Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunityResearch Article Published on 2020-09-172022-10-30 Journal: JCI Insight [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Adaptive immunity antibody Antibody detection Antibody Response binding antibody circulating antibody collected coronavirus correlation COVID-19 COVID-19 immunity detection ELISA exhibited Generation Health healthy IgG antibody immune response Immunity immunology implication individual moderate N-protein neutralize Neutralizing antibodies neutralizing capacity nucleocapsid pandemic patients positive Protein Quantitative Receptor binding domain reverse transcription S-RBD SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 spike sera Seroconversion serological serology assay serology testing serum sample Spike protein the SARS-CoV-2 Viral protein virus infection [DOI] 10.1172/jci.insight.142386 PMC 바로가기 [Article Type] Research Article
Blocking of the High-Affinity Interaction-Synapse Between SARS-CoV-2 Spike and Human ACE2 Proteins Likely Requires Multiple High-Affinity Antibodies: An Immune PerspectiveImmunology Published on 2020-09-172022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] ACE2 ACE2 protein ACE2 proteins adhere animal model antibodies antibody binding-affinity block blocking caused classical Combination comparison COVID-19 Critical crystal structure develop effective eight epithelial epithelial cells epitope felines Host Human human ACE2 IgA dimer Infection Interaction interaction-synapse interactions interface interfere intestine Multiple Neutralizing antibodies neutralizing antibody pandemic Protein protein-protein interaction Proteins SARS-CoV SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 transmission significant difference single antibody spike therapeutic therapy treating COVID-19 Vaccine virus X-ray [DOI] 10.3389/fimmu.2020.570018 PMC 바로가기 [Article Type] Immunology
Competitive SARS-CoV-2 Serology Reveals Most Antibodies Targeting the Spike Receptor-Binding Domain Compete for ACE2 BindingResearch Article Published on 2020-09-162022-10-30 Journal: mSphere [Category] COVID-19, SARS, [키워드] ACE2 ACE2 binding ACE2-Fc acute respiratory syndrome angiotensin-converting enzyme 2 anti-RBD Anti-RBD antibodies Anti-RBD antibody antibodies antibody Antigen approach assessment Cohort coronavirus coronavirus disease COVID-19 determine disease domain ELISA enzyme-linked immunosorbent assay epitope generate highlight human Angiotensin-converting enzyme humoral immune response immunoserology Infection Interaction interactions majority MOST Neutralizing antibodies Patient patient sera promote proportion Protein RBD Receptor-binding domain required responsible SARS-CoV-2 SARS-CoV-2 patient sera Serological assay serology serum samples spike Spread targeting the SARS-CoV-2 the SARS-CoV-2 virus the Spike utility Vaccine variant viral entry virus [DOI] 10.1128/mSphere.00802-20 PMC 바로가기 [Article Type] Research Article
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution SerologyArticle Published on 2020-09-162023-06-18 Journal: Cell [Category] Fulltext, SARS, [키워드] Coronaviruses COVID-19 effector functions Immunity Neutralizing antibodies SARS-CoV-2 [DOI] 10.1016/j.cell.2020.09.037 PMC 바로가기 [Article Type] Article
Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID-19 Patients혈청학적 분석, 회복된 COVID-19 환자에서 매우 가변적인 SARS-CoV-2 중화 항체 활성 추정Immunoassays Published on 2020-09-112022-09-10 Journal: Journal of Clinical Microbiology [Category] SARS, 진단, 치료기술, [키워드] 2019 Abbott Abbott SARS-CoV-2 IgG Accuracy activity acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 anti-SARS-CoV-2 antibody antiviral activity center choice collected convalescent plasma convalescent plasma samples coronavirus coronavirus disease correlated COVID-19 Critical determine Donor ELISA ELISAs enrolled enzyme enzyme-linked immunosorbent assay enzyme-linked immunosorbent assays estimate gold gold standard house IgG Immunity immunoglobin Infection Interpretation levels of antibodies levels of antibody methodology NAb NAb activity NAb assay NAb assays NAbs neutralization Neutralizing antibodies neutralizing antibody New York offer pandemic Particles patient sera Population population immunity predict Program proportion Pseudotyped virus recovered reopening respiratory SARS-CoV-2 SARS-CoV-2 detection Sensitivity and specificity serologic tests Serological assay serological assays serological measurement serological measurements serological test Serological tests serology Seroprevalence severe acute respiratory syndrome Coronavirus therapeutic therapeutic trials total Ig Trial unique vaccination variable Variation Viral viral infection [DOI] 10.1128/JCM.02005-20 PMC 바로가기 [Article Type] Immunoassays
Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 BindingResource Published on 2020-09-032022-10-31 Journal: Cell [Category] COVID-19, [키워드] ACE2 ACE2 binding ACE2 receptor Affect Amino acid Analysis Antibody therapeutics COVID-19 dataset deep deep mutational scanning Deleterious determine dominant Effect ENhance Evidence expression facilitate folded protein functional annotation host range identify interface residue isolate isolates MOST Mutation Neutralizing antibodies pandemic RBD RBD expression Receptor binding domain Receptor-binding domain Region SARS-CoV-2 SARS-CoV-2 pandemic SARS-related coronaviruses scanning selected spike glycoprotein Spike protein Substitution Surveillance target the RBD the SARS-CoV-2 Vaccine Vaccine design virus [DOI] 10.1016/j.cell.2020.08.012 PMC 바로가기 [Article Type] Resource
The Rapid Coronavirus Antibody Test: Can We Improve Accuracy?Medicine Published on 2020-09-022022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] Can Lateral flow assays Neutralizing antibodies paper based analytical devices Rapid Rapid tests SARS-CoV-2 antibodies [DOI] 10.3389/fmed.2020.00569 PMC 바로가기 [Article Type] Medicine
Naturally occurring SARS-CoV-2 gene deletions close to the spike S1/S2 cleavage site in the viral quasispecies of COVID19 patientsResearch Article Published on 2020-09-022022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] accumulate ACE2 receptor binding Burn circulation Complete COVID19 patient Deletion deletions described Diversity facilitate frameshift generate Genome host cell Infection insertions Mild Mutation rates natural selection Naturally Neutralizing antibodies NGS other coronaviruses Patient Protein Quasispecies Recombination reducing released respiratory virus S1 protein S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 S gene SARS-CoV-2 spike severe COVID-19 severe COVID-19 patients severity stop codon tissue damage Transmission Treatment upstream Vaccine development viral particle viral quasispecy virus [DOI] 10.1080/22221751.2020.1806735 PMC 바로가기 [Article Type] Research Article
Evaluate severe acute respiratory syndrome coronavirus 2 infectivity by pseudoviral particles유사바이러스 입자에 의한 중증급성호흡기증후군 코로나바이러스 2 감염성 평가Article Published on 2020-09-012022-09-11 Journal: Journal of medical virology [Category] SARS, 진단, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 biosafety biosafety level BSL-2 laboratory Characteristics coronavirus evaluate Human identify indicated infect Infection infective Infectivity Laboratory level 2 neutralization Neutralizing antibodies neutralizing antibody nucleocapsid outbreak Particle Particles potency pseudoviral particles. recovering patient respiratory rodents SARS-CoV-2 serum severe acute respiratory syndrome Coronavirus Spike protein Spread the spike protein tissue tropism Vaccines was used [DOI] 10.1002/jmv.25865 PMC 바로가기 [Article Type] Article
Airway Delivery of Anti-influenza Monoclonal Antibodies Results in Enhanced Antiviral Activities and Enables Broad-Coverage Combination TherapiesArticle Published on 2020-08-262023-06-08 Journal: Journal of Virology [Category] Fulltext, 신종인플루엔자, [키워드] antibody combination therapy Influenza Neutralizing antibodies [DOI] 10.1128/JVI.00052-20 PMC 바로가기