Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infectionArticle Published on 2012-11-192022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] Adults antibody Antigen antigenicity Betacoronavirus Blood blood sample C-terminal domain characterisation Coronavirus infection cross-react demonstrated detected antibody Diagnosis diagnostic different E, Envelope Elderly patient epidemiological evaluated greater HCoV-229E HCoV-OC43 HCoV-OC43, human coronavirus OC43 Human coronavirus human coronavirus OC43 human CoV human serum humans identify immune response immunoreactivity Infection IPTG, β-d-1-thiogalactopyranoside M, matrix N-terminal domain NP, nucleocapsid protein nucleocapsid protein OC43 strain occur offer patients polyclonal polyclonal antibody previous study produced Protein purified rabbit polyclonal antibody reactivity Region reported respiratory infection RNP, ribonucleoprotein S, spike SARS-CoV serum serum sample specificity SPR, surface plasmon resonance Strong tested Variability virus infection [DOI] 10.1016/j.jviromet.2012.11.009 PMC 바로가기 [Article Type] Article
Etiology and Clinical Characterization of Respiratory Virus Infections in Adult Patients Attending an Emergency Department in BeijingResearch Article Published on 2012-02-282022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] addressed Adult assay Attending Beijing characterization checked China clinical clinical symptom conducted Department detected determine different dominant effective Emergency epidemiological finding HCoV-229E Health highlight human coronavirus influenza virus métapneumovirus nose parainfluenza pathogen Patient picornavirus polymerase chain positive Prevalence profile real-time RT-PCR reported respiratory respiratory syncytial virus respiratory tract infection respiratory virus Respiratory virus infection respiratory viruses reverse transcription RSV Safe significantly tested therapeutic approach throat swab virus virus virus infection viruses [DOI] 10.1371/journal.pone.0032174 PMC 바로가기 [Article Type] Research Article
A Genome-Wide Screen for Interactions Reveals a New Locus on 4p15 Modifying the Effect of Waist-to-Hip Ratio on Total Cholesterol상호 작용을 위한 게놈 와이드 스크린은 총 콜레스테롤에 대한 허리 대 엉덩이 비율의 영향을 수정하는 4p15에 대한 새로운 위치를 밝힙니다.Research Article Published on 2011-10-202022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] A allele adipose Adipose tissue affected affecting allele approach association Author body composition candidate gene cardiovascular diseases CCKAR characterized cholesterol chromosome circulating Cohort collected Concentration conducted contribute dataset demonstrated described differential expression distribution Effect enrichment epidemiological European explain found functions G allele Gene Gene Expression Genetic variant genome-wide association genome-wide significant genomic Genotype genotypes global health GWA Health heritability homozygous identify individual initial Interaction Intervention joint Lipid lipid metabolism lipids loci locus Low-density lipoprotein Low-Density Lipoprotein cholesterol measure Meta-analysis metabolism modify Modifying New open PCDH7 Phenotypes population-based cohort profiles Ratio recent related Replication reported risk risk factor Risk factors Screen screened serum serum lipid Serum lipids Sex Standard deviation tissue Total Total cholesterol Triglyceride triglycerides variant variants waist waist-to-hip ratio WHR [DOI] 10.1371/journal.pgen.1002333 PMC 바로가기 [Article Type] Research Article
Temporal Variability and Social Heterogeneity in Disease Transmission: The Case of SARS in Hong KongResearch Article Published on 2009-08-212022-10-28 Journal: PLoS Computational Biology [Category] Coronavirus, MERS, [키워드] acute respiratory syndrome Affect applied Author average behavioural Better case change changes in Community Contact Course Critical different disease transmission effective Epidemic Epidemics epidemiological estimate event Evolution example functions General population healthcare healthcare worker Hong Kong hospital Hospitalized hospitals infect infectious influenza pandemic Markov chain mean measure observation outbreak parameter parameters Probability PROTECT provided public health measure recent reduced remained Reproduction number risk of infection SEIR shown subpopulation susceptible-exposed-infectious-removed sustained Temporal the SARS epidemic Transmission transmission rates was used were infected [DOI] 10.1371/journal.pcbi.1000471 PMC 바로가기 [Article Type] Research Article
Co-circulation of human metapneumovirus and SARS-associated coronavirus during a major nosocomial SARS outbreak in Hong KongArticle Published on 2007-11-012022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, MERS, [키워드] 2003 2003 SARS 95% CI clinical manifestation Co-infection collected coronavirus cough detected determine Epidemic epidemiological Factor HCW health-care Hong Kong human metapneumovirus independent independent risk factors individual Infection information Logistic regression analysis métapneumovirus multivariate nasopharyngeal nosocomial nosocomial outbreak not different objective occurred Other outbreak outcome Patient patients Result risk factor RNA RT-PCR assay SARS SARS epidemic SARS infection SARS patients statistically significant tested was performed was used [DOI] 10.1016/j.jcv.2007.08.015 PMC 바로가기 [Article Type] Article
Human respiratory coronavirus HKU1 versus other coronavirus infections in Italian hospitalised patientsArticle Published on 2007-01-102022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, [키워드] 229E Acute respiratory tract infections Analysis ARTI, acute respiratory tract infections BAL, bronchoalveolar lavage caused clinical symptom compared contribute Coronavirus infection detected DFA, direct fluorescent antibody staining Diagnosis diagnostic epidemiological feature found Genotype HCoV HCoVs hCoVs, human coronavirus HKU1 HKU1 genotypes Hong Kong hospitalised hospitalised patient Human Human coronavirus HKU1 identify Infected Infection infections Italian Italy longitudinal MAbs, monoclonal antibodies monoclonal antibody nine NPA, nasopharyngeal aspirate objective Patient patient population patients performed predominant Prospective Study reported Respiratory Coronavirus respiratory tract infection Result reverse transcription-PCR rhinorrhea RT-PCR RT-PCR, reverse transcription-PCR significantly lower strain SVC, shell-vial cultures [DOI] 10.1016/j.jcv.2006.12.008 PMC 바로가기 [Article Type] Article
Adaptive evolution of the spike gene of SARS coronavirus: changes in positively selected sites in different epidemic groupsResearch Article Published on 2006-10-042022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] acute respiratory syndrome adaptive addition Amino acid changes in characterized contribute coronavirus Course CoV detected different domain Epidemic epidemiological Evolution found greater group groups heptad repeat hosts human host humans membrane fusion occurred outbreak Phylogenetic tree positive Positive selection proportion Protein RBD receptor residue Result reveal S gene S protein SARS-CoV selected selective selective pressure selective sweep spike gene strain subsequent the S protein Transmission two group two groups Variation viral adaptation [DOI] 10.1186/1471-2180-6-88 PMC 바로가기 [Article Type] Research Article
Public health implications of using various case definitions in The Netherlands during the worldwide SARS outbreakOriginal Articles Published on 2006-08-072022-11-01 Journal: Clinical microbiology and infection : the official [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome analysed assessment Case definition caused Changing Confusion consequence criteria deviation different epidemiological FIVE Health implication Influenza management Measures Netherland outbreak pandemic Patient Perspective Public public health public health threats reported required resources risk Risk assessment SARS Sensitivity and specificity serological the patient the WHO Threats Virological were used [DOI] 10.1111/j.1469-0691.2006.01552.x PMC 바로가기 [Article Type] Original Articles
A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation studyResearch Article Published on 2005-10-182022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, [키워드] accurate allelic Analysis approach assay can be used carried caused characteristic clinical sample Cohort different Epidemic epidemiological epidemiologically Epidemiology Genotype genotypes Infection Infectious disease information isolate MONITOR outbreak Patient probe recruited related reported respiratory Result SARS patient SARS-coronavirus Sequencing Sequencing analysis severe TaqMan the epidemic viral evolution [DOI] 10.1186/1471-2334-5-87 PMC 바로가기 [Article Type] Research Article
Severe acute respiratory syndrome (SARS)—paradigm of an emerging viral infection ☆Article Published on 2003-11-262022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome After Antiviral compound Antiviral treatment approach approaches carried caused China Complete consequence coronavirus country criteria deaths diagnostic test different East effort emerging infection epidemiological Epidemiology Evidence example found Genome globe group Human inactivation Infectious disease International Laboratory laboratory diagnosis molecular virology outbreak Research RNA SARS patients SARS-associated coronavirus (SARS-CoV) SARS-CoV sequenced severe Severe acute respiratory syndrome (SARS) severe respiratory illness spread to stability starting Transmission Vaccine viral infection virus WHO World Health Organization World Health Organization (WHO) [DOI] 10.1016/j.jcv.2003.09.011 PMC 바로가기 [Article Type] Article