Development of multi-specific humanized llama antibodies blocking SARS-CoV-2/ACE2 interaction with high affinity and avidityLetter Published on 2020-05-222022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, SARS, [키워드] ACE2 receptor antibody approach bi-specific antibody binding Combination combination therapies contribute COVID-19 development diagnostics domains facilitate high affinity host cell host cell membranes humanized humanized antibody in-silico Interaction limitation llama antibody MERS monoclonal MOST multiple mechanism nanobody overcome pandemic polyclonal Protein S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus significantly synergistic therapeutic tri-specific antibody trimeric spike unique viral disease virus entry [DOI] 10.1080/22221751.2020.1768806 PMC 바로가기 [Article Type] Letter
Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid AntibodiesArticle Published on 2020-05-052023-06-15 Journal: Cell [Category] MERS, SARS, [키워드] COVID-19 MERS nanobody SARS SARS-CoV-2 [DOI] 10.1016/j.cell.2020.04.031 PMC 바로가기 [Article Type] Article
A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virusResearch Published on 2019-09-162022-10-29 Journal: Journal of Nanobiotechnology [Category] Coronavirus, [키워드] antibody biotinylated Biotinylated-nanobody Blocking ELISA characterized cutoff value detect Diagnosis Diagnostic method diarrhea Efficacy ELISA epidemic diarrhea epidemiological evaluate the effect expression fusion protein high mortality rate immunization in vitro Infection N protein nanobody overcome PEDV periplasmic problem purified required Result Sensitivity and specificity serological serum serum sample stability strain tested time-consuming Vaccine virus vomiting watery [DOI] 10.1186/s12951-019-0531-x PMC 바로가기 [Article Type] Research
Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassayResearch Published on 2019-03-012023-08-30 Journal: Journal of Nanobiotechnology [Category] E형 간염, [키워드] antibody competitive ELISA nanobody Nanobody-HRP NDV [DOI] 10.1186/s12951-019-0468-0 PMC 바로가기 [Article Type] Research
A Novel Nanobody Targeting Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Receptor-Binding Domain Has Potent Cross-Neutralizing Activity and Protective Efficacy against MERS-CoVVaccines and Antiviral Agents Published on 2018-06-272022-10-30 Journal: Journal of Virology [Category] Coronavirus, MERS, [키워드] activity antibody Antiviral antiviral therapeutics binding binding site C-terminal clearance conformational conserved Critical cross-neutralization cross-neutralize cross-neutralizing domain Efficacy epitope Epitopes exhibited expressed expression feasibility high affinity Human humanized in vivo infect Infection mapped MERS-CoV MERS-CoV infection MERS-CoV RBD mice Middle East nanobody Neutralizing novel potent potential limitation produced producing Prophylactic PROTECT protective efficacy RBD receptor Receptor-binding domain recognizing renal residue respiratory respiratory syndrome coronavirus significantly Spike protein Spread strain targeting tested the RBD the receptor-binding domain therapeutic agents therapeutic efficacy Treatment yeast [DOI] 10.1128/JVI.00837-18 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Monitoring liver macrophages using nanobodies targeting Vsig4: concanavalin A induced acute hepatitis as paradigmArticle Published on 2015-02-012023-05-31 Journal: Immunobiology [Category] A형 간염, [키워드] Complement receptor of the Ig superfamily (CRIg) Concanavalin A (ConA) Hepatitis Kupffer cells nanobody SPECT/μCT V-set and immunoglobulin domain-containing 4 (Vsig4). [DOI] 10.1016/j.imbio.2014.09.018