Construction of SARS-CoV-2 Virus-Like Particles by Mammalian Expression SystemBioengineering and Biotechnology Published on 2020-07-302022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] construction COVID-19 disease expression Genetic glycosylation patterns HEK-293T cells lack mammalian membrane protein molecular Morphological morphological feature non-infectious Pandemics Particle Particles pathogenic viruses powerful tool Protein Release Research Safe SARS-CoV-2 SARS-CoV-2 VLP SARS-CoV-2 VLPs self-assemble shown small envelope structural proteins System vaccine candidate Vero E6 cell virion Virological virus virus-like particles VLP [DOI] 10.3389/fbioe.2020.00862 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Analysis of ACE2 Gene-Encoded Proteins Across Mammalian SpeciesVeterinary Science Published on 2020-07-032022-11-01 Journal: Frontiers in Veterinary Science [Category] COVID-19, [키워드] ACE2 ACE2 gene acute respiratory syndrome Analysis analyzed angiotensin-converting enzyme 2 binding caused chronic disease close contact coded coronavirus coronavirus disease correlation COVID-19 Data analysis Felis homologous homology host cell Human human ACE2 receptor humans immunosuppressive Infection infection process mammalian mammals novel Novel coronavirus Pneumonia predicted Predictive Protein public health Rattus receptor required SARS-CoV-2 Specy spike-protein suggested susceptibility susceptible Transmission virus [DOI] 10.3389/fvets.2020.00457 PMC 바로가기 [Article Type] Veterinary Science
Structural Basis for Designing Multiepitope Vaccines Against COVID-19 Infection: In Silico Vaccine Design and ValidationOriginal Paper Published on 2020-06-192022-10-29 Journal: Jmir Bioinformatics and Biotechnology [Category] COVID-19, [키워드] acute respiratory syndrome Against allele analyzed Antigen antigen processing B cell epitope B-cell Basis caused cDNA cellular complementary complex composed coronavirus COVID-19 CTL cytotoxic T lymphocyte DNA effective elicit ENhance epitope Epitopes expression host cell HTL HTL epitope human cell human transporter associated with antigen processing (TAP) humoral immune responses immune response immunoinformatics in silico In silico methods in vivo interferon leukocyte linear mammalian MEV MEVs molecular molecular docking, molecular dynamics simulation molecular interaction Multi-epitope vaccine multiepitope vaccine nonstructural protein novel coronavirus disease objective Onchocerca volvulus ORF overexpression pandemic positive-sense single-stranded RNA predicted protein sequence proteome reading Result SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccine screened secreted severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) T lymphocyte the SARS-CoV-2 Toll-like receptor Toll-like receptor (TLR) Vaccine vaccine candidate vaccine candidates Vaccine design validation was used [DOI] 10.2196/19371 PMC 바로가기 [Article Type] Original Paper
The Biology of Lactoferrin, an Iron-Binding Protein That Can Help Defend Against Viruses and BacteriaImmunology Published on 2020-05-282022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] ACE2 activity acute respiratory syndrome Against angiotensin-converting enzyme 2 Antiviral bind Bioavailability Biology Can cells cerebrospinal fluid coronavirus Coronaviruses COVID-19 pandemic Evidence host cells host receptor HSPGs immunological iron lactoferrin mammalian membrane receptors Prevent Proteoglycan receptor SARS-CoV-2 serum sulfate therapeutic transferred variety [DOI] 10.3389/fimmu.2020.01221 PMC 바로가기 [Article Type] Immunology
Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding SiteArticle Published on 2020-05-142022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] ACE2 ACE2 receptor acute respiratory syndrome addition Angiotensin converting enzyme-2 assist binding binding site Cavity Cell chitosan conserved coronavirus Coronavirus disease 2019 (COVID-19) CoV domain domains Drug discovery drug target examined exhibit facilitate feasibility feature fluctuation homotrimer host cell receptor identify indicated inhibit inhibitor inhibitors Interaction mammalian MD simulation mechanism molecular molecular docking molecular dynamics monomer novel pathway Protein RBD Receptor binding domain Region S2 subunit SARS-CoV-2 sequence variability Site spike spike glycoprotein Spike protein suggested the SARS-CoV-2 the SARS-CoV-2 virus the spike protein trimer trimer cavity Variability viral genome Virtual screening viruses [DOI] 10.3390/jcm9051473 PMC 바로가기 [Article Type] Article
Eco-Virological Preliminary Study of Potentially Emerging Pathogens in Hedgehogs ( Erinaceus europaeus ) Recovered at a Wildlife Treatment and Rehabilitation Center in Northern ItalyCommunication Published on 2020-03-012022-10-28 Journal: Animals : an Open Access Journal from MDPI [Category] COVID-19, [키워드] Erinaceus coronavirus (EriCoV) 2019-nCoV Betacoronavirus Betacoronavirus infection canine carrier center clinical disease collected Contact coronavirus Coronaviruses CoVs disease East ecological Erinaceus Europe European European hedgehog examined Fecal sample hedgehog hedgehogs high mutation rate Host humans individual Infection Influenza influenza A Interaction Italy Lineage mammalian management MOST nocturnal notable novel observé occurred pathogen Pathogens PCR-positive public health recovered rehabilitation reservoir reservoirs RNA viruses Sequencing simple tested the Coronaviridae virus virus genome virus isolation viruses wild animals wildlife [DOI] 10.3390/ani10030407 PMC 바로가기 [Article Type] Communication
Identification of a novel base J binding protein complex involved in RNA polymerase II transcription termination in trypanosomes트리파노솜에서 RNA 중합효소 II 전사 종결에 관여하는 새로운 염기 J 결합 단백질 복합체의 확인Research Article Published on 2020-02-212022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Ablation addition African sleeping sickness Analysis base binding binding domain cause co-IP complex dephosphorylation derepression described DNA downstream genes Epigenetic eukaryotes expand expressed expression Factors gene cluster gene clusters Gene Expression Genome homolog Host Human humans identification identify immune evasion Interaction involved lead leads Leishmania mammalian mass spectrometry mechanism Modification mutant mutants organism Organisms organized phosphatase promoter Protein protein complex Protozoa Reciprocal recruitment regulate regulated Regulatory residue RNA RNA Polymerase II RNA-Seq RNAi RT-PCR shown significantly silent sleeping sickness specific proteins sucrose surface Surface glycoprotein termination thymine Transcription transcription start sites Trypanosoma Trypanosoma brucei Trypanosome upstream variant Variant Surface Glycoprotein Wdr82 yeast [DOI] 10.1371/journal.pgen.1008390 PMC 바로가기 [Article Type] Research Article
Transcriptional and genomic parallels between the monoxenous parasite Herpetomonas muscarum and Leishmania일산화 기생충 Herpetomonas muscarum과 Leishmania 사이의 전사 및 게놈 유사점Research Article Published on 2019-11-112022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] biting flies causative agents conserved Culture Diptera disease Drosophila melanogaster early stage early stages event Gene regulation Genome genomic highlighting Host hosts Human identify in vitro infect Infection infections insect Insects Interaction Leishmania Leishmania major Leishmaniasis less mammal mammalian mammals monoxenous parasites MOST Neglected NTDs parasite parasites Regulation reported Sand similarity sleeping sickness Transcriptome Transmission transmitted Tropical Diseases Trypanosome turn vector [DOI] 10.1371/journal.pgen.1008452 PMC 바로가기 [Article Type] Research Article
The CspC pseudoprotease regulates germination of Clostridioides difficile spores in response to multiple environmental signalsCspC pseudoprotease는 여러 환경 신호에 대한 응답으로 Clostridioides difficile 포자의 발아를 조절합니다Research Article Published on 2019-07-052022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] addition Alter Amino acid Bacteria bile acid bile acids calcium Clostridioide Clostridioides conserved Critical crystal structure detect dormant exhibited function germination Gut hypo induce Infection initiate involved lack mammalian mechanism mechanism of action Mutation nosocomial Organisms pathogen physically question raise receptor regulate residue sensitivity Signaling small molecule Small molecules spore thought transmembrane unique variant while [DOI] 10.1371/journal.pgen.1008224 PMC 바로가기 [Article Type] Research Article
A viral metagenomic survey identifies known and novel mammalian viruses in bats from Saudi ArabiaResearch Article Published on 2019-04-102022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Adenoviridae Adenovirus bat bats Caliciviridae collected Coronaviridae coronavirus Ebola Egyptian fruit bat Egyptian tomb bat Evidence Fecal sample genomic homology identify implicated individual Kingdom mammalian MERS coronaviruses metagenomic Middle East molecular one health ORF1 Papillomaviridae Paramyxoviridae Phylogenetic Polyomaviridae Raby Reoviridae reservoir Rousettus Saudi Arabia Sequencing Surveillance Taphozous virus zoonotic [DOI] 10.1371/journal.pone.0214227 PMC 바로가기 [Article Type] Research Article