Diagnosis of infection by SARS-COV-2: Is one molecular test enough?SARS-COV-2 감염 진단: 분자 검사 하나로 충분합니까?Article Published on 2021-05-012022-09-12 Journal: European journal of internal medicine [Category] MERS, SARS, 진단, [키워드] Abstract coronavirus COVID-19 Diagnosis Infection molecular test Pandemia Real-time PCR SARS-CoV-2 [DOI] 10.1016/j.ejim.2021.02.005 PMC 바로가기 [Article Type] Article
Evaluation of mobile real-time polymerase chain reaction tests for the detection of severe acute respiratory syndrome coronavirus 2중증급성호흡기증후군 코로나바이러스 2 검출을 위한 모바일 실시간 중합효소연쇄반응 검사 평가Article Published on 2021-04-302022-09-10 Journal: Scientific Reports [Category] MERS, SARS, 치료기술, 치료제, [키워드] 5′ UTR acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Bacterial pathogens Biomonitoring can be used Care caused Cell culture clinical sample clinical samples Combination coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cross-reactivity detect Diagnosis diarrhea epidemic diarrhea evaluated gastroenteritis Genes genetic material handling Health Health care human respiratory virus IBV inactivated independent Infectious Bronchitis Virus Laboratory limit lysis buffer MERS MERS-CoV Microbiology middle Middle Eastern respiratory syndrome coronavirus molecular assays Molecular biology operator ORF1ab pandemic Pathogens PEDV platform point-of-care polymerase chain PrEP reaction Real-time PCR respiratory respiratory syndrome coronavirus respiratory viruses RNA room temperature Safe Sample SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 test SARS-CoV-2-infected hamsters severe acute respiratory syndrome Coronavirus Swab swabs target targets temperature test results Transmission untranslated region Viral viral infection virus viruses [DOI] 10.1038/s41598-021-88625-6 PMC 바로가기 [Article Type] Article
One-step differential detection of Salmonella enterica serovar Typhi, serovar Paratyphi A and other Salmonella spp. by using a quadruplex real-time PCR assayarticle Published on 2021-04-302024-09-01 Journal: Journal of Microbiological Methods [Category] 살모넬라증, [키워드] Real-time PCR S. enterica serovar Paratyphi A S. enterica serovar Typhi Salmonellae detection [Article Type] article
Natural tannin extracts supplementation for COVID-19 patients (TanCOVID): a structured summary of a study protocol for a randomized controlled trialCOVID-19 환자를 위한 천연 탄닌 추출물 보충제(TanCOVID): 무작위 대조 시험을 위한 연구 프로토콜의 구조화된 요약Letter Published on 2021-04-282022-09-10 Journal: Trials [Category] MERS, SARS, 임상, [키워드] 1:1 24 hour 24 hours addition Administered administration affected age All trial participants all-cause mortality Alpha alpha error Antibiotic therapy antipyretics assigned assisted ventilation Azithromycin B12 blinded Blinding branch Buenos Aire Buenos Aires can not Chestnut tannins Clinical symptoms clinical trial closure cognitive computer-generated random numbering conditions Consent control group COVID-19 COVID-19 patient criteria cytokine Cytokines Day Decline decrease defined Definition Department described diagnosis of SARS-CoV-2 dietary supplement difference disease dissemination dose double-blind Efficacy element eligibility eligible enrolled Enrollment enrolment epidemiological evaluate excluded fecal Final first dose first visit Gender gut microbiota Hospital de Clínica Hospital de Clínicas Hospital de Clinicas Jose de San Martin hospital discharge Hydroxychloroquine hypersensitivity ICU impaired consciousness increase in indicated Infection Infectious disease Infectious diseases Inflammation inflammatory mediator Inflammatory mediators Informed consent intestinal intestinal permeability invasive ventilation lactating Level Lopinavir mean difference measure mechanical ventilation mediators microbiota modifications molecular natural non-invasive ventilation number objective Older outcome parallel-group parameter participant Participation Patient patients per day Placebo placebo-controlled platform Potential power power of 80% protocol Quebracho tannins randomization Randomized Randomized controlled trial Randomly Real-time PCR receive recruited recruitment Registered Research Ritonavir Sample size SARS-COV-2 infection SARS-CoV2 screened secondary secondary outcome measures severity Standard therapy standard treatment status study participant Study protocol study randomization submitted supplementary material supply tannin tannins therapy time Time frame took place Treatment treatment allocation Trial trial participant Trial registration undergo university Ventilation website [DOI] 10.1186/s13063-021-05281-x PMC 바로가기 [Article Type] Letter
Repositive RT-PCR test in discharged COVID-19 patients during medical isolation observation격리 관찰 중 퇴원한 코로나19 환자에서 RT-PCR 양성 반응Research Paper Published on 2021-04-262022-09-12 Journal: International Journal of Medical Sciences [Category] MERS, SARS, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antibody Clinical characteristics clinical feature Clinical features collected conducted coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus disease 2019 (COVID-19) COVID-19 COVID-19 patient determinant discharge discharged discharged patient discharged patients epidemiological Hospitalization IgG and IgM individual information Isolation management observation Patient patients performed positive Prevalence provide quarantine Real-time PCR Repositive Retrospective study RNA RT-PCR test SARS-CoV-2 severe acute respiratory syndrome Coronavirus symptomatic viral shedding with COVID-19 [DOI] 10.7150/ijms.58766 PMC 바로가기 [Article Type] Research Paper
Versatile and flexible microfluidic qPCR test for high-throughput SARS-CoV-2 and cellular response detection in nasopharyngeal swab samples고처리량 SARS-CoV-2 및 비인두 면봉 샘플의 세포 반응 검출을 위한 다목적이고 유연한 미세유체 qPCR 테스트Research Article Published on 2021-04-142022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] absence buffer bypassing cellular response clinical sample clinical samples compatibility composed contribute diagnostic diagnostic test evaluated flexible Host IMPROVE large sample limitations lysis buffer MicroRNAs nasopharyngeal swab sample offer overcome pathogen Pathogens Patient PCR reaction Population presence or absence probe proteinase Proteinase K protocols qPCR qPCR test Quantitative reaction Real-time PCR receptors reference method reverse transcription RNA extraction RNA purification RT-qPCR SARS-CoV-2 SARS-CoV-2 variants Spread test results the SARS-CoV-2 this system time-consuming virus Volume [DOI] 10.1371/journal.pone.0243333 PMC 바로가기 [Article Type] Research Article
Low risk of SARS-CoV-2 in blood transfusion수혈 시 SARS-CoV-2의 낮은 위험Research Article Published on 2021-04-132022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 age all subject all subjects Asymptomatic Blood Blood donors Blood Transfusion caused China classical clinically collected conducted Contact convalescent coronavirus disease Coronavirus-2 cough COVID-19 criteria cross-sectional Cycling determine diagnostics discharge evaluate Evidence evidence of genomic region hematogenous individual individuals infected individual infected individuals KNUST LBP male Medicine minimum Novel coronavirus novel coronavirus disease nucleic acid nucleic acid extraction ORF1ab pandemic Patient PCR plasma populations positive potential risk predominant Prevalence Real-time PCR recovered patients recruited reported respiratory Result Retrospective study risk risk of COVID-19 Saliva SARS-CoV-2 SARS-CoV-2 testing SARS-CoV-2 viral RNA SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sputum Sputum sample subject subjects Symptom symptomatic symptoms of COVID-19 tested threshold Transmission transmission of SARS-CoV-2 transmitted transported tropical medicine Viral viral nucleic acid Viremia virus was performed [DOI] 10.1371/journal.pone.0249069 PMC 바로가기 [Article Type] Research Article
Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational StudyCommunication Published on 2021-04-102022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] 2019-nCoV absence airway antibody Autopsy cause chemiluminescence death Deceased detectable detection diagnosis of SARS-CoV-2 died different time point evaluated IgM/IgG management medico-legal procedures pandemic persistence positive positive individual Post-mortem Real-time PCR RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 positive patient SARS-CoV-2 viral RNA subject Swab Viral load virus was performed [DOI] 10.3390/microorganisms9040800 PMC 바로가기 [Article Type] Communication
Acute and post-acute phase of COVID-19: Analyzing expression patterns of miRNA-29a-3p, 146a-3p, 155-5p, and let-7b-3p in PBMCComparative Study Published on 2021-04-062022-10-04 Journal: International immunopharmacology [Category] MERS, 바이오마커, [키워드] acute COVID-19 acute respiratory syndrome addition Analysis appear assays Biomarker cellular miRNA characteristic coronavirus coronavirus disease COVID-19 COVID-19 diagnosis COVID-19 disease COVID-19 patient COVID-19 patients COVID‐19 demonstrated Diagnosis diagnosis and monitoring disease evaluated expression level expression pattern groups healthy information microRNA miRNA miRNA expression miRNA profile mononuclear cell pathogen PBMC PBMCs Peripheral blood Real-time PCR Result ROC ROC analysis SARS-CoV-2 selected sensitivity significantly significantly higher specificity suggested the disease the healthy was tested [DOI] 10.1016/j.intimp.2021.107641 PMC 바로가기 [Article Type] Comparative Study
Evaluation of seven commercial RT-PCR kits for COVID-19 testing in pooled clinical specimensObservational Study Published on 2021-04-012023-06-19 Journal: Journal of medical virology [Category] MERS, SARS, [키워드] commercial kits COVID-19 Real-time PCR Sample pooling World Health Organization [DOI] 10.1002/jmv.26691 PMC 바로가기 [Article Type] Observational Study