In silico identification of conserved cis -acting RNA elements in the SARS-CoV-2 genomeShort Communication Published on 2020-07-292022-10-31 Journal: Future Virology [Category] COVID-19, [키워드] cis-acting RNA elements 3′ end acute respiratory syndrome Analysis Antiviral conserved coronavirus element Genome genomic material ORF1ab predict RNA RNA secondary structure RNA sequence RNA structure RNA virus SARS-CoV SARS-CoV-2 Structure the SARS-CoV-2 the SARS-CoV-2 genome therapeutic Vaccine viral sequence were used [DOI] 10.2217/fvl-2020-0163 PMC 바로가기 [Article Type] Short Communication
Development of a triplex real-time RT-PCR assay for detection and differentiation of three US genotypes of porcine hemagglutinating encephalomyelitis virusArticle Published on 2019-04-052022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] 3′ end Analysis cause clinical manifestation clinical sample clinical samples conserved Critical cross reaction Deletion detect detection Detection limit development differentiation DNA DNA sequencing Evidence Evolution Genotype genotyped genotypes MONITOR mutant NS2 nucleocapsid Older PHEV positive positive-sense RNA virus primer probe probes provide real time real time RT-PCR real-time RT-PCR recent finding Region representing Respiratory disease RT-PCR RT-PCR assay sensitivity sets of primer single-stranded specificity strain virus viruses vomiting [DOI] 10.1016/j.jviromet.2019.04.008 PMC 바로가기 [Article Type] Article
Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirusShort Communication Published on 2017-02-132022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, [키워드] 3′ end accessory protein Amino acid Analysis Cell cell lysate coronavirus demonstrated ectopic expression ENCODE ENhance expression indirect immunofluorescence kDa mAb NS7a Porcine deltacoronavirus predict predicted Protein purified subgenomic mRNA subgenomic RNA suggested transcription regulatory sequence transfected with [DOI] 10.1099/jgv.0.000690 PMC 바로가기 [Article Type] Short Communication
Recombinant Canine Coronaviruses in Dogs, EuropeResearch Published on 2010-01-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] 3′ end canine Canine coronavirus coronavirus detection rate distribution Europe Fecal sample found gastroenteritis genetic analysis Genotype Greece highest Hungary IIb Infection lowest phylogenetic analysis positive Prevalence recombinant recombinant strains Recombination related Research strain subtype tested TGEV the spike protein virus [DOI] 10.3201/eid1601.090726 PMC 바로가기 [Article Type] Research
Specific real-time reverse transcription-polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in tissues and feces from turkeys infected with turkey coronavirusArticle Published on 2009-11-142022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] 3′ end acute enteritis amplified antibody assays average Bacteria base-pair cause collected conducted coronavirus coronavirus RNA Critical detected determine Diagnosis different DPI economic loss FAM fecal Fecal sample fluorescent highest IFA immunofluorescence indicated Infected Infection inoculated interval intestine Other PCR primer positive primer probe progression quantitation Rapid reached real-time RT-PCR reporter RNA sensitivity serial dilution Specific specificity spike gene Swab swabs TaqMan probe the disease tissue Trial trials Turkey Turkey coronavirus viral genome Viral RNA viral RNA load virus was performed [DOI] 10.1016/j.jviromet.2009.11.012 PMC 바로가기 [Article Type] Article
The Structure of a Rigorously Conserved RNA Element within the SARS Virus GenomeResearch Article Published on 2005-01-282022-10-28 Journal: PLoS Biology [Category] Coronavirus, [키워드] 3′ end absence Anti-viral approach bind Comparisons component conserved coronavirus element explained extreme feature function Genome homology Host host cell host protein Human genome in viral include Interaction interactions interior ions macromolecular mechanism motif mutable occur pathogen pathogenic Protein protein synthesis reactive related required ribosomal RNA RNA RNA genome RNA genomes SARS virus Sequence conservation Structure target therapeutic agents unique Viral RNA virus [DOI] 10.1371/journal.pbio.0030005 PMC 바로가기 [Article Type] Research Article