SARS-CoV-2 Whole-Genome Sequencing by Ion S5 Technology-Challenges, Protocol Optimization and Success Rates for Different StrainsIon S5 기술에 의한 SARS-CoV-2 전체 게놈 시퀀싱 - 다양한 균주에 대한 과제, 프로토콜 최적화 및 성공률Article Published on 2022-06-062022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] Amplicon arise assigned cDNA Concentration construction COVID-19 COVID-19 pandemic demonstrated diagnostics Effectiveness Emergency genomic data idea immunization Ion less library library preparation lineages Modification molecular Molecular epidemiology mRNA mRNA or viral vector vaccines Mutation Next-generation sequencing NGS optimization Panel Patient Poland produced protocol Public public health emergency Rate Real-time PCR required RT-PCR SARS-CoV-2 SARS-CoV-2 evolution SARS-CoV-2 lineage SARS-CoV-2 sequencing Scientific community Sequencing strain Support Surveillance Thermo Fisher Scientific variant variants variants of concern Viral Viral load Viral RNA Viral vector vaccines whole-genome whole-genome sequencing [DOI] 10.3390/v14061230 PMC 바로가기 [Article Type] Article
A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assayHRM 및 프로브 기반 RT-PCR 분석을 사용하여 쿠르드-이란 인구의 다섯 번째 피크에서 SARS-COV-2의 우세한 변이를 식별하기 위한 스크리닝 전략Article Published on 2022-06-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, [키워드] caused country D614G D614G mutation Del69-70 mutation diagnostic test dominant variant E484K E484K mutation E484Q mutation genomic Genomic surveillance high resolution HRM identify indicated Iran Kurdistan L452R L452R mutation melting mortality rate Mutation operation overlapped positive patient probe Probe-based RT-PCR reduce Resolution RT-PCR RT-PCR assay SARS CoV SARS CoV-2 SARS-CoV-2 positive patients screened screening strategy Sequencing suggested Surveillance T478K T478K mutation. Transmissibility variant variants whole-genome sequencing [DOI] 10.1016/j.jviromet.2022.114514 PMC 바로가기 [Article Type] Article
First description of a multisystemic and lethal SARS-CoV-2 variant of concern P.1 (Gamma) infection in a FeLV-positive catFeLV 양성 고양이의 다중전신 및 치명적인 SARS-CoV-2 변종 우려 P.1(감마) 감염에 대한 첫 번째 설명Case Reports Published on 2022-05-262022-09-11 Journal: Virology Journal [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome analyses Analysis analyzed animal bat bats carried cats causative agent clinical evaluation Co-infection contribute COVID-19 death Gamma Host host adaptation Immunocompromised Infection Laboratory lesion lesions maintenance male Multi-systemic viral infection multisystemic Mutation organs P.1 Phylogenetic studies phylogenetic study presenting question raise reservoir respiratory RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant Severe acute respiratory syndrome Spread susceptible Trachea Transmission variants Viral viral infection virus VoC whole-genome sequencing [DOI] 10.1186/s12985-022-01816-z PMC 바로가기 [Article Type] Case Reports
Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification관심 식별의 SARS-CoV-2 변이체에 대한 신속하고 접근 가능한 역전사-정량적 PCR 접근 방식의 평가Article Published on 2022-05-182022-09-11 Journal: Journal of Clinical Microbiology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alpha amplification approach assist Beta Clinical prognosis clinical settings collected complementary concern coronavirus COVID-19 Delta demonstrated E484K epidemiological epidemiological surveillance Gamma Genetic identification infections K417N L452R Laboratory Local multiplex Mutation N501Y nucleic acid nucleic acid amplification nucleic acid amplification tests omicron PCR quantitative PCR Rapid reaction response RT-qPCR SARS-CoV-2 SARS-CoV-2 nucleic acid Sequencing severe acute respiratory syndrome Coronavirus Spike protein Surveillance swab sample T478K therapy Transmissibility upper respiratory tract vaccination validation variant variants of concern VOCs WGS whole-genome sequencing [DOI] 10.1128/jcm.00178-22 PMC 바로가기 [Article Type] Article
Molecular Analysis of SARS-CoV-2 Lineages in Armenia아르메니아에서 SARS-CoV-2 계통의 분자 분석Article Published on 2022-05-172022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (Alpha) affected Alpha Analysis Armenia caused circulation clade coronavirus COVID-19 Delta Epidemiology epitope Epitopes functional Genome genomic HLA Illumina Illumina sequencing information Lineage loci molecular Molecular epidemiology Mutation N gene N genes Nanopore nanopore sequencing omicron ORF1 ORF1ab PCR test PCR tests performed positive Protective provide Research RT-PCR SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sequence sequences Sequencing short-read sequencing signal specific clade specific clades Surveillance Transmission utility variants Viral viral evolution virus whole-genome sequencing [DOI] 10.3390/v14051074 PMC 바로가기 [Article Type] Article
Rapid and Accurate Identification of SARS-CoV-2 Variants Using Real Time PCR AssaysReal Time PCR 어세이를 사용한 SARS-CoV-2 변이체의 빠르고 정확한 식별Article Published on 2022-05-102022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] Coronavirus, COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] allele Alpha B.1.1.7 B.1.351 B.1.617.2 Beta blinded Combination correlated COVID-19 COVID-19 pandemic Delta delta variant Diagnosis diagnostic testing disease severity E484K effort Epidemiology evade Gamma Genomic surveillance genotyping help host immunity identification Laboratory lack Lineage management Mutation N501Y omicron P.1 P681R Patient Patient management PCR positive primer Rapid real-time RT-PCR resource reverse transcription-PCR routine diagnostic testing RT-PCR S982A SAR-CoV-2 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants screened Sequencing specimen specimens spike gene Surveillance susceptibility tested the SARS-CoV-2 Therapeutics These data time Transmissibility variant variants Viral variants WGS while whole-genome sequencing [DOI] 10.3389/fcimb.2022.894613 PMC 바로가기 [Article Type] Article
Nosocomial Outbreak by Delta Variant From a Fully Vaccinated Patient완전 예방 접종을 받은 환자의 Delta 변이에 의한 병원 발병Article Published on 2022-05-022022-09-11 Journal: Journal of Korean medical science [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Admission Airborne transmission airflow Care Caregiver CCTV ChAdOx1 ChAdOx1 nCoV-19 close contact Contact contact with coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Delta delta variant diagnosed with COVID-19 Diagnosis dominant driven by equipment FIVE had no healthcare worker hospital index case individual Inpatient nosocomial occurred outbreak Patient performed polymorphism positive Sample SARS-CoV-2 severe acute respiratory syndrome Coronavirus single-nucleotide single-nucleotide polymorphism Symptom tested vaccinated individual vaccination variant virus ward WGS whole-genome sequencing [DOI] 10.3346/jkms.2022.37.e133 PMC 바로가기 [Article Type] Article
Risk factors, immune response and whole-genome sequencing of SARS-CoV-2 in a cruise ship outbreak in Norway노르웨이 유람선 발병 시 SARS-CoV-2의 위험 요인, 면역 반응 및 전체 게놈 시퀀싱Article Published on 2022-05-012022-09-11 Journal: International Journal of Infectious Diseases [Category] COVID19(2023년), SARS, 치료기술, [키워드] 95% confidence interval adjusted odds ratio Analysis antibody approach Asia attack rate Blood Blood samples Chain Reaction Cluster community transmission conducted COVID-19 cross-sectional cross-sectional study Cruise ship Epidemiology Factors Final high risk immune response Immunity IMPROVE Infection infection control investigated Isolation Lineage Local male measure median age multidisciplinary approach nasopharynx national health authorities nationality Norway outbreak Outbreak investigation PCR PCR-negative polymerase chain polymerase chain reaction quarantine reported SARS-CoV-2 SARS-CoV-2 polymerase chain reaction Sequencing seropositive Transmission whole-genome sequencing [DOI] 10.1016/j.ijid.2022.02.025 PMC 바로가기 [Article Type] Article
A SARS-CoV-2 Delta variant containing mutation in the probe binding region used for RT-qPCR test in Japan exhibited atypical PCR amplification and might induce false negative result일본에서 RT-qPCR 테스트에 사용된 프로브 결합 영역에 돌연변이를 포함하는 SARS-CoV-2 Delta 변이체가 비정형 PCR 증폭을 나타내어 위음성 결과를 유발할 수 있음Article Published on 2022-05-012022-09-11 Journal: Journal of infection and chemotherapy : official j [Category] COVID19(2023년), SARS, 변종, [키워드] activities amplification curve amplification curves Analysis analyzed Atypical binding caused COVID-19 database de novo delta variant Delta variants diagnose diagnosis of SARS-CoV-2 Diagnostic quantitative RT-qPCR Endemic exhibited followed by in some induce infected with SARS-CoV-2 Infection Japan MONITOR mutant Mutation N gene nucleotide pandemic PCR amplification performed Phylogenetic analysis Point Point mutation probe qPCR Quantitative reported robust RT-qPCR RT-qPCR test SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-positive specimen severe health problem severe health problems single nucleotide Spread variant variants variants of SARS-CoV-2 virus whole-genome sequencing [DOI] 10.1016/j.jiac.2022.01.019 PMC 바로가기 [Article Type] Article
Limited cross-species transmission and absence of mutations associated with SARS-CoV-2 adaptation in cats: A case study of infection in a small household setting고양이의 SARS-CoV-2 적응과 관련된 제한된 종간 전파 및 돌연변이 부재: 소규모 가정 환경에서의 감염 사례 연구Article Published on 2022-05-012022-09-11 Journal: Transboundary and emerging diseases [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] absence adaptation adaptive Anosmia Asymptomatic cat cats chain Clinical signs close contact collected Concentration concentrations Course COVID-19 Cross-species transmission Diagnosis Epidemiology fatigue flat highlight Human human SARS-CoV-2 strain human SARS-CoV-2 strains immunosuppressive Infection infections initial Interaction investigated Limited loss of taste Mutation naïve natural infection retroviral RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 strains SARS-CoV-2 transmission Seroconversion Serological testing sneezing Support Swab symptom onset Taste titre Transmission Viral RNA virus virus titre virus transmission were measured whole-genome sequencing [DOI] 10.1111/tbed.14132 PMC 바로가기 [Article Type] Article