Accurate serology for SARS-CoV-2 and common human coronaviruses using a multiplex approachResearch Article Published on 2020-09-082022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] antibody approach circulating clinical evaluation coronavirus COVID-19 COVID-19 cohort diagnostics epidemiological evaluate evaluated heterogeneous human coronavirus human coronaviruses identify immune profiling immunoassay micro-array multiplex N antigen Patient Prevalence Protein provided public health Quantitative Research question responses SARS-CoV-2 SARS-CoV-2 antibody responses SARS-CoV-2 IgG antibody SARS-CoV-2 pandemic SARS-CoV-2 S1 sensitivity Serological assay serology Severity of disease specificity subset Support Symptom [DOI] 10.1080/22221751.2020.1813636 PMC 바로가기 [Article Type] Research Article
A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnosticsArticle Published on 2020-09-082022-10-04 Journal: Nature Communications [Category] SARS, 진단, 치료제, [키워드] approach Assay systems automated boost bottlenecks Community creating dependent on develop diagnostic diagnostics High-throughput screening Infectious diseases isothermal amplification lab London NHS Patient per day platform RNA extraction RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 testing scaled shown Synthetic biology [DOI] 10.1038/s41467-020-18130-3 PMC 바로가기 [Article Type] Article
COVID-19: An Emerging Threat to Antibiotic Stewardship in the Emergency DepartmentEndemic Infections Published on 2020-09-072022-10-28 Journal: Western Journal of Emergency Medicine [Category] COVID-19, MERS, [키워드] antibiotic approach Biomarker Care Combination COVID-19 COVID-19 patient demonstrated Department diagnostics Effect effective Efficacy effort Emergency exacerbating host response implementation Intervention lack mitigate pandemic Pathways Patient patient safety public health representing Research threat viral pathogens while [DOI] 10.5811/westjem.2020.7.48848 PMC 바로가기 [Article Type] Endemic Infections
Quantifying antibody kinetics and RNA detection during early-phase SARS-CoV-2 infection by time since symptom onsetResearch Article Published on 2020-09-072022-10-31 Journal: eLife [Category] COVID-19, SARS, [키워드] antibody antibody kinetics assays biological patterns COVID-19 datapoint decrease demographics detectable Detection probability diagnostics disease severity Evidence highest Human IgG IgG and IgM IgM individual Laboratory Lower respiratory tract mathematical Meta-analysis occur post-symptom onset Probability protocol public health RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 transmission Seroconversion serological survey significantly study group symptom onset understanding Variation Viral RNA [DOI] 10.7554/eLife.60122 PMC 바로가기 [Article Type] Research Article
Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNAEditor's Choice Published on 2020-09-052022-10-29 Journal: Journal of Medical Microbiology [Category] COVID-19, SARS, [키워드] alleviate average bypassing CDC clinical sample Combination conducted COVID-19 detect diagnostic diagnostics Dilution E-gene enzyme evaluated FAM FDA implementation intervals isothermal amplification Laboratory medium Nasopharyngeal swab nasopharyngeal swabs nucleocapsid primer required resource Result reverse transcription RNA extraction kit RNA purification RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA SARS-CoV-2 virus screened Sensitivity and specificity specimen TCID the SARS-CoV-2 thermocycler thermocyclers Transport tube universal transport media UTM validation virus detection [DOI] 10.1099/jmm.0.001238 PMC 바로가기 [Article Type] Editor's Choice
Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike proteinArticle Published on 2020-09-042022-10-04 Journal: NPJ Vaccines [Category] 진단, [키워드] Alter Analysis antibody antibody-based therapy B-cell epitope China diagnostics epitope flexible glycosylation pattern heterogeneous homogeneous identify Impact inaccessible N-glycosylation outbreak pandemic proportions Protein Receptor binding domain resulting S-protein S2 subunit SARS-CoV-2 spike spread to target the S1 subunit the Spike trimeric Vaccine Vaccine development Vaccines were used [DOI] 10.1038/s41541-020-00237-9 PMC 바로가기 [Article Type] Article
The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike ProteinLetter Published on 2020-09-042022-10-31 Journal: The journal of physical chemistry letters [Category] COVID-19, SARS, [키워드] antibody antibody binding site approach binding Combination complex conformational Consequences detect develop diagnostics Dynamics effort epitope Epitopes functional glycosylated Health Host identify Infection involved knowledge nanobodies parameter prediction PROTECT required SARS-CoV-2 simple Simulation spike Spike protein Structure [DOI] 10.1021/acs.jpclett.0c02341 PMC 바로가기 [Article Type] Letter
Collaborative networks enable the rapid establishment of serological assays for SARS-CoV-2 during nationwide lockdown in New ZealandMicrobiology Published on 2020-09-032022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] antibody Antigen binding calculated clinician Cohort Collaborative effort Concordance country COVID-19 COVID-19 pandemic COVID-19 patient Critical cut-off detect determine diagnostic reagent diagnostics effective vaccine ELISA hospital IgG ELISA immune response immunoassay Infection Interaction International investigated lockdown neutralising Neutralising Antibodies neutralising antibody New notable one individual overlap Patient PCR-confirmed COVID-19 patient PCR-negative positive produced quantified RBD reagent Receptor binding domain respiratory infection respiratory symptom Result SARS-CoV-2 SARS-CoV-2 patient Scientific community sera Serological assay serology seropositive serum antibody specificity Spike protein sVNT Symptom symptom onset symptomatic Transmission Travel utility viral neutralisation was used with COVID-19 [DOI] 10.7717/peerj.9863 PMC 바로가기 [Article Type] Microbiology
SARS-CoV-2/COVID-19 and advances in developing potential therapeutics and vaccines to counter this emerging pandemicReview Published on 2020-09-022022-10-28 Journal: Annals of Clinical Microbiology and Antimicrobials [Category] COVID-19, SARS, [키워드] affecting antibody antiviral drugs bats catastrophic China city conducted contagious coronavirus disease Coronaviruses country COVID-19 CoVs deaths develop Diagnosis diagnostics drugs Dyspnea effective Evolution Extensive Fever Genome genus Betacoronavirus globe help Immunotherapeutics Infection MERS-CoV molecular molecular basis Novel coronavirus pandemic pandemic virus Phylogenetic analysis Pneumonia Research RNA virus SARS-CoV SARS-CoV-2 SARS-CoV-2 virus severely infected patient Spread Symptom the disease the SARS-CoV-2 therapeutic therapeutic option Therapeutics turn Vaccine Vaccine development Vaccines virus Wuhan [DOI] 10.1186/s12941-020-00384-w PMC 바로가기 [Article Type] Review
Evolution of Early SARS-CoV-2 and Cross-Coronavirus ImmunityResearch Article Published on 2020-09-022022-10-30 Journal: mSphere [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Antibody Response asymptomatic infection binding caused changes in Cohort coronavirus correlation CoV CoVs Critical cross-reactivity detect determine develop diagnose diagnostic diagnostics disease disease manifestation Evidence Evolution Evolution of SARS-CoV-2 example explain Fc-receptor binding functional heterogeneity Heterologous HKU1 humoral immune response Humoral immunity hypothese immune response Immunity in viral individual Infection Influenza involved management Mild NL63 Novel coronavirus other coronavirus outcome prevalent RBDs Receptor binding Receptor binding domain respiratory infection respiratory infections Respiratory pathogens respiratory syncytial virus respiratory viruses response responses SARS-coronavirus SARS-CoV-2 severe disease Spread Symptom targets the SARS-CoV-2 Vaccine development Vaccine-induced immunity viral infection viruses [DOI] 10.1128/mSphere.00622-20 PMC 바로가기 [Article Type] Research Article