Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCRSARS-CoV-2 RT-PCR에 대한 풀링된 비인두 또는 타액 표본에 대한 자동화된 고처리량 액체 기반 RNA 추출 플랫폼 평가Article Published on 2021-04-022022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] automated comparable COVID-19 COVID-19 pandemic Ct values detect diagnostics Efficiency extraction GAPDH inhibitors Large-scale testing liquid-based nucleic acid extraction magnetic bead Mass screening Nasopharyngeal swab no significant differences nucleic acid nucleic acid extraction Oropharyngeal paucity of data platform pooled specimens positive positive rate real-time RT-PCR RNA RNA extraction RT-PCR Saliva saliva specimen saliva specimens SAR-CoV-2 SARS-CoV-2 SARS-CoV-2 RT-PCR significant difference significant differences specimen specimens the SARS-CoV-2 was performed was used [DOI] 10.3390/v13040615 PMC 바로가기 [Article Type] Article
Genomic surveillance of SARS-CoV-2 in the Republic of Congo콩고공화국에서 SARS-CoV-2의 게놈 감시Article Published on 2021-04-012022-09-11 Journal: International Journal of Infectious Diseases [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 African assigned carried clade Clusters collected contributing to coronavirus Ct values D614G database Genome genomes Genomic surveillance Illumina individual information Lineage Lineage B1 Mutation Next-generation sequencing NGS pangolin provide Republic of Congo RT-PCR SARS CoV SARS CoV-2 SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 lineage SARS-CoV-2 sequence SARS-CoV-2 variants screened sequenced severe acute respiratory syndrome Coronavirus shown spike mutation submitted Surveillance Transmission virus WGS Whole genome sequencing whole-genome sequencing [DOI] 10.1016/j.ijid.2021.03.036 PMC 바로가기 [Article Type] Article
Evaluation of SARS-CoV-2 in Tears of Patients with Moderate to Severe COVID-19중등도에서 중증 COVID-19 환자의 눈물에서 SARS-CoV-2 평가Article Published on 2021-04-012022-09-11 Journal: Ophthalmology [Category] SARS, 치료기술, [키워드] 48 hour 48 hours amplification curve Analysis Chain Reaction collected Comorbidities conjunctival Conjunctival swab coronavirus disease Coronavirus disease 2019 COVID-19 cross-sectional study Ct value Ct values cycle threshold E gene enrolled eye Frame gold gold standard group laboratory confirmation manifestation median value moderate moderate disease moderate to severe nasopharyngeal Nasopharyngeal swab ocular Open reading frame outcome Patient patients patients with moderate polymerase chain polymerase chain reaction positive positive result positive RT-PCR profiles real-time RT-PCR reverse-transcriptase polymerase chain reaction RNA-dependent RNA polymerase RT-PCR analysis RT-PCR assay Sample SARS-CoV-2 SARS-CoV-2 RNA severe coronavirus disease severe COVID-19 severe disease significantly higher specimen statistically significant Swab Symptomatology systemic symptoms Tear tears threshold transported Viral load Viral transmission viral transport media was determined [DOI] 10.1016/j.ophtha.2020.08.029 PMC 바로가기 [Article Type] Article
A double-edged sword: Prolonged detection of SARS-COV-2 in patients receiving cancer directed therapy양날의 검: 암 지시 치료를 받는 환자에서 SARS-COV-2의 장기 탐지Article Published on 2021-04-012022-09-11 Journal: Seminars in oncology [Category] SARS, 치료기술, 치료제, [키워드] analyzed black Breast Cancer center Columbia University conducted Consent COVID-19 Ct values demographics described detectable detection Disease progression Gastrointestinal greater help Hispanic histology identify IgG antibodies IgG antibody Infection interruption malignancy median time Mild mild to moderate Mild to moderate COVID-19 Mild-to-moderate Moderate COVID-19 morbidity and mortality nasopharyngeal nine Patient patients patients with asymptomatic Patients with cancer PCR PCR assay PCRs POD positive Prolonged receiving recommendations repeated required Retrospective study risk Sarcoma SARS-CoV-2 serology serology testing Seven swabs target tested therapy threshold time Tumor Viral load were recorded white worsening [DOI] 10.1053/j.seminoncol.2020.11.001 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms안구 증상이 없는 환자의 결막 분비물에서 SARS-CoV-2 검출Article Published on 2021-04-012022-09-11 Journal: Infection [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Appropriate Asymptomatic Chain Reaction collected Conjunctiva conjunctival Conjunctival secretion Conjunctival secretions Conjunctival swab conjunctival swabs coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients Ct values Culture detection duration of illness evaluate infection control laboratory-confirmed measure median nosocomial Nosocomial transmission ocular ocular symptoms Patient polymerase chain reaction positive Prevent ranged reverse transcription Reverse transcription-polymerase chain reaction RT-PCR SARS-CoV-2 SARS-CoV-2 RNA secretion severe acute respiratory syndrome Coronavirus shedding Swab Symptom Symptoms transmission of SARS-CoV-2 Undetermined viral culture was obtained [DOI] 10.1007/s15010-020-01524-2 PMC 바로가기 [Article Type] Article
Comparative evaluation of four SARS-CoV-2 antigen tests in hospitalized patientsComparative Study Published on 2021-04-012023-06-19 Journal: International Journal of Infectious Diseases [Category] MERS, SARS, [키워드] antigen test COVID-19 Ct values Infectivity RT-PCR SARS-CoV-2 [DOI] 10.1016/j.ijid.2021.02.052 PMC 바로가기 [Article Type] Comparative Study
Mass molecular testing for COVID19 using NGS-based technology and a highly scalable workflowNGS 기반 기술과 고도로 확장 가능한 워크플로를 사용하여 COVID19에 대한 질량 분자 테스트Article Published on 2021-03-292022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, [키워드] Accuracy assays Brazil chain Chain Reaction Chinese contribute Coronavirus infection country COVID19 Ct value Ct values cycle database demonstrated dependent on develop Diagnosis diagnostic equipment feature Genome Genomics Government help Illumina Infection Infectious-disease diagnostics involved mass Microbial genetics molecular Molecular diagnosis multiplex New coronavirus Next-generation sequencing Pandemics pathogen performance platform provided reaction reported researcher respiratory reverse transcription rising RNA extraction RNA sequencing RT-PCR RT-qPCR Sample SARS-CoV-2 Sequencing significantly syndrome Usage Virology whole genome Wuhan Wuhan, China [DOI] 10.1038/s41598-021-86498-3 PMC 바로가기 [Article Type] Article
Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosisSARS-CoV-2 진단을 위한 현장 진단 테스트로서의 비색 역전사 루프 매개 등온 증폭의 필수 속성 및 함정Research Article Published on 2021-03-262022-09-10 Journal: Molecular Medicine [Category] SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] addition clinical samples Cluster color change colorimetric detection correlation Ct values demonstrated described detect determinate Diagnosis diagnosis of COVID-19 diagnostic diagnostics Essential false-negative false-negative results indication indications Internal control Interpretation lead Loop-mediated isothermal amplification manuscript molecular Molecular biology molecular diagnostics nuance performed point of care point-of-care positive produced quantification reducing Result resulting reverse transcription RT-LAMP RT-qPCR SARS-CoV-2 sensitivity specificity supplementary material Symptom technique Viral Viral load virus with color [DOI] 10.1186/s10020-021-00289-0 PMC 바로가기 [Article Type] Research Article
A faster and less costly alternative for RNA extraction of SARS-CoV-2 using proteinase k treatment followed by thermal shock열 충격 후 proteinase k 처리를 사용하여 SARS-CoV-2의 RNA 추출을 위한 더 빠르고 저렴한 대안Research Article Published on 2021-03-242022-09-10 Journal: PLoS ONE [Category] SARS, 진단, 치료기술, 치료제, [키워드] addition alternative method average conducted costly COVID-19 Critical Ct values decrease detect diagnose Effectiveness epidemiological experiment faster followed by house initial INS Internal control lack less measure molecular Molecular diagnosis Molecular tests not differ pandemic Patient proteinase Proteinase K protocol reagents real time RNA RNA extraction RT-PCR RT-PCR reaction RT-PCR reactions SARS-CoV-2 SARS-COV-2 infection Shock significantly submitted targets tested the disease the SARS-CoV-2 Treatment Viral virus spread [DOI] 10.1371/journal.pone.0248885 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 is associated with high viral loads in asymptomatic and recently symptomatic healthcare workersSARS-CoV-2는 무증상 및 최근 증상이 있는 의료 종사자의 높은 바이러스 부하와 관련이 있습니다.Research Article Published on 2021-03-182022-09-12 Journal: PLoS ONE [Category] MERS, SARS, 진단, [키워드] accounted addition analyzed Asymptomatic Asymptomatic COVID-19 Community coronavirus disease Coronavirus disease 2019 COVID-19 Ct value Ct values cycle threshold duration of symptom effort equipment exposure to HCW healthcare worker hospital increased risk Inpatient Inpatients maintain median Nasopharyngeal swab occurred Outpatient outpatients Patient positive Result risk RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 test short duration significantly Symptom symptom duration symptomatic Symptoms translated Viral load with COVID-19 [DOI] 10.1371/journal.pone.0248347 PMC 바로가기 [Article Type] Research Article