Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospitalComparative Study Published on 2020-08-012022-10-06 Journal: Clinical microbiology and infection : the official [Category] SARS, 진단, [키워드] 95% CI Accuracy acute respiratory syndrome Analysis C-reactive protein (CRP Characteristics Cohort coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CRP diagnostic diagnostics disease eligible ELISA evaluated heterogeneous hospital immunochromatographic assay individual Lateral lateral flow lateral flow immunoassay molecular NAT NATs nucleic acid amplification Orient Patient patient control group patients plasma sample presenting Rapid rapid serological test Rapid test respiratory sample respiratory symptom SARS-CoV-2 sensitivity sera serology severe acute respiratory syndrome coronavirus 2 specificity Symptom Symptoms Test Variability [DOI] 10.1016/j.cmi.2020.05.028 PMC 바로가기 [Article Type] Comparative Study
A Rapid, Simple, Inexpensive, and Mobile Colorimetric Assay COVID-19-LAMP for Mass On-Site Screening of COVID-19Article Published on 2020-07-292022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, SARS, [키워드] activities COVID-19 COVID-19 pandemic detect Detection limit Diagnosis diagnostic equipment evaluated eyes highest indicated isothermal amplification mass Mass screening Mobile mobile Diagnostic Nasopharyngeal swab on-site screening other respiratory virus patients with COVID-19 point-of-care positive Prevent qPCR qRT-PCR Rapid reduce respiratory sample Respiratory virus infection RT-LAMP Saliva Sample SARS-CoV-2 SARS-CoV-2 RNA Screening sensitivity simple specificity throat swab Transmission Viral load [DOI] 10.3390/ijms21155380 PMC 바로가기 [Article Type] Article
Variplex™ test system fails to reliably detect SARS-CoV-2 directly from respiratory samples without RNA extractionOriginal Article Published on 2020-07-172022-10-31 Journal: European Journal of Clinical Microbiology & Infect [Category] COVID-19, MERS, SARS, [키워드] age COVID COVID diagnosis COVID-19 diagnostic testing demonstrated detect Endpoint false-negative rate False-negative reactions gold standard hospital infectious disease outbreaks isolated RNA isothermal amplification Loop-mediated isothermal amplification Patient PCR PCR assay PCR-confirmed performed positive Prevalence reported respiratory respiratory sample Respiratory samples RNA RNA extraction SARS-CoV-2 screened Sensitivity and specificity Specificity and sensitivity tested [DOI] 10.1007/s10096-020-03983-9 PMC 바로가기 [Article Type] Original Article
Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisationRapid Communication Published on 2020-07-052022-10-28 Journal: Virus Evolution [Category] COVID-19, [키워드] Amplicon sequencing amplicon-based enrichment approach ARTIC Network bioinformatics capture method characterisation Complete consensus sequence Coverage COVID-19 COVID-19 outbreak DNA enrichment evaluated Genome genome coverage genomes Genomic surveillance Genomics identity Illumina metagenomic metagenomic sequencing next generation sequencing NGS ONT ORF6 Oxford Nanopore Technology Panel performed producing protocol reference sequence Region required respiratory respiratory sample SARS-CoV-2 sequenced Sequencing SNP threshold Viral load WGS whole-genome sequencing [DOI] 10.1093/ve/veaa075 PMC 바로가기 [Article Type] Rapid Communication
SARS-CoV-2 does not replicate in embryonated hen’s eggs or in MDCK cell linesRapid Communication Published on 2020-06-252022-10-31 Journal: Eurosurveillance [Category] COVID-19, SARS, [키워드] cell line COVID-19 Influenza influenza vaccines Influenza virus Influenza, SARS-CoV-2, vaccine, respiratory, air-borne infections, viral infections, influenza investigated platform potential risk replicate respiratory sample risk SARS-CoV-2 SARS-CoV-2 replication severe acute respiratory syndrome – SARS, influenza virus, biosafety, vaccines and immunisation, laboratory substrate Support vaccine production [DOI] 10.2807/1560-7917.ES.2020.25.25.2001122 PMC 바로가기 [Article Type] Rapid Communication
Rapid Direct Nucleic Acid Amplification Test without RNA Extraction for SARS-CoV-2 Using a Portable PCR ThermocyclerArticle Published on 2020-06-182022-10-31 Journal: Genes [Category] COVID-19, SARS, [키워드] acid acute respiratory syndrome amplification caused coronavirus coronavirus disease country COVID-19 Diagnosis diagnostics Direct direct-PCR extraction homogeneous Laboratory molecular nasal Nucleic acid purification nucleocapsid pandemic PCR point-of-care polymerase chain portable PCR protocol Rapid reagents reduce Region Research respiratory sample reverse transcription RNA RNA copy RNA purification RT-PCR SARS-CoV-2 sputum Test thermocycler [DOI] 10.3390/genes11060664 PMC 바로가기 [Article Type] Article
Features of enteric disease from human coronaviruses: Implications for COVID‐19Review Published on 2020-06-052022-10-04 Journal: Journal of medical virology [Category] MERS, SARS, 치료기술, [키워드] Accuracy acute respiratory syndrome Affect amplification Asymptomatic individuals asymptomatic patient Care coronavirus Coronavirus infections Coronaviruses Course COVID‐19 COVID‐19 cumulative disease disease onset Endemic excrete excretion facilitated Factor gastroenteritis Gastrointestinal Genetic Health human coronavirus illness Incubation period Infection infection control initial intestinal Laboratory less Live virus nonviable virus pandemic Patient public health respiratory sample SARS‐CoV‐2 specimens Stool Symptom the patient Viral RNA virus virus detection [DOI] 10.1002/jmv.26066 PMC 바로가기 [Article Type] Review
Point-of-Care Diagnostic Tests for Detecting SARS-CoV-2 Antibodies: A Systematic Review and Meta-Analysis of Real-World DataReview Published on 2020-05-182022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] 95%CI carried characterized concerning conducted contagious infection COVID-19 COVID-19 diagnosis COVID-19 outbreak Diagnostic method feasibility global pandemic gold standard healthcare facility heterogeneity Laboratory Laboratory testing limitations management Meta-analysis Molecular tests point-of-care diagnostics polymerase chain Rapid diagnostic test rapid testing real-world data required respiratory sample responsible review risk RT-PCR SARS-CoV-2 sensitivity specificity systematic review systematic review and meta-analysis Test therapeutic intervention [DOI] 10.3390/jcm9051515 PMC 바로가기 [Article Type] Review
COVID‐19 associated pulmonary aspergillosisOriginal Article Published on 2020-05-152022-10-30 Journal: Mycoses [Category] COVID-19, MERS, [키워드] acute respiratory distress ARDS Aspergillus Complication comprehensive analysis coronavirus disease COVID‐19 Critically ill patient detect ECMO evaluate FIVE Germany ICU intensive care invasive isavuconazole moderate objective Patient patients with ARDS respiratory sample Result retrospective risk SARS‐CoV‐2 severe ARDS subject surgical syndrome university viral infection voriconazole [DOI] 10.1111/myc.13096 PMC 바로가기 [Article Type] Original Article
Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samplesArticle Published on 2020-04-222022-11-01 Journal: Journal of Clinical Virology [Category] COVID-19, SARS, [키워드] acute respiratory syndrome ad hoc approach assist caused China coronavirus COVID-19 case Effectiveness FIVE Germany Hospitalized hospitals Laboratory Minipools MONITOR Novel coronavirus nucleic acid nucleic acid extraction objective one individual pandemic Patient positive protocol public health public health measures real-time RT-PCR respiratory sample Respiratory samples Result RNA RT-PCR SARS-CoV-2 SARS-CoV-2 surveillance SARSCoV-2 Surveillance tested [DOI] 10.1016/j.jcv.2020.104381 PMC 바로가기 [Article Type] Article