Novel coronavirus-like particles targeting cells lining the respiratory tractResearch Article Published on 2018-09-052022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] ACE2 protein baculovirus Cell ciliated cell concerning displaying evaluated expressed expressing expression HCoV-NL63 Major novel Particle platform produced Production purification purified Research respiratory tract structural protein tissue tropism vaccine candidates Viral vector VLP VLPs [DOI] 10.1371/journal.pone.0203489 PMC 바로가기 [Article Type] Research Article
Nucleocapsid protein-dependent assembly of the RNA packaging signal of Middle East respiratory syndrome coronavirusResearch Published on 2018-05-242022-10-29 Journal: Journal of Biomedical Science [Category] Coronavirus, [키워드] addition assembly C-terminal domain coexpressed Culture dependent on expression functional GFP help Heterologous HUH7 cells indicated membrane MERS MERS-CoV MERS-CoV genome Microscopy Middle East mRNA N-terminal domain nucleocapsid protein nucleotide Particle positive-sense Protein respiratory syndrome coronavirus Result RNA RNA fragment RNA genome RNA packaging signal RNA structure SARS-CoV sequence single-stranded structural protein structural proteins the viral nucleocapsid viral genome virus virus infection virus particle VLP VLPs was determined [DOI] 10.1186/s12929-018-0449-x PMC 바로가기 [Article Type] Research
The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette StrainResearch Article Published on 2017-01-122022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] attachment biochemically Cell cellular entry cellular membrane centrifugation cholesterol coronavirus demonstrated depleting early stage expression Factor genome replication host cell IBV IBV infection IBV-infected cell indicated Infectious Bronchitis Virus Infectivity Lipid marker Mevastatin non-structural proteins plasma membrane reduced required role significantly stages strain structural protein structural proteins suppressed Treatment Vero Cell virion viruses [DOI] 10.1371/journal.pone.0170123 PMC 바로가기 [Article Type] Research Article
Molecular Characterization and Phylogenetic Analysis of Porcine Epidemic Diarrhea Viruses Associated with Outbreaks of Severe Diarrhea in Piglets in Jiangxi, China 2013Research Article Published on 2015-03-192022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] accumulated Amino acid amino acid substitution Analysis analyzed antigenic caused change Characteristics characterization characterized China Chinese Complete contagious death diarrhea Effectiveness epidemic diarrhea epitope Genetic Genetic variation Genome genome sequence hydrophilicity identify identity less Multiple Mutation N gene Neutralizing nucleotide ORF1a/b ORF3 outbreak PEDV proximal ranged S gene strain Strains structural protein unique Vaccine viral disease virus viruses vomiting watery [DOI] 10.1371/journal.pone.0120310 PMC 바로가기 [Article Type] Research Article
Manipulation of the Porcine Epidemic Diarrhea Virus Genome Using Targeted RNA RecombinationResearch Article Published on 2013-08-022022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] addition Asian cause China deleted demonstrated diarrhea ENCODE epidemic diarrhea expression expression cassette functional Gene Genes genetics GFP Heterologous in vitro manipulation Modification ORF3 PEDV Protein Renilla luciferase Replication Research RNA RNA recombination structural protein the disease this system Vaccines viral genome virus virus Virus neutralization [DOI] 10.1371/journal.pone.0069997 PMC 바로가기 [Article Type] Research Article
Severe acute respiratory syndrome coronavirus accessory proteins 6 and 9b interact in vivoArticle Published on 2012-07-202022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] accessory protein accessory proteins acute respiratory syndrome Amino acid Analysis analyzed approach cellular conditions coronavirus Coronaviruses cytoplasm described eight ENCODE experiment Factor functions Genome homology immunoprecipitation in vivo infective Interaction involved LC–MS pathogenicity Protein Protein 6 Protein 9b protein–protein interactions Proteins proteomic Replication SARS Coronavirus SARS-CoV SARS-CoV infected cell SARS-CoV infected cells severe shown structural protein Viral protein virus [DOI] 10.1016/j.virusres.2012.07.012 PMC 바로가기 [Article Type] Article
Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane proteinResearch Published on 2010-01-152022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] accessory protein addition Alpha Amino acid Analysis antiserum C-terminus Cell conserved coronavirus coronavirus Coronaviruses cytosolic detected digestion ENCODE ERGIC Evidence expression Extracellular FLAG-tagged fluorescence microscopy Frame function Golgi Huh-7 cell Human Human coronavirus immunofluorescence In-vitro Infected lysosomal membrane membrane protein N-terminus NL63 nucleocapsid Occurrence ORF pathogenic coronavirus predicted Protein purified reading Respiratory tract infections Result sequence shown structural protein subsequent Topology translation viral particle virion [DOI] 10.1186/1743-422X-7-6 PMC 바로가기 [Article Type] Research
Molecular characterization of a canine respiratory coronavirus strain detected in Italy ☆Article Published on 2009-01-202022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Accessory genes bovine Bovine coronavirus canine canine respiratory coronavirus CoVs described detected different disease ENCODE envelope protein feature Gene Genetic Genomic 3′-end genomic region Genotype host tropism humans Italian Italy mutant non-structural protein Point mutation Protein Recombination related Respiratory Coronavirus responsible RNA virus sequence Sequence analysis structural protein suggested tissue unique Viral RNA virus [DOI] 10.1016/j.virusres.2008.12.011 PMC 바로가기 [Article Type] Article
Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main ProteasesResearch Article Published on 2005-10-062022-10-28 Journal: PLoS Biology [Category] Coronavirus, [키워드] anti-CoV drug antigens Antiviral antiviral activity assays cellular cellular entry Cluster complexes Compound conserved coronavirus CoV CoVs crystal structure disease Diseases drug enzyme enzyme activity gastroenteritis Gene Expression Genetic homology model host receptor Human in viral in vitro inactivation Infection inhibitor M pro mechanism Modification Mutation NO pathogen prevalent Prevent protease recombination rate Replication representing structural protein Structure supported targeting Toxicity Vaccines virus [DOI] 10.1371/journal.pbio.0030324 PMC 바로가기 [Article Type] Research Article
G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cellsShort Report Published on 2005-08-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] amino acids Analysis Annexin Apoptosis apoptosis induction Atypical Cell cell cycle arrest cells apoptosis characterized coronavirus COS-7 cell COS-7 cells ENCODE expression found fusion protein Genome immune induce infect labeling life-threatening liver lung mechanism Necrosis non-structural protein non-structural proteins organ organs over-expression Pathogenesis Pneumonia predicted Protein Proteins related Replication respiratory SARS pathogenesis SARS-CoV severe similarity structural protein Till transfected cell Vero virulence viruses [DOI] 10.1186/1743-422X-2-66 PMC 바로가기 [Article Type] Short Report