Development and Evaluation of a Molecular Hepatitis A Virus Assay for Serum and Stool SpecimensArticle Published on 2022-01-152023-05-23 Journal: Viruses [Category] A형 간염, Fulltext, [키워드] clinical diagnostics Real-time PCR Viral hepatitis [DOI] 10.3390/v14010159 PMC 바로가기 [Article Type] Article
Identification of Hotspot Mutations in the N Gene of SARS-CoV-2 in Russian Clinical Samples That May Affect the Detection by Reverse Transcription-PCRArticle Published on 2022-01-082022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] Affect affected clinical clinical sample detect detection diagnostic diagnostics E gene Evolution genetic variability highlight identification majority Mutation mutations N gene nonsynonymous mutation not affect PCR assays phosphorylated polymorphism positive Protein Real-time PCR Region RT-PCR RT-PCR assay Sample SARS-CoV-2 SARS-CoV-2 pandemic selected sensitive sensitivity target target gene target region the SARS-CoV-2 virus was performed [DOI] 10.3390/diagnostics12010147 PMC 바로가기 [Article Type] Article
Saliva Quantification of SARS-CoV-2 in Real-Time PCR From Asymptomatic or Mild COVID-19 AdultsMicrobiology Published on 2022-01-032022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] age analyzed Asymptomatic charge Clinical characteristics clinical symptom co-morbidity COVID-19 COVID-19 positive patient demographics diagnosed discharge disease droplet French groups had no headache in viral Infectivity initial viral load IQR log majority median Mild nasal onset of symptom Patient PCR quantification Quantitative Real-time PCR Real-time reverse transcription PCR recruited reduction Saliva salivary SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA viral SARS-CoV-2 transmission Sex spread of COVID-19 statistically significant difference suggested Symptom Symptoms transmitted Viral load virus isolation [DOI] 10.3389/fmicb.2021.786042 PMC 바로가기 [Article Type] Microbiology
Abnormal Transcript Levels of Cytokines Among Iranian COVID-19 Patients이란 COVID-19 환자 중 사이토카인의 비정상적인 전사 수준Article Published on 2022-01-012022-09-11 Journal: Journal of molecular neuroscience : MN [Category] 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 age AUC AUC value Biomarker C-reactive protein caused Clinical data coding gene coding genes collected controls coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 patient COVID-19 patients CRP cytokine Cytokine storm Cytokines diagnostic differentiation disease disease severity disorder erythrocyte sedimentation erythrocyte sedimentation rate Evidence expression expression level expression levels expression of gene female form Gender healthy control healthy controls hospital ICU admission IFN-g IL-17 IL-1B IL-2 IL-4 IL-6 IL-8 Level male molecular mechanism nine not different p value pandemic Pathogenesis Patient patients Protein provide ranged reached Real-time PCR researcher sedimentation rate sensitivity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significantly higher specificity Support TGF-B TNF-a transcript level transcript levels [DOI] 10.1007/s12031-021-01941-4 PMC 바로가기 [Article Type] Article
A SARS-CoV-2 nucleocapsid ELISA represents a low-cost alternative to lateral flow testing for community screening in LMI countriesSARS-CoV-2 뉴클레오캡시드 ELISA는 LMI 국가에서 지역사회 스크리닝을 위한 측면 흐름 테스트에 대한 저렴한 대안을 나타냅니다.Article Published on 2022-01-012022-09-11 Journal: The Journal of Infection [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] Antigen applied Asymptomatic Care Community controlling country cut-off Diagnosis diagnostic driven by effective ELISA ELISA assay Health Health care help hospitalised patient identify individual individuals lateral flow limit of detection nose nose and throat nucleocapsid Patient patients positive pre-symptomatic provide Real-time PCR reported required resources Result rising SARS-CoV-2 SARS-CoV-2 nucleocapsid sensitivity Sensitivity and specificity specificity Spread Sputum sample sputum samples swab sample Test tested Transmission Trial variety virus were excluded [DOI] 10.1016/j.jinf.2021.08.049 PMC 바로가기 [Article Type] Article
Evaluating sensitivity and specificity of the Biomeme Franklin™ three9 real-time PCR device and SARS-CoV-2 go-strips assay using clinical samplesBiomeme Franklin™ three9 real-time PCR 장치 및 SARS-CoV-2 go-strip assay의 임상 시료를 이용한 민감도 및 특이도 평가Article Published on 2022-01-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 치료기술, 치료제, [키워드] Austere environment Chain Reaction clinical sample Concentration COVID19 Cross reactivity cross-reactivity demonstrated detect diagnostic testing effective evaluated Evaluating examined Panel Pathogens PCR platform polymerase chain reaction ranged Real-time PCR respiratory Respiratory pathogens SARS-CoV-2 SARS-CoV-2 detection sensitivity Sensitivity and specificity significantly lower statistically target tested the SARS-CoV-2 viral concentrations [DOI] 10.1016/j.jcv.2021.105046 PMC 바로가기 [Article Type] Article
Validation and deployment of a direct saliva real-time RT-PCR test on pooled samples for COVID-19 surveillance testingCOVID-19 감시 테스트를 위해 수집된 샘플에 대한 직접 타액 실시간 RT-PCR 테스트의 검증 및 배포Research Article Published on 2021-12-302022-09-12 Journal: PLoS ONE [Category] 진단, [키워드] Abbott amplification Asymptomatic binary logistic regression analysis confirmatory tests COVID-19 Ct threshold Ct values diagnostic effective Evidence evidence of indicate Logistic regression analysis nasal swab oropharyngeal swabs participant Participants PCR PCR test positive Real-time PCR real-time reverse transcriptase real-time RT-PCR test receiving reverse transcriptase RT-PCR testing Saliva saliva samples SARS-CoV-2 SARS-CoV-2 surveillance Surveillance surveillance testing tested validation [DOI] 10.1371/journal.pone.0261956 PMC 바로가기 [Article Type] Research Article
Propagation of a hospital-associated cluster of COVID-19 in MalaysiaResearch Published on 2021-12-092022-10-28 Journal: BMC Infectious Diseases [Category] COVID-19, SARS, [키워드] Analysis approach Cluster Community complement Compliance conducted COVID-19 COVID-19 cases COVID-19 pandemic criteria demonstrated Diagnosis disease transmission effective Epidemic epidemiological Epidemiological investigation event examined excluded healthcare Healthcare system healthcare worker hospital identify imperative implementation increase Incubation period index case infection control Infection prevention initial Malaysia management measure Measures median morbidity and mortality Multidisciplinary nine outbreak Outbreaks patients positive Propagation Real-time PCR Result risk SARS-CoV-2 Sequencing Sequencing analysis subset suggested supplementary material Surveillance Symptom symptomatic the disease Transmission university whole genome Whole genome sequencing with COVID-19 [DOI] 10.1186/s12879-021-06894-y PMC 바로가기 [Article Type] Research
Prevalence, co-infection and seasonality of fecal enteropathogens from diarrheic cats in the Republic of Korea (2016–2019): a retrospective studyResearch Published on 2021-12-012022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] accounted addition age Bacterial infection Campylobacter caused Clinical characteristics Clinical features Clostridium Co-infection collected common clinical symptom coronavirus diarrhea distribution fecal feline Foetus Infection infection rate infections investigated pathogen Patient peaked positive predominant Prevalence Real-time PCR required Result Retrospective study viral infection [DOI] 10.1186/s12917-021-03075-6 PMC 바로가기 [Article Type] Research
Development of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus AntigenArticle Published on 2021-11-302022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] antibody Antigen antigen detection clinical sample Concentration detect detection Detection limit development diarrhea ELISA enzyme-linked immunosorbent Fecal sample inactivated Inactivated Vaccines Infection intestinal intestinal and fecal samples monoclonal antibody N protein Newborn novel Porcine deltacoronavirus Quantitative quantitative ELISA rabbit polyclonal antibody Real-time PCR remained RT-qPCR Sandwich screened sensitivity specificity TCID transcriptase Vaccine vaccine evaluation Viral antigen virus virus titer [DOI] 10.3390/v13122403 PMC 바로가기 [Article Type] Article