Performance and clinical validation of the RealStar ® MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA중동 호흡기 증후군 코로나바이러스 RNA 검출을 위한 RealStar ® MERS-CoV 키트의 성능 및 임상 검증Article Published on 2014-03-282022-09-25 Journal: Journal of Clinical Virology [Category] MERS, 진단, [키워드] Altona Diagnostics amplification analytical sensitivity assays clinical sample comparable coronavirus cross-reactivity Diagnosis diagnostic assay Diagnostic method envelope Epidemiology Frame genomic region Germany Hamburg highly pathogenic IMPROVE Internal control management MERS-CoV MERS-CoV infection MERS-CoV RNA Middle East Middle East respiratory syndrome molecular Munich nucleic acid objective ORF Patient PCR performance real-time RT-PCR Respiratory disease respiratory diseases respiratory pathogen respiratory sample respiratory syndrome coronavirus Result RNA RT-PCR assay RT-PCR kit target region upstream virus WHO World Health Organization [DOI] 10.1016/j.jcv.2014.03.012 PMC 바로가기 [Article Type] Article
Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory PathogensResearch Article Published on 2012-11-142022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Acute respiratory infection approaches ARI collected compared Concentration conducted detected detection diagnostic DNA DNA concentration Epidemiology equivalence finding Germany Human human coronavirus OC43 multiplex nasal swab nasal swabs nostril participant Participants pathogen pathogen detection Pathogens Prospective Study Quality control reported respiratory respiratory pathogen respiratory viral rhinovirus RT-PCR self Self-swab subsequent Swab swabbing swabs symptomatic Symptoms viral respiratory was determined Weekly β-actin [DOI] 10.1371/journal.pone.0048508 PMC 바로가기 [Article Type] Research Article
Human airway epithelial cell culture to identify new respiratory viruses: Coronavirus NL63 as a modelArticle Published on 2008-12-052022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] airway amplification amplify antibody approach approaches Cell culture cell line clinical samples coronavirus CPE, cytopathic effect Culture culture supernatant cultures Cytopathic effect described detect determine different time point epithelial evaluated experiment HAE HAE culture HAE, human airway epithelium HCoV-NL63 HCoV-NL63, human coronavirus NL63 Human identify Infection lack molecular NL63 nucleic acid pathogen Pathogens Propagation reagent reported respiratory pathogen respiratory viral respiratory virus SARS-CoV, severe acute respiratory syndrome coronavirus secretion sequence Sequence analysis TEM TEM, transmission electron microscopy Transcriptome transmission electron microscopy variety virus Whole transcriptome amplification WTA, whole transcriptome amplification [DOI] 10.1016/j.jviromet.2008.10.022 PMC 바로가기 [Article Type] Article
Detection and typing by molecular techniques of respiratory viruses in children hospitalized for acute respiratory infection in Rome, ItalyResearch Article Published on 2007-02-202022-11-01 Journal: Journal of medical virology [Category] Coronavirus, Fulltext, MERS, [키워드] Acute respiratory infection adopted amplified fragment approach assay Asthma Bronchiolitis bronchitis bronchopneumonia children circulation Co‐infection Department described detected detection Diagnosis enrolled Hospitalized Inc infected children Infection influenza type A involved Italy métapneumovirus molecular NL63 Novel coronavirus parainfluenza virus pathogen Patient Pneumonia positive result respiratory pathogen respiratory syncytial virus respiratory tract infection respiratory virus reverse transcription rhinovirus Rome RSV RT‐PCR sequenced the patient to define viral pathogen virus [DOI] 10.1002/jmv.20832 PMC 바로가기 [Article Type] Research Article
SARS and Common Viral InfectionsDispatch Published on 2004-06-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome Adenovirus Chlamydia common Influenza métapneumovirus molecular molecular testing Mycoplasma pneumoniae parainfluenza virus Patient polymerase chain reaction respiratory pathogen respiratory syncytial virus SARS Severe acute respiratory syndrome [DOI] 10.3201/eid1006.030863 PMC 바로가기 [Article Type] Dispatch