Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea VirusMethodology Article Published on 2015-08-012022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] addition Analysis antibody Asia asked assays biotinylated calculated colostrum Coronaviruses correlation country cross-reactivity demonstrated described develop development diagnostic test Disadvantages ELISA epidemic diarrhea expressed fluorescent Fluorescent Focus Neutralization (FFN) Fluorescent microsphere immunoassay (FMIA) functional gastroenteritis highlight IFA Immune status immunoassay immunohistochemistry Interpretation kappa value Laboratory mAb mAbs magnetic bead measurement microsphere MONITOR monoclonal monoclonal antibody multiple antigen naïve Neutralization assay Neutralizing antibodies neutralizing antibody Neutralizing antibody response North North America outbreak pathogen PEDV porcine epidemic diarrhea virus (PEDV) protective immunity quantitation question receiver operating characteristic recent Respiratory Coronavirus Result ROC ROC analysis Sensitivity and specificity Seroconversion Serological assay serology serum serum sample significant level Spread staining strain TGEV vaccine candidate virus virus neutralizing antibodies was performed were used [DOI] 10.1186/s12917-015-0500-z PMC 바로가기 [Article Type] Methodology Article
A site of varicella-zoster virus vulnerability identified by structural studies of neutralizing antibodies bound to the glycoprotein complex gHgLArticle Published on 2015-05-122023-06-02 Journal: Proceedings of the National Academy of Sciences of [Category] 두창, 홍역, [키워드] crystal structures electron microscopy gHgL Neutralizing antibodies VZV [DOI] 10.1073/pnas.1501176112 PMC 바로가기
Serological evidence of pandemic (H1N1) 2009 virus in pigs, West and Central AfricaArticle Published on 2015-03-232023-06-24 Journal: Veterinary microbiology [Category] 신종인플루엔자, [키워드] Africa influenza A Neutralizing antibodies pandemic virus swine [DOI] 10.1016/j.vetmic.2014.12.022
Replication-competent influenza A viruses expressing a red fluorescent proteinArticle Published on 2014-12-142023-06-23 Journal: Virology [Category] 신종인플루엔자, [키워드] in vivo imaging system (IVIS) 2A antivirals influenza A virus interferon mCherry Microneutralization assay Neutralizing antibodies NS1 replication-competent virus virus neutralization assay [DOI] 10.1016/j.virol.2014.12.006 PMC 바로가기 [Article Type] Article
Tailoring Subunit Vaccine Immunity with Adjuvant Combinations and Delivery Routes Using the Middle East Respiratory Coronavirus (MERS-CoV) Receptor-Binding Domain as an AntigenResearch Article Published on 2014-11-182022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] adjuvant antibody Antigen BALB/c mice Cell-mediated immunity cellular immune responses cellular immunity Combination CpG Critical cytokine production delivery effective elicited ELISpot assay evaluated Freund’s adjuvant humoral Humoral immunity hydroxide hypothesised IFA IFN-γ IgG immune response Immunity immunized immunogenic IMPROVE indicated intramuscularly investigated MERS-CoV MERS-CoV RBD mice Middle East Modification murine Neutralizing antibodies neutralizing antibody offered pandemic Protein purified RBD regimen respiratory respiratory syndrome coronavirus robust route rRBD spot-forming cell subunit T-cell Response the receptor-binding domain vaccination Vaccine was tested [DOI] 10.1371/journal.pone.0112602 PMC 바로가기 [Article Type] Research Article
A glycoprotein subunit vaccine elicits a strong Rift Valley fever virus neutralizing antibody response in sheepArticle Published on 2014-10-012023-06-02 Journal: Vector Borne and Zoonotic Diseases [Category] 조류인플루엔자, [키워드] Glycoproteins Neutralizing antibodies Rift Valley fever virus sheep Subunit vaccine [DOI] 10.1089/vbz.2014.1650 PMC 바로가기
Neutralizing antibodies against influenza A in pigs before and after the 2009 pandemic, LuxembourgArticle Published on 2014-02-212023-06-25 Journal: Veterinary microbiology [Category] 신종인플루엔자, [키워드] cross-reaction influenza A Neutralizing antibodies pandemic 2009 pig. [DOI] 10.1016/j.vetmic.2013.12.009
Lack of MERS Coronavirus Neutralizing Antibodies in Humans, Eastern Province, Saudi ArabiaDispatch Published on 2013-12-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, MERS, [키워드] coronavirus detect Human lentiviral MERS MERS coronavirus MERS-CoV MERS-CoV infection Middle East Middle East respiratory syndrome Coronavirus Neutralizing activity Neutralizing antibodies neutralizing antibody plasma pseudotyping respiratory infections respiratory syndrome coronavirus Saudi Arabia Seroprevalence serum spike tested viral spike protein viruses [DOI] 10.3201/eid1912.130701 PMC 바로가기 [Article Type] Dispatch
Serological evidence for a hepatitis e virus-related agent in goats in the United StatesArticle Published on 2013-12-012023-06-11 Journal: Transboundary and emerging diseases [Category] E형 간염, Fulltext, [키워드] animal reservoir Experimental infection goat hepatitis E virus Neutralizing antibodies Prospective Study [DOI] 10.1111/tbed.12001 PMC 바로가기
Role of the Spike Glycoprotein of Human Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Virus Entry and Syncytia FormationResearch Article Published on 2013-10-032022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] 293T cell activate Affect antibody bats Betacoronavirus blocked cathepsin L cathepsin L-dependent Cell cell type Cl cleave cleaved co-expressed coronavirus coronavirus spike glycoprotein determined elicit endosome Eptesicus experiment Express Extracellular fluids Formation function glycoprotein host cell host protease host range Human immunoblot inhibitor lentivirus maturation membrane fusion MERS MERS-CoV MERS-CoV S protein MHV Middle East murine Neutral Neutralizing antibodies New World non-infectious plasma membrane polyclonal produced producing pseudotype pseudovirion pseudovirus entry reagent receptor released Research respiratory role S protein S2 domain serine protease Spike protein Syncytia syncytia formation the S protein the Spike tissue Treatment triggered trypsin variety VERO E6 cells viral spike virus virus entry [DOI] 10.1371/journal.pone.0076469 PMC 바로가기 [Article Type] Research Article