Toll-like receptors, chemokine receptors and death receptor ligands responses in SARS coronavirus infected human monocyte derived dendritic cellsResearch Article Published on 2009-06-082022-10-27 Journal: BMC Immunology [Category] Coronavirus, [키워드] 2003 Apoptosis biological significance Blood CCR chemokine Chemokine receptor compared DC DCs death dendritic cell explain expression Fa facilitate Gene Gene Expression Immune escape induce Infected Infection involved Ligand lung infiltration lymph nodes lymphocyte lymphoid mechanism Migration modulate monocyte outbreak provide receptor reported response Result SARS Coronavirus SARS patients SARS-CoV severity significantly TLRs Toll-like receptor Toll-like receptors TRAIL unique upregulation virus [DOI] 10.1186/1471-2172-10-35 PMC 바로가기 [Article Type] Research Article
The SARS-Unique Domain (SUD) of SARS Coronavirus Contains Two Macrodomains That Bind G-QuadruplexesResearch Article Published on 2009-05-152022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome ADP-ribose amino-acid Apoptosis Author bats bind binding coding compared component concerning coronavirus crystal structure determine determined different domain Evidence extreme form found function functional G-quadruplex gel electrophoresis Genome genomic RNA help homodimer host cell humans immune response in vitro include Infected involved lysine macrodomain mild infection monomer mRNA Mutation non-structural protein nucleic acid nucleotides nucleus occur oligonucleotide other coronaviruses outbreak pathogenicity Possible Protein protein 3 reduction Region regulate related virus remained Replication residue residues responsible RNA SARS Coronavirus SARS-CoV selected Seven shown Signal transduction Structure structures target three-dimensional structure Transcription viral infection viral replication virus X-ray crystallography [DOI] 10.1371/journal.ppat.1000428 PMC 바로가기 [Article Type] Research Article
Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine developmentArticle Published on 2009-05-042022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] approach avian coronavirus Avian coronavirus infectious bronchitis virus backbone Cell culture coronavirus demonstrated Dengue different Effect Embryo Express expression firefly luciferase gene fluorescent found Gene gene delivery Gene Expression Genome Host IBV infectious Infectious Bronchitis Virus inoculated intrinsic manipulation ORF6 Protein reverse genetics stability tissue Vaccine Vaccine development vector viral gene viral infection Viral vector virus viruses [DOI] 10.1016/j.jviromet.2009.04.023 PMC 바로가기 [Article Type] Article
Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryosResearch Published on 2009-02-052022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] addition Affect age Allantoic fluid Antigen Brain bronchitis Cell chorioallantoic collected detected Diagnosis diagnostic different disease Embryo embryonic embryos fixed found gastrointestinal tract generate IBV IBV infection IHC immunohistochemistry induce Infected Infection Infectious Bronchitis Virus inoculated kidney kidneys liver lung M41 membrane MOST Myocardial nasal epithelium nervous system Neuron oviduct Pathogenesis PI Respiratory system Result sensitivity spleen strain Strains tissue tissue tropism vasculature virulence virus virus isolation [DOI] 10.1186/1743-422X-6-15 PMC 바로가기 [Article Type] Research
A computational analysis of SARS cysteine proteinase-octapeptide substrate interaction: implication for structure and active site binding mechanismResearch Published on 2009-01-302022-10-27 Journal: BMC Bioinformatics [Category] Coronavirus, [키워드] approach binding binding mechanism carried complex computational analysis contribute cysteine drug design electrostatic enzyme Hypothesis involved MD simulation MD simulations molecular molecular docking proteinase Replication Research residue respiratory Result SARS Coronavirus severe shown turn understanding virus α-helix [DOI] 10.1186/1471-2105-10-S1-S48 PMC 바로가기 [Article Type] Research
Molecular characterization of a canine respiratory coronavirus strain detected in Italy ☆Article Published on 2009-01-202022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Accessory genes bovine Bovine coronavirus canine canine respiratory coronavirus CoVs described detected different disease ENCODE envelope protein feature Gene Genetic Genomic 3′-end genomic region Genotype host tropism humans Italian Italy mutant non-structural protein Point mutation Protein Recombination related Respiratory Coronavirus responsible RNA virus sequence Sequence analysis structural protein suggested tissue unique Viral RNA virus [DOI] 10.1016/j.virusres.2008.12.011 PMC 바로가기 [Article Type] Article
MyD88 Is Required for Protection from Lethal Infection with a Mouse-Adapted SARS-CoVResearch Article Published on 2008-12-122022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] 2003 absence adapter addition animal model antiviral therapies Atypical Author BALB/c mice C57BL/6 caused Cell characterized chemokine Chemokine receptor chest X-ray China circulating Clinical course compared contribute coronavirus correlated Course CoV death disease disease pathogenesis early response exhibited expression Factor function Genetic greater Host host gene Host immune response humans identify immune immune system Infection Inflammatory Inflammatory response innate immune intranasal Laboratory lethal Lower respiratory tract lung Lung inflammation lung tissue Lungs mice monocyte monocytes/macrophage Mortality mouse-adapted SARS-CoV Pneumonia Post-infection Proinflammatory cytokine protection Protein provide Pulmonary pathology receptor recruitment Replication required respiratory virus respond SARS-CoV SARS-CoV infection severe lung pathology Signaling significantly higher Spread susceptible TLR Travel Viral load viral loads viral replication virus wild-type [DOI] 10.1371/journal.ppat.1000240 PMC 바로가기 [Article Type] Research Article
Human airway epithelial cell culture to identify new respiratory viruses: Coronavirus NL63 as a modelArticle Published on 2008-12-052022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] airway amplification amplify antibody approach approaches Cell culture cell line clinical samples coronavirus CPE, cytopathic effect Culture culture supernatant cultures Cytopathic effect described detect determine different time point epithelial evaluated experiment HAE HAE culture HAE, human airway epithelium HCoV-NL63 HCoV-NL63, human coronavirus NL63 Human identify Infection lack molecular NL63 nucleic acid pathogen Pathogens Propagation reagent reported respiratory pathogen respiratory viral respiratory virus SARS-CoV, severe acute respiratory syndrome coronavirus secretion sequence Sequence analysis TEM TEM, transmission electron microscopy Transcriptome transmission electron microscopy variety virus Whole transcriptome amplification WTA, whole transcriptome amplification [DOI] 10.1016/j.jviromet.2008.10.022 PMC 바로가기 [Article Type] Article
Plaque assay for human coronavirus NL63 using human colon carcinoma cellsMethodology Published on 2008-11-122022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] African green monkey Antiviral Avicel carboxymethyl-cellulose Cell Cell culture colds Colon coronavirus cytopathogenic effect days post infection demonstrated different disease Drug screening Effects HCoV HCoV-NL63 Human human pathogen humans Infection kidney log NL63 plaque Plaque assay plaque assays produced purification quantification replicated Result Support susceptibility tested utility viral infectivity virus virus infectivity virus replication virus stock [DOI] 10.1186/1743-422X-5-138 PMC 바로가기 [Article Type] Methodology
Broadening of Neutralization Activity to Directly Block a Dominant Antibody-Driven SARS-Coronavirus Evolution PathwayResearch Article Published on 2008-11-072022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 80R activity amino acid change Analysis Anti-viral Anti-viral therapy Author block caused CDR Combination complementarity determining region component Cytidine deaminase dominant driving effective elicited Epidemic epitope escape mutant escape mutants escape mutation Evidence Evolution found generate human convalescent sera immune immune pressure IMPROVE Infected Infectious disease information introduced investigated Mutagenesis NAb natural infection neutralization neutralization activity neutralizing antibody non-immune outbreak pathway Pathways Positive selection Protein provided response responses against responsible SARS Coronavirus SARS-CoV SARS-CoV spike protein Somatic Spike protein strain Strategy Support Therapies transfer Transmission viral variant virus viruses while zoonotic zoonotic transmission [DOI] 10.1371/journal.ppat.1000197 PMC 바로가기 [Article Type] Research Article