-020-74843-x PMC 바로가기 [Article Type] Article
Naturally occurring SARS-CoV-2 gene deletions close to the spike S1/S2 cleavage site in the viral quasispecies of COVID19 patientsResearch Article Published on 2020-09-022022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] accumulate ACE2 receptor binding Burn circulation Complete COVID19 patient Deletion deletions described Diversity facilitate frameshift generate Genome host cell Infection insertions Mild Mutation rates natural selection Naturally Neutralizing antibodies NGS other coronaviruses Patient Protein Quasispecies Recombination reducing released respiratory virus S1 protein S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 S gene SARS-CoV-2 spike severe COVID-19 severe COVID-19 patients severity stop codon tissue damage Transmission Treatment upstream Vaccine development viral particle viral quasispecy virus [DOI] 10.1080/22221751.2020.1806735 PMC 바로가기 [Article Type] Research Article
Confronting the Upstream Causes of COVID-19 and Other Epidemics to FollowV. The Need to Redefine the Response to the COVID-19 Pandemic Published on 2020-08-032022-10-30 Journal: International journal of health services : plannin [Category] Coronavirus, MERS, [키워드] Agriculture capitalism cause causes conflict COVID-19 COVID-19 pandemic Diseases downstream Effect Epidemic Evidence Health Organization impacted International morbidity and mortality National organization Other pandemic Pandemics philanthropy Prevent public health shown suffered Support upstream upstream causes viral epidemics [DOI] 10.1177/0020731420946612 PMC 바로가기 [Article Type] V. The Need to Redefine the Response to the COVID-19 Pandemic