Zn 2+ Inhibits Coronavirus and Arterivirus RNA Polymerase Activity In Vitro and Zinc Ionophores Block the Replication of These Viruses in Cell CultureResearch Article Published on 2010-11-042022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] activity Antiviral arteritis arterivirus Author binding block both viruses catalyzed Cell Cell culture Combination complex complexes Concentration described divalent cation E. coli eliminating enzyme equine found function host proteins impair in vitro activity in vitro study include Increased Increasing Infected Influenza virus inhibit inhibited inhibitory effect Ionophore molecular nidovirus Nsp12 Nsp9 Pathogens plasma Poliovirus polymerases polyprotein polyproteins Proteolytic processing protocol provide purified RdRP RdRps reduced related Replication replication complex replicative Research reversed RNA RNA synthesis RNA viruses RNA-dependent RNA polymerase RTC SARS-coronavirus SARS-CoV target Transcription Transport variety Viral RNA virus Zinc Zn [DOI] 10.1371/journal.ppat.1001176 PMC 바로가기 [Article Type] Research Article
Prevalence and Phylogeny of Coronaviruses in Wild Birds from the Bering Strait Area (Beringia)Research Article Published on 2010-10-292022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Analysis Area Bering Strait Beringia Bird bronchitis cause collected costly CoV CoV infection CoVs detected Epidemiology examined Frequency Genetic Health host range Human IBV investigated Mild Novel coronavirus pathogenic performed phylogenetic analyses related reservoir respiratory viral disease RT-PCR SARS-coronavirus severe disease Spread statistical virus widespread [DOI] 10.1371/journal.pone.0013640 PMC 바로가기 [Article Type] Research Article
Bat Coronaviruses and Experimental Infection of Bats, the PhilippinesResearch Published on 2010-08-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, MERS, [키워드] Administered After Analysis bat bat coronavirus bats Clinical sign coronavirus CoV CoVs Cynopterus detect Experimental infection found fruit genus Alphacoronavirus genus Betacoronavirus group identity Infected Infection intestine investigated Laboratory Pathogenesis Prevalence Research RNA RNA-dependent RNA polymerase Rousettus SARS sequence sequence identity Severe acute respiratory syndrome tested the Philippines virus virus replication virus RNA viruses zoonoses [DOI] 10.3201/eid1608.100208 PMC 바로가기 [Article Type] Research
Mixed infections with Chlamydia and porcine epidemic diarrhea virus – a new in vitro model of chlamydial persistenceResearch Article Published on 2010-07-272022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] Alter appear Cell cells Chlamydia Chlamydiaceae Chlamydiae chronic disease Co-infection Culture detected developmental elementary body epidemic diarrhea evidenced experiment experiments heterogenous homogenous hpi Human Hypothesis immunofluorescence in vitro in vitro model Inclusion induce Infection infections investigated mixed infection NO observation PEDV persistence produced Re-infection Result TEM transmission electron microscopy Vero Cell virus [DOI] 10.1186/1471-2180-10-201 PMC 바로가기 [Article Type] Research Article
The relationship between antibody status to bovine corona virus and bovine respiratory syncytial virus and disease incidence, reproduction and herd characteristics in dairy herdsResearch Published on 2010-06-042022-10-28 Journal: Acta Veterinaria Scandinavica [Category] Coronavirus, Fulltext, MERS, [키워드] Affect analysed analyzed antibody antibody status association BCV both virus bovine Characteristics circulating collected compared cow disease incidence enzyme-linked immunosorbent assays evaluate the effect FIVE Health Herd Immunoglobulin Infection Infection transmission information Local mapped median negative parameter positive primiparous proportion reproductive respiratory syncytial virus Result somatic cell count Spread statistically significant Swedish Transmission virus [DOI] 10.1186/1751-0147-52-37 PMC 바로가기 [Article Type] Research
Infidelity of SARS-CoV Nsp14-Exonuclease Mutant Virus Replication Is Revealed by Complete Genome SequencingResearch Article Published on 2010-05-062022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] accumulate acute respiratory syndrome Author bioinformatics clone compared competent Complete complex consensus genome coronavirus coronavirus exoribonuclease Coronaviruses Critical Culture decrease deep sequencing demonstrated Effect Evolution examined experiment Frequency full-length genome Genetic Genome genome replication genome sequence growth Impact in vitro inactivation increase in Infidelity lack less limit mechanism MOST murine hepatitis virus mutant mutant virus Mutation nonstructural protein nonstructural protein 14 nsp14 nsp14-ExoN Nsps passage Pathogenesis powerful tool quasispecy recognize Replication replication fidelity required resulting Revealed RNA genome RNA genomes RNA virus SARS-coronavirus SARS-CoV sequence stability the Coronaviridae to define total population unique viral growth viral replication viral RNA genome viral system virus wild-type SARS-CoV [DOI] 10.1371/journal.ppat.1000896 PMC 바로가기 [Article Type] Research Article
In Vitro Reconstitution of SARS-Coronavirus mRNA Cap MethylationResearch Article Published on 2010-04-222022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 2003 Activation activity acute respiratory syndrome addition Author CAP cellular mRNA Complete complex coronavirus Coronaviruses CoV Critical drug design enzyme etiological agent event expression generate Genome host cell in viral in vitro inactive include induce infected cell Influenza virus inhibition assay initiated involved membrane-bound methylated Methylation methyltransferase mRNA mRNAs Mutation non-structural protein nsp14 predicted produced Protein replicate ribosomes RNA RNA genome RNA synthesis RNA transcript S-adenosyl-L-methionine SARS-coronavirus SARS-CoV SARS-CoV infected cells selective sequence target translation variety viral mRNAs viral nonstructural proteins Viral protein virus [DOI] 10.1371/journal.ppat.1000863 PMC 바로가기 [Article Type] Research Article
Detection of novel respiratory viruses from influenza‐like illness in the Philippines †Research Article Published on 2010-04-192022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] collected coronavirus country detect detected detection Epidemiology HKU1 human bocavirus human metapneumovirus Inc KI virus Melaka virus métapneumovirus nasopharyngeal aspirate NL63 not found novel virus Patient PCR performed polymerase chain Respiratory samples respiratory virus respiratory viruses RT‐PCR specimens subtype Transcription tropical virus WU virus [DOI] 10.1002/jmv.21763 PMC 바로가기 [Article Type] Research Article
Structural and functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virusArticle Published on 2010-04-142022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] activities Ala Amino acid amino acid substitution Analysis Atlantic salmon bovine Bovine coronavirus catalytic site Combination determinant different disease domain enzymatic activity enzyme expression feature functional had no Hemagglutinin-esterase Hemagglutinin-esterase (HE) infectious Infectious salmon anaemia virus (ISAV) Influenza introduced molecular orthomyxovirus Protein protein structure Proteins purification Quantitative Receptor binding Receptor-destroying enzyme (RDE) Recombinant secretory HE (recHE) red blood cells representing resolved responsible selected Ser Serine Structural analysis Surface glycoprotein variants virulence virulent virus [DOI] 10.1016/j.virusres.2010.03.020 PMC 바로가기 [Article Type] Article
SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent MechanismResearch Article Published on 2010-04-082022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] acute lung injury acute respiratory syndrome affecting alveolar inflammation ARDS Author BALB/c mice caused cell proliferation clinical disease coronavirus death demonstrated Dependent died Diffuse alveolar damage ENhance Epidemic evaluated highly pathogenic I, II independent Infected Infection innate immune innate immune response Innate immunity interferon interferon signaling knockout mice lack liver Lung disease Lung injury Lung pathology MA15 mechanism mice molecular mechanism Mortality Mouse models other tissue outcomes Pathogenesis Pathways Post-infection PROTECT Protein pulmonary fibrosis pulmonary lesion question receptor regulated respiratory respiratory virus SARS Coronavirus SARS pathogenesis SARS-CoV SARS-CoV infection severe severe disease Signaling spleen Stat1 the epidemic Type type I type III interferon viral pathogenesis virus virus pathogenesis virus titer Virus-host interaction viruses weight loss wildtype [DOI] 10.1371/journal.ppat.1000849 PMC 바로가기 [Article Type] Research Article