The Nuclear Immune Receptor RPS4 Is Required for RRS1 SLH1 -Dependent Constitutive Defense Activation in Arabidopsis thaliana핵 면역 수용체 RPS4는 Arabidopsis thaliana에서 RRS1 SLH1 -의존적 구성 방어 활성화에 필요합니다Research Article Published on 2014-10-232022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] absence activate Activation Agrobacterium allele Amino acid Analysis Arabidopsis Arabidopsis thaliana Bacterial C-terminal Cell complex conducted Defense disease DNA binding domain dominant downstream effector effectors ENCODE function Genes Genetic genetic screen growth hypotheses identify Immune receptor immune receptors immune responses Immunity inactive insertion leucine loss-of-function mutation loss-of-function mutations mammalian mechanism motif mutant mutants Mutation NLR NLRs nuclear nucleotide overlap pathogen performed plant plants Protein Pseudomona Pseudomonas R genes receptor Recognition recognize required rescued responses RRS1 secretion Signaling significantly temperature transcribed transcriptional transcriptional activation transcriptional change Trigger triggers type III wild type [DOI] 10.1371/journal.pgen.1004655 PMC 바로가기 [Article Type] Research Article
Dynamically-Driven Enhancement of the Catalytic Machinery of the SARS 3C-Like Protease by the S284-T285-I286/A Mutations on the Extra DomainResearch Article Published on 2014-07-182022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] 3CL 3CLpro Ala Alter appear catalytic catalytic dyad component conducted correlated correlation Correlation analysis Critical dimeric disrupting domain domains Effects ENhance enhancement enzyme inactivated inactive include mechanisms molecular MOST mutant Mutation opposite Perturbation protease reduction replaced reported residue residues resulting reveal significantly specific inhibitor stability Trigger Volume while wild-type [DOI] 10.1371/journal.pone.0101941 PMC 바로가기 [Article Type] Research Article
Dissecting Virus Entry: Replication-Independent Analysis of Virus Binding, Internalization, and Penetration Using Minimal Complementation of β-GalactosidaseResearch Article Published on 2014-07-152022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Analysis binding Complementation coronavirus detect dissect E. coli expression Hepatitis host cell inactive internalization knowledge lack mammalian cells MHV murine mutant parameters peptide reporter vesicular stomatitis virus viral entry viral replication virus virus infection VSV β-Galactosidase [DOI] 10.1371/journal.pone.0101762 PMC 바로가기 [Article Type] Research Article
Coronavirus Papain-like Proteases Negatively Regulate Antiviral Innate Immune Response through Disruption of STING-Mediated SignalingResearch Article Published on 2012-02-012022-10-06 Journal: PLoS ONE [Category] 진단, [키워드] Activation acute respiratory syndrome Antiviral block catalytic catalytically active Cell cells complex complexes contributing to coronavirus CoV Defense disrupting Disruption domain Endoplasmic reticulum evade expressing expression form found HCoV-NL63 Host IFN IKKε inactive Infected inhibit Innate innate immune innate immune response interferon MAVS mechanism membrane mutant NL63 nuclear translocation Papain-like protease PLP promoters Protein reduced regulate required response RIG-I SARS-CoV Signaling signaling molecule subsequent TBK1 wild type [DOI] 10.1371/journal.pone.0030802 PMC 바로가기 [Article Type] Research Article
Quercetin 7-rhamnoside reduces porcine epidemic diarrhea virus replication via independent pathway of viral induced reactive oxygen speciesResearch Published on 2011-10-042022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] addition antioxidant antiviral activity Course CPE Cytopathic effect DNA fragment DNA fragmentation Effect epidemic diarrhea flow cytometry Hypothesis inactive independent induce Infection investigated NAC pathway PEDV Production quercetin reactive oxygen specy reduce reduced reduction Result ROS Vero Cell virus replication [DOI] 10.1186/1743-422X-8-460 PMC 바로가기 [Article Type] Research
PLP2 of Mouse Hepatitis Virus A59 (MHV-A59) Targets TBK1 to Negatively Regulate Cellular Type I Interferon Signaling PathwayResearch Article Published on 2011-02-182022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Activation acute respiratory syndrome assays catalytic domain cells cellular complex conserved coronavirus cytoplasm deubiquitinase domain dose-dependent manner DUB ENCODE Evidence experiment finding identification IFN IFN signaling IFN signaling pathway IFN transcription IFN-β in vitro inactivated inactive Infection inhibit inhibited innate immune system IRF3 MHV-A59 mouse mouse hepatitis virus nonstructural protein 3 Nsp3 nuclear translocation overexpression Papain-like protease phosphorylate PLP2 Prevent principal Proteins receptor reduced Regulatory Replication reporter shown Spread supported target TBK1 TNF TRAF3 Treatment Type type I interferon upstream [DOI] 10.1371/journal.pone.0017192 PMC 바로가기 [Article Type] Research Article
In Vitro Reconstitution of SARS-Coronavirus mRNA Cap MethylationResearch Article Published on 2010-04-222022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 2003 Activation activity acute respiratory syndrome addition Author CAP cellular mRNA Complete complex coronavirus Coronaviruses CoV Critical drug design enzyme etiological agent event expression generate Genome host cell in viral in vitro inactive include induce infected cell Influenza virus inhibition assay initiated involved membrane-bound methylated Methylation methyltransferase mRNA mRNAs Mutation non-structural protein nsp14 predicted produced Protein replicate ribosomes RNA RNA genome RNA synthesis RNA transcript S-adenosyl-L-methionine SARS-coronavirus SARS-CoV SARS-CoV infected cells selective sequence target translation variety viral mRNAs viral nonstructural proteins Viral protein virus [DOI] 10.1371/journal.ppat.1000863 PMC 바로가기 [Article Type] Research Article