Lateral flow–based nucleic acid detection of SARS-CoV-2 using enzymatic incorporation of biotin-labeled dUTP for POCT usePOCT 사용을위한 비오틴-표지 된 DUTP의 효소 혼입을 사용하여 SARS-COV-2의 측면 유동 기반 핵산 검출Communication Published on 2022-01-192022-09-01 Journal: Analytical and bioanalytical chemistry [Category] COVID19(2023년), Fulltext, SARS, 진단, 치료기술, [키워드] Abstract Accuracy Biotin cDNA Combination COVID-19 COVID-19 pandemic Data analysis detrimental effect detrimental effects develop diagnostic effort FITC immunochromatographic incorporation indicate isothermal isothermal amplification LAMP Lateral lateral flow Lateral flow assay (LFA) Loop-mediated isothermal amplification N-gene nucleic acid nucleic acid amplification Nucleic acid detection overcome Pandemics Pathogens POCT point of care Point of care testing (POCT) positive primer Rapid reduced Research research and development reverse transcriptase Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RNA sample RNA samples RT-LAMP SARS-CoV-2 SARS-CoV-2 viral RNA SARS-CoV-2 N-gene semi-quantitative sensitive sensitivity Specific specificity supplementary material tested Viral Viral RNA [DOI] 10.1007/s00216-022-03880-4 PMC 바로가기 [Article Type] Communication
Analytical and Clinical Evaluation of the Semiquantitative Elecsys Anti-SARS-CoV-2 Spike Protein Receptor Binding Domain Antibody Assay on the Roche cobas e602 AnalyzerRoche cobas e602 분석기에서 반정량적 Elecsys Anti-SARS-CoV-2 스파이크 단백질 수용체 결합 도메인 항체 분석의 분석 및 임상 평가Article Published on 2022-01-062022-09-11 Journal: American journal of clinical pathology [Category] SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analyzer Anti-spike anti-spike antibodies antibodies antibody Bilirubin Biotin clinical clinically Concordance coronavirus COVID-19 COVID-19 patient COVID-19 vaccination cross-reactivity detect diagnostics Elecsy ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluate evaluated exhibit greater hemoglobin IgA antibodies IgA antibody IgG IgM immunoassay Interference Lateral flow device linearity measure mRNA Neutralization assay PCR positivity plasma Precision Protein Receptor binding domain respiratory robust Roche SAR-CoV-2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein SARS-CoV-2 virus Semiquantitative semiquantitatively Sensitivity and specificity serology Seropositivity serum spike Spike protein triglycerides turn [DOI] 10.1093/ajcp/aqab092 PMC 바로가기 [Article Type] Article
The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entrySARS-COV-2 스파이크 (S)와 ZDHHC5/GOLGA7의 상호 작용 및 S 단백질의 세포 내 위치, 팔미 토일 화 및 슈도 바이러스 진입에 미치는 영향Research Published on 2021-12-272022-09-10 Journal: Virology Journal [Category] SARS, 치료기술, [키워드] A549 acute respiratory syndrome acute respiratory syndrome coronavirus Affect affected Affinity purification analyzed Antiviral APT2 assays Biotin Cas9 Cell cell line cell lines cell types cells co-immunoprecipitation co-IP Colocalization complex coronavirus CRISPR CRISPR-Cas9 cysteine cysteine residue determine domain Effect Efficiency endodomain enzymatic activity enzyme fluorescence microscopy GOLGA7 had no HEK293T HEK293T cell HEK293T cells Hela HeLa cells host protein host proteins independent independent of interact Interaction knowledge mass spectrometry no significant effect palmitoylation Protein provide pseudovirus entry reason Renilla Renilla luciferase respiratory Result S protein S proteins SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 S protein SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant effect significantly Spike protein subcellular localization the cell the S protein virus entry virus infection Virus-host interaction ZDHHC5 ZDHHC5/GOLGA7 [DOI] 10.1186/s12985-021-01722-w PMC 바로가기 [Article Type] Research
Detection of cross-reactive immunoglobulin A against the severe acute respiratory syndrome-coronavirus-2 spike 1 subunit in saliva중증 급성 호흡기 증후군-코로나 바이러스 -2 스파이크에 대한 교차 반응성 면역 글로불린 A의 검출 1 타액의 서브 유닛Research Article Published on 2021-11-232022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료기술, [키워드] age analyzed angiotensin angiotensin converting enzyme Angiotensin converting enzyme-2 binding Biotin blood sample Blood samples breast milk Chain Reaction correlated cross-react cross-reaction cross-reactive cross-reactivity dental dentist dentists detection determined by enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay help IgG and IgM Immunity immunochromatography Immunoglobulin immunoglobulin A individual Infection involved men mucosal surfaces Mucus Patient patients PCR PCR-negative polymerase chain polymerase chain reaction Prevent proportion Protein provide purified reaction receptor Receptor-binding domain reported Saliva saliva samples SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein secretory immunoglobulin A SIgA significantly lower Spike protein subunit suppress the receptor-binding domain university virus was determined women [DOI] 10.1371/journal.pone.0249979 PMC 바로가기 [Article Type] Research Article
Catalytic hairpin DNA assembly-based chemiluminescent assay for the detection of short SARS-CoV-2 target cDNA짧은 SARS-CoV-2 표적 cDNA 검출을 위한 촉매 헤어핀 DNA 어셈블리 기반 화학발광 분석Article Published on 2021-10-012022-09-11 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplify approach Biotin biotinylated catalytic Catalytic hairpin assembly cDNA challenge Chemiluminescent assay color change colorimetric detection Combination complex Concentration detect Detection limit Detection sensitivity diagnostics dimeric DNA DNA probe enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay equipment exhibit horseradish peroxidase IMPROVE interactions mutant mutant gene naked eye peroxidase powerful tool probe protocol resulting SARS-CoV-2 selective sensitivity sequence specificity target Target DNA. target gene Target genes [DOI] 10.1016/j.talanta.2021.122505 PMC 바로가기 [Article Type] Article
Multiplexed Magnetofluorescent Bioplatform for the Sensitive Detection of SARS-CoV-2 Viral RNA without Nucleic Acid Amplification핵산 증폭 없이 SARS-CoV-2 바이러스 RNA의 민감한 검출을 위한 다중 자기형광 바이오플랫폼Article Published on 2021-08-172022-09-11 Journal: Analytical Chemistry [Category] SARS, 치료기술, 치료제, [키워드] acid Biotin clinical sample clinical samples complementary conditions COVID-19 pandemic death detect detection dynamic range fluorescent Genes genetic material gold gold standard help Hepatitis hepatitis C horseradish peroxidase linear loci magnetic bead Magnetic beads measles mitigate N genes Nasopharyngeal swab nasopharyngeal swabs Nile ORF1a outbreak Oxidation Patient patients peroxidase phenylenediamine point of care probe Rapid reached related virus reporter RNA RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV-2 virus sensitive sensitivity Sensitivity and specificity subjects the SARS-CoV-2 total RNA Viral RNA viruses were used West [DOI] 10.1021/acs.analchem.1c01950 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Envelope (E) protein interacts with PDZ-domain-2 of host tight junction protein ZO1SARS-COV-2 엔벨로프 (E) 단백질은 숙주 단단한 접합 단백질 ZO1의 PDZ- 도메인 -2와 상호 작용한다.Research Article Published on 2021-06-092022-08-13 Journal: PLoS ONE [Category] Biochemistry, MERS, SARS, 변종, 진단, [키워드] airway amplified approach approaches array binding Biotin C-terminal cell layer chloride complementary consolidation contribute contributing to COVID-19 patients disease severity domain dysfunction E protein ECT envelope epithelial epithelial cell epithelial cells epithelial tissues extreme Fluorescence fusion protein global pandemic homogenous Host HTRF humans interact Interaction Intervention lead Lungs membrane protein mitigate molecular morbidity motif multifunctional mutant Pathogenesis Pathologies peptide Protein reduce respiratory Respiratory disease respiratory tract SARS-CoV-1 SARS-CoV-2 screened secretion sequence severe pneumonia severe respiratory disease singular target the lungs a the SARS-CoV-2 tight junction tissues virus virus spread Zona [DOI] 10.1371/journal.pone.0251955 PMC 바로가기 [Article Type] Research Article
Biomedical Text Link Prediction for Drug Discovery: A Case Study with COVID-19Article Published on 2021-05-262022-10-28 Journal: Pharmaceutics [Category] COVID-19, [키워드] Accuracy acute respiratory syndrome Algorithm Anti-inflammatory antiviral therapy Artemisinin Biotin Bone marrow calculated Chloroquine complement complex Complex networks coronavirus Coronaviruses COVID-19 data sets Diseases drug drug candidate Drug discovery Efficacy envelope proteins heterogeneous identify include inhibitors interferon knowledge leukocyte literature review machine learning MERS methotrexate natural language processing nucleoside analogs occur outbreak predict predicted prediction protease inhibitors Proteins Repurposed drug Repurposed drugs SARS Coronavirus SARS-CoV-2 SARS-CoV-2 treatment Side effects Suramin syndrome text text mining Therapies treat vaccination with COVID-19 [DOI] 10.3390/pharmaceutics13060794 PMC 바로가기 [Article Type] Article
A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis밀도차에 의한 회전집속유동(RFF) 미세유체 바이오센서로 조기 진단 가능Article Published on 2021-04-292022-09-10 Journal: Scientific Reports [Category] MERS, SARS, 바이오마커, 진단, 치료기술, 치료제, [키워드] Biomarker Biotin CFD Concentration COVID-19 demonstrated detectable Diagnosis ENhance Flow fluorescent focus less magnitude Mechanical engineering mechanism Modification optical pandemic platform point-of-care Probability provide sensitivity Sensors and biosensors silicon suggested [DOI] 10.1038/s41598-021-88647-0 PMC 바로가기 [Article Type] Article
Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2Vigilant: SARS-CoV2의 측면 흐름 분석 기반 검출을 위한 엔지니어링된 VirD2-Cas9 복합체Letter Published on 2021-04-122022-09-10 Journal: Nano Letters [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] amplification amplified bind biosensors Biotin biotinylated catalytically clinical samples comparable COVID-19 CRISPR CRISPR-Cas9 cross-reactivity dCas9 detect detection disease disease control exhibited FAM Flow fusion inactive lateral flow assay Lateral limit of detection MERS molecular diagnostics nucleic acid Nucleic acid detection offer pathogen Pathogens platform point-of-care polymerase Rapid recombinase polymerase amplification relaxases robust RT-RPA SARS-CoV-2 SARS-CoV2 Sensitivity and specificity sequence this system VirD2 [DOI] 10.1021/acs.nanolett.1c00612 PMC 바로가기 [Article Type] Letter