A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoVMethodology Published on 2013-08-262022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] acute respiratory syndrome Antisera antiviral therapeutics backbone BSL-3 can be used cause Cell coronavirus demonstrated detect Dipeptidyl peptidase-4 disease domain DPP4 effective vaccine entry inhibitors evaluate exhibited expressing full-length fused highly pathogenic HIV Human serum albumin IgG Infection inhibit inhibitor inhibitors luciferase-expressing HIV-1 MERS-CoV MERS-CoV entry MERS-CoV pseudovirus mice Middle East Neutralizing antibodies neutralizing antibody Neutralizing antibody response Novel human coronavirus produced Protein pseudovirus RBD receptor residue respiratory syndrome coronavirus Result Safe significantly Spike protein variety viral entry [DOI] 10.1186/1743-422X-10-266 PMC 바로가기 [Article Type] Methodology
Structural Bases of Coronavirus Attachment to Host Aminopeptidase N and Its Inhibition by Neutralizing AntibodiesResearch Article Published on 2012-08-022022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] acute respiratory syndrome aminopeptidase aminopeptidase N antibody antigenic Antiviral APN attachment Author base binding blocked Cancer Cancer therapy Cell cell entry cell entry receptor cell membrane colds complex coronavirus CoV CoVs crystal structure determinant determine dimeric drugs ectodomain engage enteric diseases facilitate gastroenteritis Host host cells Human immune immune response in some Infection infections Infectious disease information inhibition Interaction membrane-bound neutralization Neutralizing antibodies neutralizing antibody Prevent Protein RBDs receptor Receptor binding receptor binding motif related reported residue respiratory diseases shown specificity spike glycoprotein Strains subsequent subset TGEV the RBD the receptor-binding domain the S protein unique Vaccines virus virus particle viruses [DOI] 10.1371/journal.ppat.1002859 PMC 바로가기 [Article Type] Research Article
Swine Influenza Virus Antibodies in Humans, Western Europe, 2009Research Published on 2011-03-012023-06-08 Journal: Emerging Infectious Diseases [Category] Fulltext, 신종인플루엔자, [키워드] avian-like H1N1 swine influenza virus Europe Influenza Neutralizing antibodies Pandemic (H1N1) 2009 Research swine viruses [DOI] 10.3201/eid1703100581 PMC 바로가기 [Article Type] Research
Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patientsResearch Published on 2010-11-042022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] animal model Anti-RBD antibody Antibody Response antigenicity approach assays characterized diagnostic ELISA Follow-up found Human immunogenic included induce Neutralizing activity Neutralizing antibodies neutralizing antibody potent protective response Protein purified RBD RBD of SARS-CoV RBD-specific antibody recovered patient Result robust S protein SARS patient SARS-CoV SARS-CoV spike protein serum sample the antibody response the RBD the receptor-binding domain Vaccine were measured [DOI] 10.1186/1743-422X-7-299 PMC 바로가기 [Article Type] Research
Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory SyndromeResearch Article Published on 2006-05-182022-10-29 Journal: PLoS Medicine [Category] Coronavirus, MERS, [키워드] 2003 acute respiratory syndrome Adequate affected animal animal model cause caused changed Chest radiograph children clinical disease Clinical laboratory test clinically clone coronavirus cynomolgus macaque demonstrated develop disease effective vaccine Evidence finding group Health Infected Infection infectious intravenous intravenously Jason macaque majority Mild Model moderate mucosal route nare Neutralizing antibodies NHP NHPs parameter Pathogenesis peaked Pneumonia Postinfection produced required respiratory SARS-CoV SARS-CoV infection SARS-CoV Urbani severe significantly similarity symptomatic syndrome viral replication virus were infected wild-type [DOI] 10.1371/journal.pmed.0030149 PMC 바로가기 [Article Type] Research Article
SARS Vaccine DevelopmentSynopsis Published on 2005-07-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome can be used Clinical use conformational coronavirus develop Developing development effective inactivated inducer Infection Neutralizing antibodies Neutralizing epitone neutralizing epitopes Prevent Protein RBD Receptor-binding domain Recombinant protein S protein Safe SARS SARS-CoV SARS-CoV vaccine sequence Spike protein the RBD the receptor-binding domain the S1 subunit Vaccine Vaccines vector [DOI] 10.3201/eid1107.050219 PMC 바로가기 [Article Type] Synopsis
Molecular advances in the cell biology of SARS-CoV and current disease prevention strategiesReview Published on 2005-04-152022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Antiviral antivirals Author cDNA Cell Cell Biology clone disease elicit highlight host cell infectious interact molecular molecular virology Neutralizing antibodies receptor SARS-CoV SARS-CoV vaccine the disease the SARS epidemic Vaccines Viral antigen viral genome viral genome sequence [DOI] 10.1186/1743-422X-2-35 PMC 바로가기 [Article Type] Review
Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with PseudotypesResearch Published on 2005-03-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] 2003 229E against SARS-CoV antibody coronavirus coronavirus strain correlated cross-neutralization determine develop emerging handling Hong Kong human sera Immunity in vitro Infectious diseases long-lasting microneutralization natural infection neutralization tests Neutralizing antibodies Neutralizing antibody response Neutralizing titer NO OC43 occurred outbreak Profiling pseudotype recovered patients Research response SARS patient SARS virus SARS-CoV SARS-CoV neutralizing antibodies SARS-CoV S SARS-CoV spike protein serum sample Spike protein Vaccines was used [DOI] 10.3201/eid1103.040906 PMC 바로가기 [Article Type] Research