Mixed infections with Chlamydia and porcine epidemic diarrhea virus – a new in vitro model of chlamydial persistenceResearch Article Published on 2010-07-272022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] Alter appear Cell cells Chlamydia Chlamydiaceae Chlamydiae chronic disease Co-infection Culture detected developmental elementary body epidemic diarrhea evidenced experiment experiments heterogenous homogenous hpi Human Hypothesis immunofluorescence in vitro in vitro model Inclusion induce Infection infections investigated mixed infection NO observation PEDV persistence produced Re-infection Result TEM transmission electron microscopy Vero Cell virus [DOI] 10.1186/1471-2180-10-201 PMC 바로가기 [Article Type] Research Article
Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42Research Published on 2010-05-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Analysis Cell cellular processe complex confocal microscopy coronavirus Critical detected diploid ENhance fetal fibroblast found fusion protein GST host cell host cells host protein immunofluorescence Inflammatory responses interact Interaction involved knowledge lung molecular mechanism nucleocapsid protein pathogenicity pathway Result SARS-CoV severe Spread subunit the mean variety [DOI] 10.1186/1743-422X-7-99 PMC 바로가기 [Article Type] Research
Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane proteinResearch Published on 2010-01-152022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] accessory protein addition Alpha Amino acid Analysis antiserum C-terminus Cell conserved coronavirus coronavirus Coronaviruses cytosolic detected digestion ENCODE ERGIC Evidence expression Extracellular FLAG-tagged fluorescence microscopy Frame function Golgi Huh-7 cell Human Human coronavirus immunofluorescence In-vitro Infected lysosomal membrane membrane protein N-terminus NL63 nucleocapsid Occurrence ORF pathogenic coronavirus predicted Protein purified reading Respiratory tract infections Result sequence shown structural protein subsequent Topology translation viral particle virion [DOI] 10.1186/1743-422X-7-6 PMC 바로가기 [Article Type] Research
Specific real-time reverse transcription-polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in tissues and feces from turkeys infected with turkey coronavirusArticle Published on 2009-11-142022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] 3′ end acute enteritis amplified antibody assays average Bacteria base-pair cause collected conducted coronavirus coronavirus RNA Critical detected determine Diagnosis different DPI economic loss FAM fecal Fecal sample fluorescent highest IFA immunofluorescence indicated Infected Infection inoculated interval intestine Other PCR primer positive primer probe progression quantitation Rapid reached real-time RT-PCR reporter RNA sensitivity serial dilution Specific specificity spike gene Swab swabs TaqMan probe the disease tissue Trial trials Turkey Turkey coronavirus viral genome Viral RNA viral RNA load virus was performed [DOI] 10.1016/j.jviromet.2009.11.012 PMC 바로가기 [Article Type] Article
Studies on membrane topology, N-glycosylation and functionality of SARS-CoV membrane proteinResearch Published on 2009-06-182022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] acute respiratory syndrome Amino acid analysis Analysis biochemical C-terminal Cell culture conserved coronavirus Coronaviruses ectodomain endodomain ERGIC expressing found functional glycosylated Golgi complex immunofluorescence immunogenic Interaction M protein membrane membrane protein N-glycosylation N-glycosylation site N-terminal recruitment SARS-CoV SARS-CoV M Surface glycoprotein Topology transmembrane transmembrane domain Viral particles viral spike protein virion virus [DOI] 10.1186/1743-422X-6-79 PMC 바로가기 [Article Type] Research
SARS-related Virus Predating SARS Outbreak, Hong KongDispatch Published on 2004-02-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] Adult coronavirus detected antibody dispatch healthy Hong Kong immunofluorescence neutralization assays outbreak previous exposure proportion recruited SARS SARS-CoV SARS-related virus SARS-related virus serologic study virus [DOI] 10.3201/eid1002.030533 PMC 바로가기 [Article Type] Dispatch
Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell cultureArticle Published on 1998-12-102022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] 229E Adenovirus amplification assay be applied Cell Cell culture clinical sample common cold compared coronavirus Coronaviruses cultures detected Diagnosis diagnostic diagnostic procedures disease False positive Human human coronavirus human coronaviruses 229E human infections immunofluorescence Infected infected cell infected cells Influenza virus log MOI monoclonal antibody Neurological disorders nosocomial OC43 pathology respiratory infections respiratory syncytial virus RNA RT-PCR sensitivity Serogroup specificity specimen TCID Viral RNA [DOI] 10.1016/S0166-0934(98)00013-5 PMC 바로가기 [Article Type] Article