Development of potent and effective synthetic SARS-CoV-2 neutralizing nanobodies강력하고 효과적인 합성 SARS-CoV-2 중화 나노바디 개발Article Published on 2021-01-012022-09-12 Journal: mAbs [Category] 진단, [키워드] acute respiratory syndrome affected angiotensin-converting enzyme 2 antibody binding candidate cellular receptor characterized coronavirus coronavirus disease COVID-19 COVID-19 pandemic described development domain effective evaluated humanized in vivo nanobody neutralize Neutralizing neutralizing antibody Outbreaks phage display Prevent prophylactically respiratory virus responsible SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 spike the SARS-CoV-2 therapeutic therapeutic intervention toolkit viral infection virus wildtype [DOI] 10.1080/19420862.2021.1958663 PMC 바로가기 [Article Type] Article
High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme고친화성 나노바디는 SARS-CoV-2 스파이크 수용체 결합 도메인과 인간 안지오텐신 전환 효소의 상호작용을 차단합니다Article Published on 2020-12-222022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 변종, 진단, [키워드] ACE2 angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 antibody fragment approved Biochemistry block COVID-19 pandemic derivative diagnostic Drug development effective enzyme expressing form HEK293 cell HEK293 cells high affinity human ACE2 Infection inhalation Interaction lead lentivirus Medical research nanobodies nanobody nebulization potency Proteins pseudotyped Receptor binding domain responsible SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein receptor binding domain Spike protein the SARS-CoV-2 the spike protein therapeutic Treatment variant virus [DOI] 10.1038/s41598-020-79036-0 PMC 바로가기 [Article Type] Article
An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive SpikeReport Published on 2020-12-182022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome affinity airway epithelia angiotensin-converting enzyme 2 Anti-spike antibody Bacteria bind binding cells coronavirus cryo-EM disrupt group host cell host cell receptor inaccessible inactive Interaction intravenously mammalian cell maturation Microscopy multivalent nanobodies nanobody neutralization neutralize potent produced Protein Receptor binding domain SARS-CoV-2 SARS-COV-2 infection Science screened selected spike stability synthetic nanobody the spike protein therapeutic Treatment trivalent virus yeast [DOI] 10.1126/science.abe3255 PMC 바로가기 [Article Type] Report
Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2Report Published on 2020-12-182022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome affinity Anti-spike antibody Bacteria cells complex Concentration coronavirus COVID-19 pandemic group half-maximal inhibitory concentration identify immunization inactive indicated inhibit intravenously mammalian cell mechanisms microbe multivalent nanobodies nanobody neutralization neutralization potency neutralize Neutralizing nonoverlapping epitope potent Prevent produced proteomics RBD Receptor binding domain SARS-CoV-2 Science screened selected Spike protein stability synthetic nanobody the RBD the spike protein therapeutic viral infection yeast [DOI] 10.1126/science.abe4747 PMC 바로가기 [Article Type] Report
Picomolar SARS-CoV-2 Neutralization Using Multi-Arm PEG Nanobody Constructs다중 팔 PEG 나노바디 구조를 사용한 피코몰 SARS-CoV-2 중화Article Published on 2020-12-112022-09-01 Journal: Biomolecules [Category] MERS, SARS, 변종, 진단, [키워드] alkyne approach azide C-terminus click chemistry component components creating demonstrated functional functional groups fusion glycol group Infectious virus ligation multivalent Multivalent antibody nanobodies nanobody neutralization neutralization assays neutralize neutralized Neutralizing PEG linker Polyethylene glycol promoted SARS-CoV-2 SARS-CoV-2 pseudotyped virus single-domain antibody fragment small molecule sortase A terminal the SARS-CoV-2 variant viability virus [DOI] 10.3390/biom10121661 PMC 바로가기 [Article Type] Article
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2Article Published on 2020-11-042022-10-29 Journal: Nature Communications [Category] COVID-19, SARS, [키워드] ACE2 binding ACE2 binding site Analysis antibody production approach bind Biochemistry Biophysics coronavirus SARS-CoV-2 costly COVID-19 COVID-19 pandemic cryo-EM cryo-EM structure high affinity Infectious diseases Isolation Molecular medicine nanobody nanomolar affinity neutralization activity neutralize neutralized Neutralizing activity neutralizing antibody Pseudotyped viruses pseudovirus RBD SARS-CoV-2 SARS-CoV-2 spike protein Selection spike Spike protein Structural analysis structural biology sybody synthetic nanobody the receptor-binding domain the SARS-CoV-2 viruses [DOI] 10.1038/s41467-020-19204-y PMC 바로가기 [Article Type] Article
Development of humanized tri-specific nanobodies with potent neutralization for SARS-CoV-2Article Published on 2020-10-202022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, SARS, [키워드] Analysis antibodies antibody Antibody therapy approach binding Combination coronavirus COVID-19 COVID-19 pandemic described develop development domain epitope Features fused generate Health human ACE2 receptor human IgG1 Fc humanized identify impacted Interaction lack monoclonal naïve nanobody neutralization protein stability resulting SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 spike protein severity therapeutic therapy Treatment unmet need [DOI] 10.1038/s41598-020-74761-y PMC 바로가기 [Article Type] Article
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interactionArticle Published on 2020-09-042022-10-04 Journal: Nature Communications [Category] SARS, 진단, [키워드] ACE2 antibodies antibody Bacteria bind binding complex conformation conformations COVID-19 Cryo-electron microscopy Cryoelectron microscopy domain enzyme epitope expressed high affinity host cell identify immunize Interaction Intervention Isolation nanobody neutralize presenting pseudovirus RBD RBD-ACE2 receptor Receptor binding domain reveal SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 spike SARS-CoV-2 spike protein spike glycoprotein target the RBD the SARS-CoV-2 while [DOI] 10.1038/s41467-020-18174-5 PMC 바로가기 [Article Type] Article
Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 Research Published on 2020-07-132022-10-05 Journal: Nature Structural & Molecular Biology [Category] SARS, 유전자 메커니즘, 진단, [키워드] ACE2 ACE2 binding antibody bind binding domain cause Cell complex coronavirus CR3022 cryo-EM crystal structure epitope fusion H11-H4 human Angiotensin-converting enzyme Influenza Interaction maturation nanobodies nanobody neutralization Neutralizing activity overlap produced RBD receptor recognize SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV-2 Spike RBD SARS-CoV-2 virus spike trimer the spike protein viral entry [DOI] 10.1038/s41594-020-0469-6 [Article Type] Research
An update on antiviral antibody-based biopharmaceuticalsArticle Published on 2020-07-062022-11-01 Journal: International immunopharmacology [Category] Coronavirus, SARS, [키워드] antibody antibody fragment Antibody fragments Antiviral biopharmaceuticals COVID-19 diagnostic Diseases Epidemics help high affinity immune system mAb microorganisms molecular monoclonal antibody nanobody neutralize recognizing scFv target targets therapeutic therapeutic strategy treatment approach variety viral infections Viral targets virus [DOI] 10.1016/j.intimp.2020.106760 PMC 바로가기 [Article Type] Article