Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution meltingResearch Article Published on 2021-11-222022-10-28 Journal: PLoS ONE [Category] COVID-19, SARS, [키워드] Accuracy acquisition Analysis approach bases Complete country COVID-19 COVID-19 pandemic COVID-19 testing detect diagnostic diagnostics E gene facilitated genotyping globe gold standard Internal control Laboratory melting methodology Moderate COVID-19 molecular Molecular diagnosis nasopharyngeal swabs pathogen Patient PCR PCR assay PCR products PCR testing primer protocol purine RdRP reagent reagents real time Region Resolution resulting RT-qPCR RT-qPCR assay Saliva SARS-CoV-2 SARS-CoV-2 genome sensitivity sequence serum specificity specimen specimens TaqMan targets temperature the SARS-CoV-2 genome validation viral genome [DOI] 10.1371/journal.pone.0260087 PMC 바로가기 [Article Type] Research Article
Sampling site for SARS-CoV-2 RT-PCR—An intrapatient four-site comparison from Tampere, FinlandSARS-COV-2 RT-PCR 용 샘플링 사이트-핀란드 탬퍼에서의 4 개 사이트 비교.Research Article Published on 2021-11-162022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 치료기술, [키워드] analysed anatomical Anterior can be used collected COVID-19 positive patient Cycle threshold value Cycle threshold values Diagnosis heterogeneity nasal Nasal sample nasal swab nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs nasopharynx no significant difference objective Oropharyngeal oropharynx patients positive provided recruited Result RT-PCR Saliva sampling SARS-CoV-2 SARS-CoV-2 detection significant difference situation Swab written Informed Consent [DOI] 10.1371/journal.pone.0260184 PMC 바로가기 [Article Type] Research Article
A mask-based diagnostic platform for point-of-care screening of Covid-19코로나19 현장진단을 위한 마스크 기반 진단 플랫폼Article Published on 2021-11-152022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 진단, 치료기술, [키워드] approaches Aptamers breath Chain Reaction collected COVID-19 detect detection diagnostic Electrochemistry Exhaled breath condensate (EBC) Face mask form mediator Nasopharyngeal swab nasopharyngeal swabs ORF1ab Patient performed pfu platform point-of-care polymerase chain polymerase chain reaction positive quantification Real-time reverse-transcription polymerase chain reaction (RT-PCR) RT-PCR SARS-CoV-2 SARS-CoV-2 gene SARS-CoV-2 genes SARS-COV-2 infection SARS-CoV-2 positive patients SARS-CoV-2 viral particle SARS-CoV-2 viral particles screened suspensions the patient Viral particles Volume [DOI] 10.1016/j.bios.2021.113486 PMC 바로가기 [Article Type] Article
Diagnostic Yield of Saliva for SARS-CoV-2 Molecular Testing in Children소아에서 SARS-CoV-2 분자 검사를 위한 진단적 타액 수율Article Published on 2021-11-112022-09-11 Journal: Journal of the Pediatric Infectious Diseases Socie [Category] MERS, SARS, 진단, 치료기술, [키워드] Aptima SARS-CoV-2 assay children comparable demonstrated molecular Molecular assay Nasopharyngeal swab nasopharyngeal swabs noninvasive NPs pediatric Safe Saliva SARS-CoV-2 SARS-CoV-2 detection sensitivity specificity specimen Testing Viral viral loads yield [DOI] 10.1093/jpids/piab058 PMC 바로가기 [Article Type] Article
Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens임상 표본에서 SARS-COV-2 단백질을 탐지하는 도구로서 표적화 된 프로테오믹스Research Article Published on 2021-11-112022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 유전자 메커니즘, 치료기술, 치료법, 치료제, [키워드] abundance applied COVID-19 COVID-19 outbreak COVID-19 patient Critical Ct values detect diagnostics infected Vero E6 cells intensity limit of detection mass spectrometry methodology nasopharyngeal swabs nucleocapsid protein PCR peptides Protein proteomics Research SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins sensitivity specimen specimens sputum swabs tryptic peptide tryptic peptides Vero E6 VERO E6 cells Viral Viral protein Viral proteins Viral RNA [DOI] 10.1371/journal.pone.0259165 PMC 바로가기 [Article Type] Research Article
Early Adoption of Longitudinal Surveillance for SARS-CoV-2 among Staff in Long-Term Care Facilities: Prevalence, Virologic and Sequence Analysis장기 요양 시설 직원의 SARS-CoV-2 종단 감시 조기 채택: 유병률, 바이러스 및 염기서열 분석Research Article Published on 2021-11-102022-09-12 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus adoption advanced age Analysis Asymptomatic asymptomatic infections Care Characteristics Cohort collected Comorbidities Complete contributed coronavirus COVID-19 death declining epidemiological Epidemiology event Genetic Genetic epidemiology genomes genomic Impact incidence individuals Infection infections Infectious disease Infectious virus less Long-term care longitudinal nasal swab Nasopharyngeal swab nasopharyngeal swabs notable outcome pandemic pathogen performed persistence presence of comorbidities Prevalence risk factor SARS-CoV-2 SARS-CoV-2 dynamics SARS-COV-2 infection SARS-CoV-2 pandemic SARS-CoV-2 PCR sequenced severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spread staff subset Support Surveillance surveillance testing symptomatic the SARS-CoV-2 The United States Transmission Transmission dynamics transmission of SARS-CoV-2 unique United States Viral RNA virus vRNA Workplace [DOI] 10.1128/Spectrum.01003-21 PMC 바로가기 [Article Type] Research Article
Diagnostic Performance of Self-Collected Saliva Versus Nasopharyngeal Swab for the Molecular Detection of SARS-CoV-2 in the Clinical Setting임상 환경에서 SARS-CoV-2의 분자 검출을 위한 자가 채취 타액 대 비인두 면봉의 진단 성능Research Article Published on 2021-11-032022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] CT value acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 be positive can be used Care cause children clinic clinical collected Community community settings Contact coronavirus coronavirus disease coronavirus disease 19 COVID-19 COVID-19 pandemic COVID-19 symptom COVID-19 symptoms criteria cycle threshold detect detection Diagnosis diagnosis of SARS-CoV-2 diagnostic difficulty discomfort enrolled equipment etiology feasibility groups Health Health care Health care worker health care workers indicated invasive investigated median meet nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs noninvasive NPs NPSs participant Participants Patient PCR performance positive presenting professionals qPCR quantitative PCR respiratory reverse transcription risk RT-qPCR Saliva saliva samples saliva specimen saliva specimens SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 viral load Screening sensitivity Sensitivity and specificity setting severe acute respiratory syndrome Coronavirus specimen specimens Spread symptom onset target gene Target genes the patient the WHO throat swab unit Viral was performed [DOI] 10.1128/Spectrum.00468-21 PMC 바로가기 [Article Type] Research Article
Symptomatic Severe Acute Respiratory Syndrome Coronavirus 2 Reinfection of a Healthcare Worker in a Belgian Nosocomial Outbreak Despite Primary Neutralizing Antibody Response증상이 있는 중증 급성 호흡기 증후군 코로나바이러스 2 벨기에 병원 발병에서 1차 중화 항체 반응에도 불구하고 의료 종사자의 재감염Comment Published on 2021-11-022022-09-11 Journal: Clinical Infectious Diseases: An Official Publicat [Category] MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 characterized clades Cluster Clusters Contact tracing coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 detect effective Evidence evidence of first episode healthcare healthcare worker humoral humoral immune response Humoral immunity IgG immune response Immunoglobulin immunoglobulin A Immunoglobulin G immunoglobulin M Inconclusive individual individuals Infection Most patients Nasopharyngeal swab nasopharyngeal swabs Neutralizing antibodies neutralizing antibody Neutralizing antibody response neutralizing antibody responses nosocomial occur occurred outbreak Patient persistent infection potential transmission Primary infection protective immunity quantified Reinfection remained respiratory response SARS-CoV-2 Sequencing serum serum IgG severe acute respiratory syndrome Coronavirus Swab swabs symptomatic Transmission Viral viral culture viral genome viral genomes virus replication was measured was performed whole genome Whole genome sequencing worker [DOI] 10.1093/cid/ciaa1850 PMC 바로가기 [Article Type] Comment
The reliability of saliva for the detection of SARS-CoV-2 in symptomatic and asymptomatic patients: Insights on the diagnostic performance and utility for COVID-19 screening증상 및 무증상 환자에서 SARS-CoV-2 검출을 위한 타액의 신뢰도: COVID-19 스크리닝을 위한 진단 성능 및 유용성에 대한 통찰력Article Published on 2021-11-012022-09-11 Journal: Diagnostic microbiology and infectious disease [Category] SARS, 진단, 치료기술, [키워드] afebrile Asymptomatic asymptomatic individual Asymptomatic individuals collected Comorbidities coronavirus coronavirus disease COVID-19 Ct values current cycle cycle threshold demonstrated detect diagnostic evaluate febrile Infection information insight nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs Negative predictive value NPs Patient Predictive value reliability Saliva SARS-CoV-2 SARS-CoV-2 infections SARS-CoV-2-positive patients Screening screening test sensitivity specificity specimen symptomatic symptomatic and asymptomatic Symptomatic patients threshold utility Viral transmission [DOI] 10.1016/j.diagmicrobio.2021.115450 PMC 바로가기 [Article Type] Article
Evaluation of the Elecsys SARS-CoV-2 antigen assay for the detection of SARS-CoV-2 in nasopharyngeal swabs비인두 면봉에서 SARS-CoV-2 검출을 위한 Elecsys SARS-CoV-2 항원 분석 평가Article Published on 2021-11-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 치료기술, [키워드] 95% CI Accuracy Antigen antigenic assays automated Automated antigen immunoassay collected confirmation COVID-19 cut-off cut-off value diagnosed Elecsy Elecsys evaluate gold gold standard highest Nasopharyngeal swab nasopharyngeal swabs performed positive Randomly reached relative sensitivity reported RT-PCR SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 testing SARS-CoV-2 testing strategy screening strategy sensitivity specificity tested [DOI] 10.1016/j.jcv.2021.104991 PMC 바로가기 [Article Type] Article