Crystal Structure of the C-Terminal Cytoplasmic Domain of Non-Structural Protein 4 from Mouse Hepatitis Virus A59Research Article Published on 2009-07-102022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Amino acid asymmetric unit C-terminal complex conformation conserved coronavirus Coronavirus replication crystal crystal structure cytoplasmic cytosol determined different dissociate disulfide bond domain Endoplasmic reticulum ER membrane expressed feature finding functional hydrophilic infected cells membrane membrane vesicle MHV monomer mouse mutant N- and C-termini non-structural proteins not affect Nsp3 nsp4 opposite physiological recruited reducing Region Replication Ser shown Structure wild-type [DOI] 10.1371/journal.pone.0006217 PMC 바로가기 [Article Type] Research Article
Studies on membrane topology, N-glycosylation and functionality of SARS-CoV membrane proteinResearch Published on 2009-06-182022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] acute respiratory syndrome Amino acid Analysis analysis biochemical C-terminal Cell culture conserved coronavirus Coronaviruses ectodomain endodomain ERGIC expressing found functional glycosylated Golgi complex immunofluorescence immunogenic Interaction M protein membrane membrane protein N-glycosylation N-glycosylation site N-terminal recruitment SARS-CoV SARS-CoV M Surface glycoprotein Topology transmembrane transmembrane domain Viral particles viral spike protein virion virus [DOI] 10.1186/1743-422X-6-79 PMC 바로가기 [Article Type] Research
The SARS-Unique Domain (SUD) of SARS Coronavirus Contains Two Macrodomains That Bind G-QuadruplexesResearch Article Published on 2009-05-152022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome ADP-ribose amino-acid Apoptosis Author bats bind binding coding compared component concerning coronavirus crystal structure determine determined different domain Evidence extreme form found function functional G-quadruplex gel electrophoresis Genome genomic RNA help homodimer host cell humans immune response in vitro include Infected involved lysine macrodomain mild infection monomer mRNA Mutation non-structural protein nucleic acid nucleotides nucleus occur oligonucleotide other coronaviruses outbreak pathogenicity Possible Protein protein 3 reduction Region regulate related virus remained Replication residue residues responsible RNA SARS Coronavirus SARS-CoV selected Seven shown Signal transduction Structure structures target three-dimensional structure Transcription viral infection viral replication virus X-ray crystallography [DOI] 10.1371/journal.ppat.1000428 PMC 바로가기 [Article Type] Research Article
Prevention of Cytotoxic T Cell Escape Using a Heteroclitic Subdominant Viral T Cell DeterminantResearch Article Published on 2008-10-242022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] alteration amino acid substitution analysis antigen-specific T cell association Author CD8+ T cell changes in clinical disease complex Complex molecule conformation Consensus Critical CTL cytotoxic determinant Disease progression Diseases elicit Enhancing epitope escape expressed functional Hepatitis histocompatibility HIV immune response immune responses immunodominant Infected Local Major memory MHC class I mice molecular basis motif mouse hepatitis mutated Mutation natural infection occur pathogen peptide performed prevention PROTECT Protective recombinant residue response significantly higher stability Subdominant T cell T cell epitope T cell response tumor immunity Vaccine development viral infection virus virus wildtype [DOI] 10.1371/journal.ppat.1000186 PMC 바로가기 [Article Type] Research Article
SARS-Coronavirus Replication/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In VitroResearch Article Published on 2008-05-022022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] activity acute respiratory syndrome addition Author cause centrifugation characteristic complex complexes connection Critical cytoplasm cytoplasmic Degradation dependent on develop digestion dissect eight enzyme Enzymes Factor fraction functional genomic RNA Host host cell membranes host cells in vitro in vitro activity Infected infected cell life-threatening membrane membrane structure membrane structures membranes non-structural protein NSP Nsps polyproteins precursor produced protease PROTECT protein synthesis released remained replicate Replication required RNA RNA synthesis robust RTC SARS-coronavirus SARS-CoV SARS-CoV-infected cells Structure subgenomic mRNA subgenomic mRNA synthesis subunit susceptible Transcription Treatment viral genome replication Viral RNA viral RNA synthesis virus-induced membrane structure virus-induced membrane structures [DOI] 10.1371/journal.ppat.1000054 PMC 바로가기 [Article Type] Research Article
Functional Genomics Highlights Differential Induction of Antiviral Pathways in the Lungs of SARS-CoV–Infected MacaquesResearch Article Published on 2007-08-102022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] activate activated acute phase acute respiratory distress acute respiratory syndrome Admission Adult against SARS-CoV analyzed Antiviral antiviral signaling Atypical Author cause Cell cells characterized chemokine genes chemokines circumvent Complication coronavirus Critical cynomolgus macaque cytokine demonstrated early IFN signaling Effect event examined functional gene expression profile gene signature Genomics highlight Host host response IFNs IL-8 immune response immunohistochemistry in vitro experiments individuals induction Infection innate immune response Innate immunity intensive care involved IP-10 lung Lung pathology macaque macaques nuclear translocation Pathogenesis pathogenic pathway phosphorylated Pneumonia protective immune response regulated required SARS patient SARS-CoV SARS-CoV infection SARS-CoV–infected cells severe shown Stat1 subset syndrome Transcription transcription factor type I interferon viral infection virus virus replication [DOI] 10.1371/journal.ppat.0030112 PMC 바로가기 [Article Type] Research Article
Type I feline coronavirus spike glycoprotein fails to recognize aminopeptidase N as a functional receptor on feline cell linesAnimal Published on 2007-06-012022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, [키워드] aminopeptidase N approach Cell culture cell line cell lines cellular receptor coronavirus coronavirus Coronavirus infection Coronaviruses Evidence expression functional identify in vivo Inconclusive Interaction less prevalent pseudotype receptor recognize replicate reported retroviral Sequence analysis serology spike glycoprotein Strains Surface glycoprotein to screen Type type I virus viruses [DOI] 10.1099/vir.0.82666-0 PMC 바로가기 [Article Type] Animal
Analysis of Intraviral Protein-Protein Interactions of the SARS Coronavirus ORFeomeResearch Article Published on 2007-05-232022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] acute respiratory syndrome Analysis Characteristics cloned complex coronavirus deleted different displaying expected expression function functional Genome genome-wide analysis growth in vitro interact Interaction interactions mammalian cells non-structural protein Nsp12 nsp13 nsp14 NSP2 Nsp9 open reading frames ORF Orf9b ORFeome pathogenicity performed positive predicted Protein protein interaction Proteins proteome Replication SARS-CoV shown stability tested Vero cells Viral proteins viral replication virus [DOI] 10.1371/journal.pone.0000459 PMC 바로가기 [Article Type] Research Article
Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virusResearch Published on 2007-02-262022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] acute respiratory syndrome aminopeptidase N arch BHK-21 cell canine Cell cells chick compared coronavirus coronavirus Coronaviruses Efficiency expression functional gastroenteritis GT HCoV-229E host cells host range human coronavirus 229E humans IBV identity Infected Infection infectious Infectious Bronchitis Virus Infectious disease Interaction kidney many cases mass Miguel notable receptor Result Strains suggested susceptibility susceptible TGEV transient transfection virus [DOI] 10.1186/1743-422X-4-20 PMC 바로가기 [Article Type] Research
The SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional CharacterizationResearch Published on 2005-08-252022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] ACE2 Alanine amino acid residue appear binding binding energy Cell characterization characterized cloned contribute decrease determined eight expressed expression functional Glutamine glycoprotein glycosylation glycosylation site identify immune responses include indicate initiated kDa lack Li mapping mechanism molecular weight Mutagenesis mutated Mutation not affect R426 RBD receptor receptor ACE2 reduced required residue residues S RBD SARS Coronavirus significantly Simultaneous submitted suggested the RBD the receptor-binding domain These data [DOI] 10.1186/1743-422X-2-73 PMC 바로가기 [Article Type] Research