Eosinophilic esophagitis auxiliary diagnosis based on a peptide ligand to eosinophil cationic protein in esophageal mucus of pediatric patients Research Published on 2022-07-182022-10-05 Journal: Scientific Reports [Category] 바이오마커, [키워드] allergic Analysis association atopic auxiliary biopsy cationic characterized Clinical practice clone demonstrated develop Diagnosis disease disorder Dysphagia eosinophil Eosinophilic eosinophils esophagitis evaluated fibrosis Gastroesophageal reflux histopathological individual individuals inflammatory condition Ligand mass spectrometry Patient patients Pediatric patient Pediatric patients peptide performed Protein proteomic reactive reactivity responses selected sensitivity specificity tissue up-regulated was used [DOI] 10.1038/s41598-022-16293-1 [Article Type] Research
Longitudinal Assessment of SARS-CoV-2-Specific T Cell Cytokine-Producing Responses for 1 Year Reveals Persistence of Multicytokine Proliferative Responses, with Greater Immunity Associated with Disease Severity1년 동안 SARS-CoV-2-특이적 T 세포 사이토카인 생성 반응에 대한 종단 평가는 질병 중증도와 관련된 더 큰 면역과 함께 다중 사이토카인 증식 반응의 지속성을 보여줍니다Article Published on 2022-07-132022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 all subjects assessment average CD4 CD8 Cell Cell-mediated immunity clone coronavirus Coronaviruses Critical cytokine cytokine response Cytokines declined demonstrated dominant ELISPOT ELISpot assay envelope enzyme-linked immunosorbent epitope Epitopes Ex vivo female Frequency gamma interferon granzyme B greater GzmB highlight IFN-γ IL-2 immune immune modeling. Immunity immunodominant immunodominant epitope immunodominant epitopes induce Infection interferon interleukin interleukin 2 kill longitudinal male median membrane nucleocapsid observation other coronaviruses peaked persistence post-symptom onset potent predicted produced producing proliferative PSO reduced regions response robust SARS-CoV-1 SARS-CoV-2 seasonal coronavirus seasonal coronaviruses severe acute respiratory syndrome Coronavirus severe disease structural protein structural proteins subject survivor T cell T cell immunity T cell memory T cell response T cell responses T cells target cells Vaccine viral infections Year [DOI] 10.1128/jvi.00509-22 PMC 바로가기 [Article Type] Article
A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including OmicronOmicron을 포함한 모든 SARS-CoV-2 스파이크 변이체의 보존된 숨겨진 틈을 인식하는 나노바디 패널Article Published on 2022-07-062022-09-11 Journal: Communications Biology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] Accessibility Analysis antibodies antibody caused clone conserved Contact coronavirus COVID-19 detect diagnostics ELISA epitope Epitopes FIVE flow cytometric hidden Human Imbalance Infectious disease kinetic lateral flow Medicines Microscopy N-terminal domain nanobodies nanobody neutralize NTD omicron pandemic populations pseudotyped RBD Receptor-binding domain recognizing regions SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 spike proteins SARS-CoV-2 spike variant SARS-CoV-2 variant Spike proteins spike variants target the receptor-binding domain the spike protein Vaccines Viral viral infection VOCs western blotting [DOI] 10.1038/s42003-022-03630-3 PMC 바로가기 [Article Type] Article
Reverse genetics systems for SARS-CoV-2SARS-CoV-2에 대한 역유전학 시스템Review Published on 2022-07-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral drug BAC biosafety level Biosafety Level 2 BSL2 BSL3 caused characterization clone coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CPER feature functions genetics highlighting impair knowledge level 2 level 3 Limited mechanism mutant operation outbreak pandemic performed platform public health Research researcher researchers reverse genetics systems. SARS-CoV-2 severe acute respiratory syndrome Coronavirus TAR toolkit Viral particles viral pathogenesis Viral protein viral replication virus wild-type [DOI] 10.1002/jmv.27738 PMC 바로가기 [Article Type] Review
Increased memory B cell potency and breadth after a SARS-CoV-2 mRNA boostSARS-CoV-2 mRNA 부스트 후 증가된 기억 B 세포 효능 및 폭Article Published on 2022-07-012022-09-11 Journal: Nature [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] accompanied analysed antibody breadth Cell clone clones Cohort consequence conserved conserved regions convalescent individual disease domain dose effective Evolution expansion explain help increase in Increased individual Infection memory memory B memory B cell memory B cells Memory cells mRNA boost mRNA vaccine neutralized Neutralizing antibodies neutralizing antibody omicron Omicron variant potency PROTECT receiving Receptor-binding domain reduced Region respond SARS-CoV-2 second dose significantly increased the RBD these cell These cells These data Vaccine variant variant of SARS-CoV-2 variants [DOI] 10.1038/s41586-022-04778-y PMC 바로가기 [Article Type] Article
Analysis of mRNA vaccination-elicited RBD-specific memory B cells reveals strong but incomplete immune escape of the SARS-CoV-2 Omicron variantmRNA 백신 접종으로 유발된 RBD 특이적 기억 B 세포의 분석은 SARS-CoV-2 Omicron 변이체의 강력하지만 불완전한 면역 탈출을 보여줍니다Article Published on 2022-06-142022-09-11 Journal: Immunity [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] affinity analyzed antibodies B cells clone convalescent COVID-19 elicited expressed Germinal center high affinity IgG antibody Immune escape IMPROVE individuals Infection MBC MBCs memory B memory B cell memory B cells monoclonal mRNA mRNA vaccine mRNA vaccines neutralization neutralization potency Neutralizing omicron Omicron variant other variant other variants proportion provide RBD Recognition recognize reduction in reveal SARS-CoV-2 the SARS-CoV-2 vaccinated individuals vaccination variant variants VoC [DOI] 10.1016/j.immuni.2022.04.002 PMC 바로가기 [Article Type] Article
Human Superantibodies to 3CL pro Inhibit Replication of SARS-CoV-2 across Variants3CL pro에 대한 인간 초항체는 변이체 전반에 걸쳐 SARS-CoV-2의 복제를 억제합니다Article Published on 2022-06-132022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 변종, [키워드] 3CL pro 3CLpro Betacoronavirus betacoronaviruses binding blocked catalytic activity Cell cell-penetrating antibody clone conserved Contact E. coli effective enzyme Escherichia coli Host Human human cells human single-chain antibody variable fragments (HuscFvs) in vitro infect inhibited inhibitory investigated Major major protease (3CLpro) membrane omicron Omicron variants peptide phage predicted produced protease Replication residue residues Safe SARS-CoV-2 single-chain antibody superantibody. tested the SARS-CoV-2 variant virus replication was used were used wild type Wuhan [DOI] 10.3390/ijms23126587 PMC 바로가기 [Article Type] Article
Induction of high affinity monoclonal antibodies against SARS-CoV-2 variant infection using a DNA prime-protein boost strategyDNA 프라임-단백질 부스트 전략을 이용한 SARS-CoV-2 변이체 감염에 대한 고친화성 단일클론항체 유도Article Published on 2022-06-092022-09-11 Journal: Journal of Biomedical Science [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] Alpha alpha and delta variants angiotensin angiotensin-converting enzyme 2 animals Antibody titers approach approved best Beta Beta variant binding ability binding affinity boost broad spectrum clone coronavirus Coverage COVID-19 COVID-19 vaccination D614G Delta delta variant DNA ELISA Emergency use Endemic epitope epitope mapping Epitopes evaluated exhibit flu Gamma generate high affinity Human angiotensin-converting enzyme 2 include induction Infection Lambda monoclonal antibodies monoclonal antibody monoclonal antibody combination Multiple neutralization neutralization potency neutralize Neutralizing antibody titer neutralizing capacity neutralizing epitope neutralizing epitopes Outbreaks PROTECT RBD Receptor-binding domain regions SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant selected Spike protein the receptor-binding domain the spike protein therapeutic drug therapeutic effect therapeutic effects treat treated Treatment variant variants VoC VOCs were used Wuhan strain [DOI] 10.1186/s12929-022-00823-0 PMC 바로가기 [Article Type] Article
SARS-CoV-2 vaccination diversifies the CD4+ spike-reactive T cell repertoire in patients with prior SARS-CoV-2 infectionSARS-CoV-2 백신 접종은 이전 SARS-CoV-2 감염 환자에서 CD4+ 스파이크 반응성 T 세포 레퍼토리를 다양화합니다.Article Published on 2022-06-012022-09-12 Journal: EBioMedicine [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] activated analyzed B cell response CD4+ CD4+ T cell CD4+ T cells clone common cold coronavirus coronavirus Coronaviruses COVID-19 COVID-19 convalescent patient COVID-19 disease demonstrated elicit expansion foundation healthy donor identify immune response induce Johns Hopkins University Melinda Gate mRNA mRNA vaccination mRNA vaccine naïve natural infection NIH Patient patients Post-vaccine question reactive receptor recipients response SARS-CoV-2 SARS-CoV-2 antigens SARS-COV-2 infection SARS-CoV-2 vaccination Specific spike glycoprotein stimulated T cell T cell receptor the SARS-CoV-2 the vaccine vaccination Vaccine [DOI] 10.1016/j.ebiom.2022.104048 PMC 바로가기 [Article Type] Article
Highly multiplexed immune repertoire sequencing links multiple lymphocyte classes with severity of response to COVID-19Research article Published on 2022-05-142022-10-05 Journal: EClinicalMedicine [Category] 진단, 치료기술, [키워드] adaptive Adaptive immune response analyzed Antigen association Berlin cell counts cell types characterized clinical Clinical course Clinical management clone controls coronavirus coronavirus disease COVID-19 COVID-19 disease severity Critical cross-sectional decrease Disease progression disease severity distribution enrichment examined figure finding funding group healthy healthy control healthy subject heavy chain highest highlighting HLA immune Immune receptor Immune repertoires immune response Immunoglobulin IMPROVE increase in leukocyte lymphocyte measure Metadata Mild outbreak Peripheral blood Predictive Probability progression receptor SARS-CoV-2 scale sequence Sequencing severe COVID-19 severe COVID-19 disease severity subject subjects T cell T cell receptor T cells target the disease Therapies TRB TRD understanding variable with COVID-19 World Health Organization [DOI] 10.1016/j.eclinm.2022.101438 [Article Type] Research article